致土腥味链霉菌的拮抗菌筛选、鉴定及抑制作用研究

为筛选可抑制水产养殖中产土臭素（GSM）和2-甲基异莰醇（2-MIB）的链霉菌生长能力的益生菌，以嗜热一氧化碳链霉菌（Streptomyces thermocarboxydus）、蓝微褐链霉菌（S. cyaneofuscatus）和玫瑰黄链霉菌（S. roseoflavus）为指示菌株，进行抑菌活性筛选，并对具有抑菌活性菌株进行鉴定。本研究共筛选获得8株拮抗菌，结果表明其中2株为地衣芽孢杆菌属（Bacillus licheniformis），6株为枯草芽孢杆菌属（B. subtilis）。所筛菌株对供试链霉菌的生长均有抑制作用，其中2株可抑制嗜热一氧化碳链霉菌和玫瑰黄链霉菌的生长，另外6株对嗜热一氧化碳链霉的生长有抑制作用。筛选出的8株细菌可作为防控土腥味的益生菌，为生物控制和去除水产养殖中的土腥味提供了理论参考和实践依据。

Screening, identification and inhibition of antagonistic bacteria against off-flavor-producing Streptomyces

In order to screen probiotics that can inhibit the growth of Streptomycetes spp. that produce geosmin (GSM) and 2-methylisobcamol (2-MIB) in aquaculture, Streptomyces thermocarboxydus, S. cyaneofuscatus and S. roseoflavus were used as test strains to carry out inhibition experiments, and the screened strains were identified. The results showed that eight strains of probiotics had inhibitory effects on the growth of S. spp., 2 strains of them could inhibit the growth of S. thermocarboxydus and S. roseoflavus, meanwhile, other 6 strains affected the growth of S. thermocarboxydus. 2 strains belonged to Bacillus licheniformis and 6 strains belonged to B. subtilis.The 8 screened strains can serve as probiotics for preventing and controlling off-flavour, providing theoretical reference and practical basis for biological control and removal of off-flavour in aquaculture.