| 一种用高拷贝质粒载体制备BAC载体基本功能基因的新方法(英文) |
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| 作者单位： | 河北北方学院动物科技学院动物医学系 河北张家口075131(崔红玉，徐明举，薛永志)，河北省张家口市宣化区动物检疫站 河北张家口075100(王亚萍)，中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室 黑龙江哈尔滨150001(石星明，兰德松，王云峰，童光志) |
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| 摘 要： | Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAC.Method] With selecting a proper single restriction site,sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector.Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection.Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector,besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library.
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Research on the Construction of Bacterial Artificial Chromosome Vector DNA and the Potentials in Application |
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| Abstract: | Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAC.Method] With selecting a proper single restriction site,sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector.Result] The gene sequence of BAC vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection.Conclusion] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAC vector,besides that it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library. |
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| Keywords: | Bacterial artificial chromosome (BAC) High-copy plasmid BAC vector Single-copy plasmid |
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