重离子诱变鞘氨醇单胞菌选育毒死蜱高效降解菌株及应用

从青藏高原牦牛粪中分离出1株可降解毒死蜱的细菌,经形态和16SrDNA序列分析,鉴定其为鞘氨醇单胞菌属(Sphingomonas sp.)细菌。对其进行重离子诱变并筛选出菌株Sph-25-T1,该菌株对100mg.L-1毒死蜱的72h降解率达98.44%,比出发菌株提高27.37%。对Sph-25-T1进行传代培养,第5代时其对毒死蜱的降解率依然保持在90%以上。以喷洒毒死蜱1周后的不同土壤浸出液或蔬菜叶片做培养基时,Sph-25-T1对毒死蜱的降解率达到29.68%～78.40%。

Screening and Application of Strain of Sphingomonas sp. with High Degradation Rate of Chlorpyrifos by Heavy ion Beam Irradiation

A strain with the ability of chlorpyrifos degradation was isolated from the yak dung on Qinghai-Tibet Plateau,and identified as Sphingomonas sp.based on the phenotype and 16S rDNA sequence analysis.In present study,we used heavy-ion beam irradiation to mutagenize the strain and screened a mutant named Sph-25-T1.The experiment results showed that the strain Sph-25-T1 strongly degraded chlorpyrifos added in the medium,the degradation rate of 100 mg·L-1 chlorpyrifos added in medium was by 98.44% after 72h and 27.37% higher than that of the original strain.The strain Sph-25-T1 also degraded chlorpyrifos by 90% above after five generations cultivation.In the mediums of soil extract and vegetable leaves after spraying chlorpyrifos for one week,the strain Sph-25-T1 had the degradation rate of chlorpyrifos by 29.68%-78.40% after 84 h.