| 铁皮石斛杂交种组织培养技术试验 |
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| 引用本文： | 高燕,李泽生,耿秀英,李守岭,李桂琳,白燕冰. 铁皮石斛杂交种组织培养技术试验[J]. 云南热作科技, 2014, 0(2): 21-24 |
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| 作者姓名： | 高燕 李泽生 耿秀英 李守岭 李桂琳 白燕冰 |
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| 作者单位： | 云南省德宏热带农业科学研究所,云南瑞丽678600 |
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| 基金项目： | 云南省科技厅院所技术开发专项“石斛组培苗生产技术熟化与应用”（2012cf021） |
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| 摘 要： | 以铁皮石斛杂交种子为外植体的组织培养技术试验结果：铁皮石斛杂交种原球茎最佳诱导培养基为3/4Ma＋5%椰子汁；增殖分化培养基为3/4Ma＋6-BA 0.2 mg·L-1＋NAA 0.1 mg·L-1＋10%马铃薯泥＋2%香蕉泥；壮苗生根培养基为3/4Ma＋6-BA 0.2 mg·L-1＋NAA 1.0 mg·L-1＋8%马铃薯泥＋5%香蕉泥，生根率可达100%，移栽成活率在97%以上。
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| 关 键 词： | 铁皮石斛 种胚 组织培养 |
Experiment of Hybrid Dendrobii officmalis in Vitro |
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| GAO Yan,LI Ze-sheng,GENG Xiu-ying,LI Shou-ling,LI Gui-lin,BAI Yan-bing. Experiment of Hybrid Dendrobii officmalis in Vitro[J]. Journal of Yunnan Tropical Crops Science & Technology, 2014, 0(2): 21-24 |
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| Authors: | GAO Yan LI Ze-sheng GENG Xiu-ying LI Shou-ling LI Gui-lin BAI Yan-bing |
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| Affiliation: | (Dehong Institute of Tropical Agriculture, Ruili 678600, China) |
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| Abstract: | The results from experiment of hybrid Dendrobii officmalis seeds have shown that the optimum induced medi-um was 3/4 Ma plus 5% of coconut juice. 3/4Ma＋6-BA0.2 mg · L- 1＋NAA0.1 mg · L- 1＋10% of mashed potatoes＋2% of mashed bananas and 3/4Ma＋6-BA0.2 mg · L-1＋NAA1.0 mg · L-1＋8% for propagation culture and rooting respective-ly, with 100 percent of rooting inducing rate and up to 97%of survival rate after transplanting into field. |
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| Keywords: | Dendrobii officmalis embryo breeding tissue culture |
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