加味丹参饮抑制p38MAPK表达保护缺氧/复氧乳鼠心肌细胞损伤的实验研究

目的 观察加味丹参饮含药血清对缺氧/复氧（hypoxia/reoxygenation，H/R）乳鼠心肌细胞p38 MAPK蛋白表达的影响，以探讨其保护心肌的作用机制。方法 将20只SD乳鼠的心肌细胞进行原代培养，建立H/R模型并随机分为H/R组、SB203580（p38MAPK阻断剂组）、加味丹参饮含药血清组，正常心肌细胞组作为对照组。采用T淋巴细胞化学染色法鉴定心肌细胞，罗丹明B（Rhodamine B）染色法观察细胞形态，MTT比色法检测含药血清对乳鼠心肌细胞的毒性，Western-blot法检测MKK3（促分裂原活化蛋白激酶-激酶3）、MKK6（促分裂原活化蛋白激酶-激酶6）、p38MAPK、phospho-p38MAPK蛋白表达。结果 与正常血清对照组比较，H/R组MKK3、MKK6的蛋白表达增多，p38MAPK通路阻断剂SB203580和加味丹参饮均不能减少其表达；p38MAPK、phospho-p38MAPK在H/R时表达均增加（P<0.01），而SB203580和加味丹参饮含药血清均能减少其表达（P<0.01）。结论 加味丹参饮含药血清预处理可通过抑制缺氧，复氧心肌细胞p38MAPK信号通路发挥保护作用，抑制p38MAPK表达及其磷酸化，减轻心肌细胞损伤，起到保护心肌细胞的作用。

Experimental Study of Modified Jiawei Danshen Decoction on the Protection of Hypoxia/Reoxygenation Myocardial Cells Injury by Inhibition of p38MARK Expression in Neonatal Rats

Objective To observe the protective effect of modified Danshen Decoction containing serum on p38MARK expression in myocardial cells of hypoxia/reoxygenation (H/R) neonatal rats and ivvestigate its underlying molecular mechanisms. Methods Cardiomyocytes from 20 neonatal SD rats were primary cultured, and the built H/R models were randomly divided into H/R group, SB203580 group (p38MAPK inhibitor group), modified Danshen Decoction-containing serum group, and the normal cultured cardiomyocytes were as the control group. Cardiomyocytes were identified by T cell chemical staining method, and the morphologic changes in cardiomyocytes were observed by the Rhodamine B staining. Cytotoxicity of drug-contained sera was determined by the MTT method. Expression of MAP Kinase Kinase 3 (MKK3), MKK6, p38MAPK, and phospho-p38MAPK were measured by the Western blotting. Results Compared with the normal serum control group, the expressions of MKK3 and MKK6 were increased in H/R group, which was not altered by SB203580 and modified Danshen Decoction; The levels of p38MAPK and phospho-p38MAPK at H/R were increased, while the p38MAPK and phosphop-38MAPK expression in SB203580 and modified Danshen Decoction groups were decreased. Conclusion The modified Danshen Decoction could protect cardiomyocytes and decrease H/R-induced myocardial cells injury by inhibiting the expression and phosphorylation of p38MAPK through the inhibition of its p38MAPK signaling pathway.