愈痫灵方及其加味方对戊四氮致痫鼠海马组织中TLR4、MyD88表达的影响

Effects of Yuxianling and its Modified Decoction on the Expressions of TLR4 and MyD88 in the PTZ-induced Epileptic Rats

Objective To explore the influence of Yuxianling and its modified decoction on the expressions of Toll-like receptor 4 (TLR-4) and the myeloid differentiation factor 88 (MyD88) in hippocampus of epileptic rats. Methods The 130 male healthy Sprague-Dawley rats were randomly divided into control group(n=10), sham-operation group (n=10), pentylenetetrazole (PTZ) group (n=110). The sham operation group were injected with equivalent saline, and the rats of PTZ group were intraperitoneal injected with pentylenetetrazole. The standard epileptic rats were randomly divided into model group, valproate group, Yuxianling decoction (YXL) group, modified Yuxianling decoction (MYXL) group, 10 rats in each group. The expression of TLR4 mRNA protein in rat hippocampus were detected by RT-PCR, and the expression of MyD88 protein was tested by immunohistochemical method.Results The expression of TLR4 mRNA and MyD88 protein in model group was significantly higher than control group and sham-operation group (P<0.01), the expressions in YXL, MYXL and valproate groups comparing with the model group were statistically significant (P<0.01), the expressions in YXL and MYXL groups comparing with the valproate group were statistically significant There was no significant differences between control group and sham-operation group(P>0.05); the expression of TLR4 mRNA and MyD88 protein in YXL and YXLH was much weaker than that in valproate group (P<0.05). Conclusion The increased expression of TLR4 mRNA and MyD88 protein in epileptic rats hippocampus may play an promotion role on the development of the hippocampus injury. Yuxianling decoction can down regulate the expression of TLR4 mRNA and MyD88 protein and lessen the pathologic changes of hippocampus.