| 黄鳝藤黄微球菌的分离鉴定及药敏试验 |
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| 引用本文： | 彭彬,杨光友,陈晓利,占爱思,何明莉.黄鳝藤黄微球菌的分离鉴定及药敏试验[J].上海水产大学学报,2011(3). |
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| 作者姓名： | 彭彬 杨光友 陈晓利 占爱思 何明莉 |
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| 作者单位： | 四川农业大学动物科技学院水产养殖学系;四川农业大学动物医学院预防兽医学系; |
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| 基金项目： | 教育部《长江学者和创新团队发展计划》创新团队项目(IRTO848) |
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| 摘 要： | 为探讨黄鳝(Monopterus albus)出血病病原菌的种类及耐药情况,采用动物回归和细菌常规分类鉴定方法及16S rDNA基因系统发育学分析等手段,从四川名山地区黄鳝出血病病料中分离到具有致病性的菌株(HM1),经人工感染实验证实该菌为黄鳝出血病的病原菌。动物致病性试验显示,该分离株对泥鳅和小白鼠不致病,对云斑有较弱的致病力。对该分离株的形态和生理生化指标进行分析,试验结果与报道的藤黄微球菌一致,为革兰氏阳性、球菌,无运动力,多成双、四联或簇状排列,不形成链状排列,不形成芽孢;接触酶和明胶液化阳性;乳糖、甘露醇、葡萄糖、七叶苷、硝酸盐还原、精氨酸双水解阴性等特征。采用细菌16S rDNA基因通用引物对该菌的16S rDNA基因进行PCR扩增、克隆测序,得到1条长度为1 382 bp的核苷酸序列(GenBank登录号为:HM044913)。该序列经RDP(Ribosomal Database Project)数据库在线Classifer分析,结果表明该菌株属于微球菌属细菌。以该菌16SrDNA序列和GenBank数据库内同源性较高的细菌16S rDNA序列进行同源性比对分析和构建系统发育树,结果显示分离...
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| 关 键 词： | 黄鳝 出血病 藤黄微球菌 |
Isolation and identification of Micrococcus luteus in rice field eel(Monopterus albus) in Sichuan Province |
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| PENG Bin,YANG Guang-you,CHEN Xiao-li,ZHAN Ai-si,HE Ming-li.Isolation and identification of Micrococcus luteus in rice field eel(Monopterus albus) in Sichuan Province[J].Journal of Shanghai Fisheries University,2011(3). |
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| Authors: | PENG Bin YANG Guang-you CHEN Xiao-li ZHAN Ai-si HE Ming-li |
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| Institution: | PENG Bin1,2,YANG Guang-you2,CHEN Xiao-li1,ZHAN Ai-si2,HE Ming-li1(1.College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,Sichuan,China,2.College of Veterinary Medicine,China) |
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| Abstract: | This experiment was conducted to analyse the species and drug resistance of hemorrhagic pathogens from Monopterus albus.One hemorrhagic disease pathogens(HM1) of M.albus from Mingshan in Sichuan was isolated using bacteria classification and identification technique,and was identified according to the morphological,physiological and biological characteristics and the phylogenetic analysis.On pathogenicity test of animals,the experimental Ictalurus nebulosus demonstrated weak pathogenic symptom,but not any p... |
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| Keywords: | Monopterus albus hemorrhagic disease Micrococcus luteus |
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