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铜藻粗多糖酶法提取工艺的优化及其抗氧化活性研究
引用本文:刘丽佳,何淑婷,姜露,薛露,杨玲,曲有乐.铜藻粗多糖酶法提取工艺的优化及其抗氧化活性研究[J].浙江水产学院学报,2013(6):503-507.
作者姓名:刘丽佳  何淑婷  姜露  薛露  杨玲  曲有乐
作者单位:浙江海洋学院食品与医药学院,浙江舟山316022
基金项目:舟山市科技计划项目(2012C33004);浙江省自然科学基金(LQl3c200002);2012年浙江海洋学院大学生科技创新项目
摘    要:通过正交试验法对铜藻中粗多糖的酶法提取工艺进行优选,并测定其1,1-二苯基苦基苯肼(DPPH)自由基的清除能力、超氧阴离子自由基(O2-)及抗脂质过氧化能力;优化后铜藻粗多糖提取率为5.61%,具有一定的DPPH自由基清除能力、抗脂质过氧化能力、O2-清除能力。

关 键 词:铜藻  多糖  正交实验  抗氧化活性

Study on Polysaccharide Extraction and its Antioxidative Activity from Sargassum herneri
Institution:LIU Li-jia, HE Shu-ting, JIANG Lu, et al (Food and Pharmacy School of Zhejiang Ocean University, Zhoushan 316022, China)
Abstract:Polysaccharide was extracted by enzymes from brown algae(Sargassum herneri) using orthogonal experiment in this study, and antioxidative activity of extracted polysaccharide was investigated in vitro. An-tioxidative activity extracted polysaccharide was evaluated with the scavenging capacity of DPPH, free radical (O2-) and lipid peroxidation in vitro. After optimized the copper extraction rate of algal polysaccharides was 5.61%and extrated polysaccharide possess the capacities of scavenging DPPH and free radical(O 2-) and protect lipid from peroxidation.
Keywords:Sargassum herneri  polysaccharide  orthogonal experiment  antioxidative activity
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