Characterization of the systemic disease and ocular signs induced by experimental infection with Chlamydia psittaci in cats |
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| Institution: | 1. Université de Tunis El Manar, Ecole Nationale d''Ingénieurs de Tunis, Unité de recherche énergétique et environnement, 1002, Tunis, Tunisia;2. Esprit School of Engineering, Tunis, Tunisia;3. Université de Tunis El Manar, Institut Préparatoire aux Etudes d''Ingénieurs d''EL Manar, 2092, Tunis, Tunisia;1. Faculty of Electrical Engineering, Mechanical Engineering and Naval Architecture, University of Split, Split, Croatia;2. Institute of Information Technology, Viet Nam Academy of Sciences and Technologies, Hanoi, Viet Nam;3. Research and Development Center, Duy Tan University, Da Nang, Viet Nam;4. Department of Mathematic and Informatics, Thang Long University, Hanoi, Viet Nam;5. Faculty of Electronics Engineering, Thai Nguyen University of Technology, Thai Nguyen, Viet Nam;1. School of Medical and Applied Sciences, Central Queensland University, Rockhampton, Queensland 4702, Australia;2. School of Science and Health, Western Sydney University, Hawkesbury, Penrith, NSW 2751, Australia;1. Central Queensland University, School of Medical and Applied Sciences, Rockhampton, Queensland 4702, Australia;2. University of Western Sydney, School of Science and Health, Penrith NSW 2751, Australia;1. Texas Children''s Hospital, Baylor College of Medicine, Houston, Texas;2. Children''s Nutrition Research Center, Baylor College of Medicine, Houston, Texas;1. School of Clinical Sciences, University of Bristol, Bristol, BS8 1TD, UK;2. Section of Immunity, Infection and Inflammation (Ocular Immunology), Division of Applied Medicine, School of Medicine and Dentistry, Institute of Medical Sciences, Foresterhill, University of Aberdeen, AB25 2ZD, Scotland, UK;3. Ocular Immunology Program, Centre for Ophthalmology and Visual Science, The University of Western Australia, Western Australia, 6009, Australia;4. Centre for Experimental Immunology, Lions Eye Institute, Nedlands, Western Australia, 6009, Australia;5. School of Cellular and Molecular Medicine, University of Bristol, Bristol, BS8 1TD, UK;6. National Institute for Health Research Biomedical Research Centre at Moorfields Eye Hospital NHS Foundation Trust and UCL Institute of Ophthalmology, London, EC1V 2PD, UK |
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| Abstract: | In addition to the commonly reported ocular signs, Chlamydia psittaci infection of kittens resulted in fever, lethargy, lameness and reduction in weight gain following ocular instillation of virulent organisms. The appearance of these systemic signs was late with respect to the appearance of ocular symptoms and occurred simultaneously with increasing levels of chlamydia-specific IgG. Measurement of acute phase reactants and IL-6 in plasma indicated that both became elevated concurrent with or slightly after the appearance of fever and remained elevated after the fever began to resolve. Preliminary data also indicated that infectious C. psittaci was present in the blood stream during this time period. The results of ocular instillation of three different levels of C. psittaci (103.8, 102.8 and 101.5 TCID50) indicated that the frequency of infection and the severity of ocular signs were diminished in the group receiving the lowest dose. However, the magnitude of systemic disease was similar in all animals which exhibited clinical signs, irrespective of the dose administered. The immune response to infection included elementary body (EB)-specific lymphocyte proliferation as well as the development of EB-specific IgG and IgM antibodies. The predominant antibody response was to a 45 kDa protein, the major outer membrane protein (MOMP), lipopolysaccharide (LPS), a 58 kDa doublet and 32 and 16–19 kDa proteins. |
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