| 白花泡桐茎和叶差异表达的cDNA-SRAP分析 |
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| 引用本文： | 茹广欣,周霜晴,朱秀红,刘小囡,王鋆瑞.白花泡桐茎和叶差异表达的cDNA-SRAP分析[J].河南农业大学学报,2017,51(4). |
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| 作者姓名： | 茹广欣 周霜晴 朱秀红 刘小囡 王鋆瑞 |
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| 作者单位： | 河南农业大学林学院,河南郑州,450002 |
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| 基金项目： | 国家科技支撑计划专题，大学生创新创业训练计划 |
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| 摘 要： | 以白花泡桐茎和叶为材料,利用cDNA-SRAP分子标记技术,对其进行基因表达的差异分析。从64对引物中筛选出25对扩增效果较好的引物对进行扩增,检测得到36个差异片段,2次PCR扩增重复率为66.8%。经挑选回收、克隆和测序,最终测得23条差异片段序列,长度主要在400~1 700bp之间。经BLASTX进行比对和功能分析,16条与已知功能基因有较高同源性,包含能量与代谢(34.78%)、蛋白质合成(8.70%)、细胞壁相关(4.35%)、信号传导与胁迫响应(8.70%)及复合影响(13.04%)等多个方面;7条与未知功能基因序列有较高同源性。
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| 关 键 词： | cDNA-SRAP 白花泡桐 差异片段 功能分析 |
Differential expression analysis of stem and leaf in Paulownia fortunei by cDNA-SRAP |
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| RU Guangxin,ZHOU Shuangqing,ZHU Xiuhong,LIU Xiaonan,WANG Yunrui.Differential expression analysis of stem and leaf in Paulownia fortunei by cDNA-SRAP[J].Journal of Henan Agricultural University,2017,51(4). |
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| Authors: | RU Guangxin ZHOU Shuangqing ZHU Xiuhong LIU Xiaonan WANG Yunrui |
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| Abstract: | The stem and leaf of Paulownia fortunei to analyze the differential gene expression with the method of cDNA-SRAP.Among the 64 pairs of primers,25 pairs of primers were screened with good amplification effect,and 36 differential fragments were detected.The repetition rate of the two times PCR amplification was about 66.8％.After being selected,cloned,and sequenced,23 fragments of the differential sequence were measured,and the length was between 400 and 1 700bp.According to the genomic database,sequence analysis showed that 16 fragments have significant homologous nucleotide sequence and 7 fragments with unknown function gene sequence has high homology. |
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| Keywords: | cDNA-SRAP Paulownia fortunei differential fragments functional analysis |
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