VEGF在绵羊生殖管道中的表达规律

以不同发情周期雌性绵羊子宫、输卵管为研究对象,采用免疫组织化学技术,针对血管内皮生长因子（VEGF）在绵羊子宫、输卵管的表达、定位和变化规律进行了检测,同时应用相关图像分析软件对抗原染色强度进行了定量分析。结果表明：输卵管在发情0～15d,VEGF表达量在第9天达到峰值后经历波动逐渐下降过程,输卵管内膜上皮细胞是VEGF抗原的主要靶细胞;而子宫角在发情0～15d,VEGF表达量在第5天达到峰值后经历波动逐渐下降过程,子宫内膜固有层及腺体周围细胞为VEGF抗原的主要靶细胞。该研究结果为绵羊生产中进一步提高受胎率和妊娠率及频密产羔等技术的应用提供了科学依据。     

雌性牦牛主要生殖器官中DBI的表达与定位分析

为加深对DBI基因功能的探究,揭示其在牦牛生殖生理过程中的作用。本试验采集健康母牦牛（3～6岁）繁殖周期不同阶段（卵泡期、黄体期和妊娠期）的卵巢、输卵管和子宫组织,共分为9组,每组设置3个生物学重复。采用实时荧光定量PCR （qRT-PCR）和蛋白免疫印迹技术（Western blotting,WB）检测牦牛DBI在繁殖周期不同阶段雌性牦牛生殖器官中的相对表达量;通过免疫组织化学方法（immunohistochemistry,IHC）定位DBI蛋白在雌性牦牛卵巢、输卵管和子宫中的分布情况。结果表明：1） qRT-PCR结果显示,在卵巢中,DBI在黄体期和妊娠期的表达量显著高于卵泡期（P<0.05）;在输卵管中,DBI在黄体期表达量显著高于卵泡期和妊娠期（P<0.05）;在子宫中,DBI在妊娠期表达量最高,黄体期次之,卵泡期最低。2） Western blotting结果表明,在卵巢中,DBI在妊娠期的水平显著高于卵泡期和黄体期（P<0.05）;在输卵管中,DBI在黄体期水平显著高于卵泡期和妊娠期（P<0.05）;在子宫中,DBI在妊娠期水平明显高于黄体期和卵泡期（P<0.05）。3） IHC结果显示,DBI主要位于生殖上皮细胞、颗粒细胞、卵泡膜细胞、黄体细胞、输卵管上皮细胞、子宫基质细胞和子宫腺中。DBI在牦牛繁殖周期不同阶段的卵巢、输卵管和子宫组织中的表达量存在显著差异,说明其参与牦牛生殖生理调控,为进一步挖掘DBI基因在高原哺乳动物生殖方面的研究奠定相应的理论基础。     

Oocyte Transfer as an Analytical Tool:Vintage Experiments in Domestic Farm Animals

Four transplant studies are described that focus on fertilisation and early development or the progression of unfertilised oocytes (eggs) in the oviduct.(1) Pig eggs transplanted from ovulations induce...     

神经元特异性烯醇化酶在大鼠动情周期子宫中的分布

选择健康、未产、体重220-250g成年雌性SD大鼠，采用阴道涂片方法鉴定其动情周期，并采用免疫组织化学SP法研究了神经元特异性烯醇化酶（NSE）在大鼠动情周期子宫内分布的变化规律。结果显示，子宫各层均有不同程度NSE免疫阳性产物分布，并随动情周期的变化表现出一定的规律：各期子宫内膜黏膜上皮细胞、腺上皮细胞、基质细胞、血管内皮细胞及肥大细胞等NSE免疫阳性细胞数量变化不大，但着色深浅有一定差别，动情期着色最深，动情后期、动情间期、动情前期着色依次减弱；肌层和子宫外膜中，动情期着色最深，动情后期着色最浅，动情间期表达量明显回升，动情前期比动情期着色稍浅。表明，NSE在子宫中的分布可能受性类固醇激素的调节。     

Ova transport and fertility after resection of the oviductal isthmus in pigs

The effect upon the reproduction of total resection of the oviductal isthmus, including the uterotubal and ampullary-isthmus junctions followed by end-to-end ampullo-cornual anastomosis was studied in pigs. Normal cycling gifts of proved fertility were submitted to bilateral isthmus deletion (Group I). Other gilts were submitted only to unilateral isthmic resection, while their contralateral medial isthmus was transversally cut and reanastomosed (Group II). A significantly lower nidation index was obtained after bilateral isthmic resection. The gilts in Group I which did not become pregnant and those in Group II were successfully mated during forthcoming standing oestruses, and were in association to that slaughtered on days 3, 4, or 5 of the cycle, and the characteristics and location of ova were determined. Spermatozoa fertilized the ova, and those cleaved normally in the isthmic-resected tube as well as in the sham-operated oviducts. The isthmic-resected oviduct, however, did not transport cleaved ova into the uterus at the time expected as normal. The findings suggest that the fertilized pig ova cannot reach the uterus at the normal expected time in case of total absence of the isthmic part of the oviduct, resulting in impaired fertility.     

荆豆凝集素-1受体在犬子宫内的分布与激素调节

采用组织化学方法对荆豆凝集素-1(ulex europaeus agglutinin-1,UEA-1)受体在发情周期和早期妊娠犬子宫内的分布以及激素调节进行了研究。结果显示,UEA-1受体主要存在于犬子宫内膜腔上皮和腺上皮,其表达量随妊娠阶段的不同而发生动态性变化。UEA-1受体在休情期犬子宫内未见表达,而在发情期犬子宫内膜腔上皮和腺上皮内的表达量很高。在早期妊娠犬子宫内,在妊娠第6天和12天犬子宫内的表达量较低,此后其表达量逐渐增加,在妊娠第17天时,UEA-1受体在子宫内膜腔上皮和腺上皮内的表达量达到峰值,此后其表达量又逐渐下降,在妊娠第23天时未见表达。注射雌激素可明显促进卵巢切除后犬子宫内膜UEA-1受体的表达。结果表明,犬子宫内UEA-1受体的分布与胚胎着床前胚胎与子宫内膜之间的黏附和植入有关,UEA-1受体在犬子宫内的表达受母体分泌的激素所调控。     

Uterine Expression of WNT7A and β-catenin After Induction of Oestrus in Sheep

WNT7A and β-catenin localisations and roles in regulating periimplantation ovine conceptus development under natural estrous conditions have been elaborated.However,their locations and expression patterns have not been reported under induction of oestrus.The localisation,expression and function of WNT7A and β-catenin in the uterine tissues of the early pregnant and non-pregnant sheep on days 10,12,14,16 and 18 following artificial induction of oestrus were investigated by means of in situ hybridisation,real-time RT-PCR,immuno-histochemistry and western blotting methods.WNT7A and β-catenin mRNA and protein were both restricted to the apical surfaces of the uterine luminal epithelium (LE) and glandular epithelium (GE).In pregnant sheep,protein localisation of WNT7A and β-catenin was observed both in the endometrial LE and GE.Their staining presented on day 10,increased between day 12 and day 16,and decreased on day 18.WNT7A and β-catenin mRNA and protein expression increased initially and then decreased from day 10 to day 18,peaking on day 16,and β-catenin reaching a peak on day 18 in the uterine tissues of pregnant sheep (p0.05).By contrast,no significant changes in WNT7A and β-catenin mRNA and protein expression levels were observed from day 10 to day 18 of the oestrus cycle in the uterine tissues of non-pregnant sheep (p0.05).Additionally,WNT7A and β-catenin mRNA and protein expression levels in the uterine tissues of the early pregnant sheep were significantly higher than those of non-pregnant sheep (p0.05).Treatment of endometrial epithelial cells with WNT7A increased the mRNA expressions of β-catenin,c-myc and Cyclin D1.These results provided an underlying mechanism of periimplantation ovine conceptus development under induction of oestrus.     

子宫蓄脓犬的子宫和卵巢组织的显微及超微结构观察

应用超薄切片、光学及电子显微镜检查等技术，对临床上96例犬子宫蓄脓病例卵巢和子宫作显微、超微结构的观察，探讨其发病机制。结果表明，大部分病犬的子宫内膜腺体出现不同程度的囊性增生（CEH）和炎症变化；卵巢有持久黄体或囊性黄体。开放型病犬多表现子宫壁增厚；闭锁型多表现子宫内膜萎缩，子宫壁极薄，子宫内膜腺体发生CEH时，其上皮表现鳞状化生和腺瘤样病变。此外，闭锁型病例其子宫与卵巢肉眼可见的、显微及超微组织病理学变化均较开放型严重。