新疆驴冷冻精液的研究

试验旨在探究新疆驴冷冻精液的最佳稀释保护液与处理方法。以假阴道采集新疆驴精液,并用5种不同稀释保护液稀释冷冻后,选取其中较为理想的稀释液作为对照组,再通过分别添加4%、5%、6%、8%甘油处理试验,最后再选用添加甘油后冷冻效果最优的稀释液为对照来进行离心浓缩试验。结果表明：①5号稀释液冷冻—解冻精液后,其活力、顶体完整率要显著高于其他4种稀释液（P<0.05）;②以5号稀释液为对照组,添加6%甘油(7号稀释液)冷冻后,精子的活力、顶体完整率要显著高于添加4%、5%、8%甘油组（P<0.05）;③以7号稀释液为对照组,通过离心浓缩,精液在冷冻后其活力和精子顶体完整率都显著提高（P<0.05）。结果提示,将驴精液用6%葡萄糖、2%乳糖、6%甘油为主的稀释液稀释,并经500 r/min离心浓缩10 min处理后,其冷冻效果最好。     

邻苯二甲酰亚胺乙氧基环甘油硫代磷脂缀合物的合成

以碘活化的六乙基亚磷酰三胺为磷酰化及环化试剂 ,经“一锅法”合成了邻苯二甲酰亚胺乙氧基环甘油硫代磷脂缀合物 ,收率达 90 %左右     

Incorporation of 14C into methionine as an intermediate for phytosiderophore biosynthesis in barley

In order to verify the precursory role of methionine (Met) in the biosynthesis of the mugineic acid family of phytosiderophores (MAs), feeding experiments of ¹⁴C-Iabeled compounds to barley (Hordeum vulgare L. cv. Minorimugi) roots grown hydroponically were conducted. When both ^l4C-Glucose (Glc) and unlabeled Met were fed to segmented roots, ¹⁴C was incorporated into Met and MAs in the roots. Molar-radioactivity of Met was higher than that of the amino-butanoic-acid unit in MAs in the roots. When ^l4C-Glc and unlabeled homoserine (Hse) were fed to decapitated roots, l4C was incorporated into Met but not into Hse. Therefore, it was considered that Hse might not be a major precursor of MAs. In addition, ¹⁴C was incorporated into Met and MAs in the roots when both ¹⁴C-glycerol (Gol) and unlabeled Met were fed to segmented roots. It is suggested that MAs may be synthesized from Glc via Met, bypassing Hse, and that the MAs biosynthesis may involve an unknown pathway associated with Gol and leading to Met.     

小尾寒羊冷冻精液制作保存技术

在制作小尾寒羊冻精颗粒时，对其冷冻稀释液和甘油浓度进行了研究。结果表明，2＃稀释液效果最好（P＜0．05），甘油浓度为6％时的冷冻效果最佳（P＜0．01）；在40－50℃解冻后，其活力可达0．5以上。     

几种冷冻保存伊氏锥虫方法的比较试验研究

本文用液氮冷冻法比较了不同保护剂和不同冻存途径对伊氏锥虫云南株进行保存后，虫株在存活率、活力、感染力、致病力和药敏性方面的变化。我们发现在以二甲基亚砜为主要保存剂时，二甲基亚砜浓度低对保存效果不利，而浓度为５％时，保存７１２天后，虫体成活率仍达９６％；当以甘油为保存剂时，若其浓度低虫体成活率低，当浓度为２０％时，保存３６５天后虫体存活率有９１．４％。在不同冻存途径试验中发现，先在液氮气相中距液氮面４ｃｍ以上处放置１ｈ，再降到距液氮面４ｃｍ处放置１ｈ，然后进入液氮效果最好。用此种保存方法和冻存途径后，虫体活力强，其感染力、致病性和药敏性都没有发生明显变化。     

丝素-海藻酸钠-甘油共混膜的制备及性能研究

以丝素蛋白为原料,海藻酸钠和甘油作为添加剂,考察丝素蛋白溶液、海藻酸钠溶液的浓度和甘油的加入量对膜的厚度、断裂伸长率以及水蒸气迁移速率的影响,并通过正交试验来确定丝素-海藻酸钠-甘油共混膜的最佳配比.试验结果表明,最佳配方为丝素溶液浓度3%,海藻酸钠溶液浓度12.5 mg·mL-1,甘油加入量0.5 mL.在此条件下,膜的厚度、断裂伸长率及水蒸气迁移速率分别为0.075 mm、45.53%和13.76 g·m-2·h-1.     

Effects of oral isosorbide and glycerol on intraocular pressure,serum osmolality,and blood glucose in normal dogs

Objective To evaluate and compare the effects of oral isosorbide and glycerol on intraocular pressure (IOP), serum osmolality (SOSM), and blood glucose (BG) in normal dogs. Methods Ten normal dogs were administered an oral dose of either isosorbide (1.5 g/kg), glycerol (1.5 g/kg) or control (water, 2 mL/kg) in a double blind protocol. Prior to dosing, baseline IOP, SOSM, and BG were measured in all dogs. IOP was subsequently evaluated every 30 min for 6 h post‐dosing. BG and SOSM were reassessed at times 1, 2, 4, and 6 h post‐dosing. After 1‐week washout periods, every dog was subject to each of the three treatments. The dogs were held NPO for 4 h after dosing. Results The maximal decrease in IOP was 17% by 1 h and 13.5% by 30 min after glycerol and isosorbide administration, respectively. However, the overall changes in IOP were not significant when compared to the controls. SOSM increased above baseline after dosing with glycerol but decreased after isosorbide, which difference was significant at 1, 2, and 4 h post‐administration. BG significantly increased after administration of glycerol relative to the control but was not significantly affected by isosorbide. Conclusions Neither glycerol nor isosorbide significantly affected IOP when compared to the control. However, glycerol induced significant elevations in both BG relative to the control and SOSM relative to isosorbide.     

Genetic variation of in vivo muscle glycerol,glycogen, and pigment in Danish purebred pigs

Heritability values of glycerol, glycogen and pigment concentrations measured on muscle biopsy samples from longissimus dorsi obtained from 85?kg boars of Danish purebred pigs (Landrace, Yorkshire, and Duroc) and their genetic correlations to performance and meat quality traits were calculated. The heritability values of glycogen, pigment, and glycerol were 0.38±0.02, 0.17±0.02, and 0.065±0.016, respectively. Glycogen was negatively related to the feed conversion ratio (FCR) (r _g =?0.25±0.06) and ultimate meat pH (r _g =?0.41±0.04), and positively to the carcass meat percentage (r _g =0.35±0.04), L* (lightness of meat, r _g =0.32±0.04) and a* (redness of meat, r _g =0.12±0.03). Pigment was positively related to the FCR (r _g =0.12±0.05), the meat percentage (r _g =0.28±0.05), and a* (r _g =0.59±0.04), and negatively to the L* (r _g =?0.46±0.06). The concentrations of pigment and glycogen (r _g =0.27±0.05) were interrelated. Based on the heritability values and the signs of the genetic correlations the present data suggest that it is possible to select for higher ultimate pH and improved colour by selection for either lower muscle glycogen or higher pigment concentration, respectively. However, the positive genetic correlation between these two traits may restrict the efficiency of simultaneous selection for lower glycogen and higher pigment. All genetic parameter estimates presented are calculated across three purebreds. Values for each breed are warranted for use in pig production. However, this requires more animals per breed.