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1.
ABSTRACT: Chub mackerel (34–35 cm, approximately 500 g), which were caught by fishing with a rod and line at the Bungo Channel, Oita prefecture, were rested overnight in a fish preserve and either killed by decapitation (control group) or allowed to struggle in air for 30 min (struggled group). Muscle samples were excised every 4 h, and measurements on breaking strength and histological observations were done for both groups. The breaking strength of muscle in the control group was significantly higher than that in the struggled group, whereby a decrease in breaking strength was delayed for 12 h compared to the struggled group. Light microscopy showed space extension among muscle cells in association with a decrease in breaking strength. Especially in the struggled group, the extended area was larger and the difference in area was significant at the time when breaking strength showed a significant difference. Using electron microscopy, the extended area showed cut and/or disappeared collagen fibrils. From these results, it was demonstrated that struggling to death promoted the degradation of collagen fibrils and the weakening of connective tissue and, resultantly, led to the faster softening of muscle of chub mackerel.  相似文献   
2.
研究了陆地棉主要性状对混选—混交育种体系的响应。两轮群体提高了产量、早熟性和降低了麦克隆值的平均数;扩大了纤维品质的变幅;增加了高产株率,但对改进抗枯黄萎病性不明显。一轮群体的效果低于二轮。两次混交扩大了产量和纤维品质的变幅,增加了正末端的个体频率。一次混交的作用不及两次。早代混选相对缩小了群体变幅。一次混交后再经两代开放授粉和对一轮群体再混选,均表现出有益的反响。  相似文献   
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4.
Multiplex PCR assay for the detection of pathogenic Aeromonas hydrophila   总被引:1,自引:0,他引:1  
  相似文献   
5.
A nested polymerase chain reaction (PCR) system was developed for the detection of Flexibacter maritimus from fish tissue. The total procedure for the diagnosis of marine flexibacteriosis, from the point of DNA extraction to the electrophoretic analysis, can be performed in < 4 h. This was achieved by the combination of a short thermal cycling programme with a rapid DNA extraction procedure. The assay was extremely sensitive, capable of detecting as few as 75 cfu mg(-1) fish tissue. The accuracy of the nested PCR was confirmed under field conditions using tissue samples recovered during 1993-2002 from fish suffering marine flexibacteriosis. The nested PCR method proved to be efficient for the rapid and sensitive detection of F. maritimus from fish tissues and can be used for routine diagnosis of the disease caused by this pathogen.  相似文献   
6.
Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL−1 of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g−1 tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.  相似文献   
7.
[目的]考察浸矿剂硫酸铵(NH4)2SO4胁迫下稀土离子钇的毒性研究。[方法]选择蚯蚓作为土壤生态环境污染指示生物,采用滤纸接触法研究硫酸铵胁迫下稀土钇( Y)对蚯蚓的急性毒性效应。[结果]①稀土钇单一染毒,蚯蚓的48 h 半致死率浓度为 LC50=213.41 mg/L、24 h半致死率浓度为 LC50=322.63 mg/L。②硫酸铵单一染毒下,蚯蚓的48 h半致死率浓度为 LC50=13.89 g/L、24 h半致死率浓度为LC50=15.05 g/L。③低浓度10 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度LC50=198.65 g/L、24 h 半致死率浓度 LC50=399.85 g/L;中浓度14 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度 LC50=167.3 mg/L、24 h半致死率浓度 LC50=256.73 mg/L;高浓度20 g/L的硫酸铵与稀土钇复合染毒,蚯蚓的48 h半致死率浓度 LC50=31.03 mg/L、24 h的LC50=127.65 mg/L。[结论]低浓度的硫酸铵降低稀土钇对蚯蚓的毒性,产生一定的拮抗作用。中浓度的硫酸铵增加稀土钇对蚯蚓的毒性,产生较明显的协同作用。高浓度硫酸铵显著增加了稀土钇对蚯蚓的毒性。稀土钇染毒下蚯蚓死体更易断裂,而活体对针刺反应相对不灵敏。  相似文献   
8.
The decomposition of oak leaf litter was studied by means of a litterbag experiment in an oak forest in the Netherlands. The contribution of microbial activity and leaching to weight loss and element dynamics during the first 6 weeks of decomposition was investigated by means of frequent respiration measurements and extractions of the litter and by a qualitative comparison of throughfall and litter percolation water chemistry. The oak-leaf litter lost 9.3% of its initial dry weight during the first 6 weeks. In total, 90% of this observed weight loss was explained by the processes studied. About 5.9% (64% of the total) of this weight loss was attributed to microbial tespiration and 0.5% (5%) to the loss of inorganic solutes. Leaching of dissolved organic compounds was estimated to account for 2.0% (21%). The results indicated a fast leaching of K and Cl out of the fresh litter during the first 2 weeks, while Mg, Fe, Mn, Si, ortho P, and dissolved organic N were released at a much lower rate. At the same time, small amounts of H+, NH inf4 sup+ and NO inf3 sup- were retained in the litter.  相似文献   
9.
Dynamic Monitoring of the Mud   总被引:2,自引:0,他引:2  
[Objective] The research aimed to provide basic files and theoretical guidance for constructing sluicing-siltation dam using soil with high clay content soil.[Method] The soils of Dagou basin near Xiwu Village of Baishui County,Shaanxi Province were taken as experimental materials.pvc pipes with same height and diameter were used to construct testing model for dynamically determining settlement,shear strength,wet density of grouting bulk under 2 different grouting speeds(15 cm/d and 25 cm/d).[Result] Under different grouting speeds,general change trend was similar during grouting course.The subsidence,deformation,shear strength and wet density increased with the increase of grouting speed.Five or six days after grouting,daily displacement under 25 cm/d grouting speed was fewer than that under 15 cm/d grouting speed.[Conclusion] The increase of grouting speed could shorten the time for reaching the same subsidence,deformation,shear strength and wet density and increased displacement at the initial stage of grouting,however,with the increase of grouting time,lower grouting bulk was bad for displacement at later grouting period because it was near impermeable layer.  相似文献   
10.
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