Wound healing potential of Tragia involucrata extract in rats

The methanol extract of the roots of Tragia involucrata topically tested at doses of 100 and 200 mg/kg exerted significant wound healing effect in Staphylococcus aureus-induced excision wound in rats.     

Characteristics of Collagen from Rohu (Labeo rohita) Skin

Acid soluble collagen (ASC) and pepsin soluble collagen (PSC) were isolated from rohu skin with the yield of 64.2 and 6.8% (dry weight basis), respectively. Both collagens had glycine as the major amino acid with imino acid content of 196–202 residues/1,000 residues and were characterized as type I collagen with molecular composition of (α1)₂α2-heterotrimer. Fourier transform infrared spectra of both collagens were similar, with no shift in wavenumber of all amide bands. The T_max value of ASC and PSC was 36.40 and 35.48°C, respectively. The zero surface net charge of ASC and PSC was found at pH 5.9 and 5.3, respectively.     

Characteristics of a self-assembled fibrillar gel prepared from red stingray collagen

A translucent collagen gel was formed from a transparent acidic solution of red stingray collagen by adjusting to physiological ionic strength and pH in phosphate buffer and then incubating at 25–37°C. During fibril formation from red stingray collagen, the turbidity increased when the NaCl concentration was increased at constant pH and the rate of fibril formation was accelerated by higher pH or lower NaCl concentration. The T _m of red stingray collagen fibrillar gel was estimated as 44.3 ± 3.5°C, which was higher than that of the collagen solution, 33.2°C. In addition, red stingray collagen gel maintained its shape without melting and was suitable for culture of mouse stromal cells at 37°C.     

肾上腺髓质素对大鼠心肌成纤维细胞胶原合成的影响

目的:观察肾上腺髓质素(ADM)对大鼠心肌成纤维细胞(FBC)胶原合成的影响,并探讨其可能的作用机制。方法:用差速贴壁分离法获得心肌成纤维细胞为材料,用放射免疫法测定细胞培养上清液中I及III型前胶原末端肽(PINP、PCIII)的含量。结果:①ADM呈浓度依赖性抑制FBC的PINP、PCIII分泌;②在AngII存在的情况下,PKA抑制剂能完全阻断ADM抑制细胞PINP、PCIII分泌的作用;③在基础状态和AngII刺激下,PKC及酪氨酸蛋白激酶抑制剂均能增强ADM作用。结论;①ADM能够抑制FBC胶原合成;②ADM抑制FBC胶原合成是通过PKA途径介导。     

鲮鱼鱼皮胶原蛋白酶解物分子量分布与抗氧化性的研究(英文)

[目的]为了研究鲮鱼鱼皮胶原蛋白水解物的分子量的分布及抗氧化活性。[方法]分子量分布采用分子排阻色谱法和基质辅助激光解吸附飞行时间质谱(MALDI-TOF-MS),鱼皮用碱性蛋白酶2709处理。[结果]最佳水解条件为:pH 10.0、反应温度55℃、底物浓度为80 g/L、酶和底物比4%,水解时间3 h。两种方法分析水解产物的相对分子质量分布范围为400~1 800 Da,大多数胶原蛋白短肽的分子量在1 400 Da以下。[结论]结果表明,鱼皮水解物是一种潜在的抗氧化物。     

Intermedin inhibits pulmonary collagen synthesis in rats with pulmonary hypertension induced by high pulmonary blood flow

AIM: To explore the regulatory effect of intermedin (IMD) on pulmonary collagen synthesis and accumulation in rats with pulmonary hypertension induced by high pulmonary blood flow.METHODS: Healthy male SD rats (n=20) were randomly divided into control group (n=7), shunt group (n=7) and shunt with IMD group (n=6). The shunting of abdominal aorta and inferior vena cava was produced in rats of shunt group and shunt with IMD group. After 8 weeks, IMD was administered into the rats of shunt with IMD group subcutaneously by mini-osmotic pump for 2 weeks. Mean pulmonary artery pressure (mPAP), relative medial thickness (RMT) of pulmonary arteries, contents of hydroxyproline, collagen type I and III, bone morphogenetic protein-2 (BMP-2), and the mRNA expression of procollagen I and III in lung tissues were measured and compared. RESULTS: Compared with control group, mPAP and RMT of medium and small pulmonary arteries in the rats of shunt group were significantly increased. Meanwhile, the lung hydroxyproline, collagens I and III and BMP-2 contents, and the mRNA expression of lung procollagen I and III were all significantly increased compared with control group. However, IMD significantly decreased mPAP, alleviated the changes of pulmonary vascular micro-structure, decreased the collagen accumulation and pulmonary tissue homogenate BMP-2 contents, and inhibited the mRNA expression of procollagen I and III in the lung tissue of shunting rats.CONCLUSION: IMD plays a protective role in the development of pulmonary hypertension and pulmonary vascular structural remodeling induced by high blood flow by inhibiting pulmonary collagen synthesis and accumulation, possibly in association with the BMP-2 pathway.     

海参胶原蛋白的提取及其理化性质

胶原蛋白(PSC)是海参蛋白的主要组成,研究其理化性质为海参的进一步利用提供理论基础.以格皮氏海参为原料,采用胃蛋白酶促溶法提取海参胶原蛋白,并通过聚丙烯酰胺凝胶凝胶电泳、氨基酸分析以及傅立叶红外光谱对其理化性质进行研究.结果表明,海参胶原蛋白分子组成为(α1)3,α1的分子量约为150 kD;氨基酸组成以甘氨酸含量最高(6.01％),脯氨酸与羟脯氨酸含量为6.14％,符合水产胶原蛋白特征;傅立叶红外光谱分析结果表明其保持三螺旋结构.     

罗非鱼胶原蛋白多肽果味饮料的生产工艺及稳定性研究

[目的]确定罗非鱼胶原蛋白多肽果味饮料的最佳生产工艺。[方法]利用罗非鱼鱼鳞制成的胶原蛋白以及菠萝果汁为原材料,通过单因素度试验和正交试验来确定果味饮料的最佳配方和条件。[结果]罗非鱼胶原蛋白多肽果味饮料的最佳生产配方为菠萝果汁40%,蔗糖5%,有机酸0.4%,胶原蛋白5%;最佳稳定剂为黄原胶0.02%,单甘脂0.12%,海藻酸钠0.15%,阿拉伯胶0.10%;最佳均质压力为25 MPa;最佳杀菌温度为115℃时间为15 min。[结论]用罗非鱼鱼鳞和菠萝为原料制作的果味饮料营养丰富,稳定性好,生产工艺操作简单,具有较高的工业生产应用价值。     

Progressive Dermal Collagenosis of Male Miniature Swine

Abstract— A unique dermatosis of male miniature swine is described. The disease occurs in post-pubertal pigs, and is characterized by symmetrical, indurated, plaques over the truncal region. Histologically, the dermis and panniculus are effaced by thick, interwoven bundles of collagen resulting in an absence of deep dermal elastin. Hypertrophy and hyperplasia of superficial dermal vessels, fibroblasts and fibrocytes are accompanied by perivascular infiltrates of lymphocytes, plasma cells and eosinophils. The pathogenesis of this unusual dermatosis, designated as “progressive dermal collagenosis of male miniature swine” is unknown. Résumé— Une dermatose originale du cochon nain mâle est décrite. La maladie apparait chez des cochons post pubertaires, et est caractérisée par des plaques sur le tronc, indurées et symétriques. Histologliquement, le derme et le pannicule sont envahis par d‘épais falsceaux de collagène, entrainant l'absence d’élastine dermique. Les vaisseaux du derme superficiei sont hyperplasiés et hypertrophiés, des fibroblastes et des fibrocytes sont accompagnés d'un infiltrat inflammatoire périvasculaire de lymphocytes, éosinophiles et plasmocytes. La pathogénie de cette affection peu commune, appelée “collagénose dermique progressive du cochon nain mâle” est inconnue. Zusammenfassung— Es wird eine einzigartige Hauterkrankung beim männlichen Miniaturschwein beschrieben. Die Krankheit tritt bei postpubertären Schweinen auf und wird durch symmetrische, indurierte Plagues im Rumpfbereich gekennzeichnet. Histologisch treten Dermis und Pannikulus gegenüber dicken, verflochtenen Kollagenbündeln in den Hintergrund, wodurch das tiefe, dermale Elastin verschwindet. Die Hypertrophie und Hyperplasie der oberflächlichen Hautgefäße, Fibroblasten und Fibrozyten wird von perivaskulären Infiltraten aus Lymphozyten, Plasmazellen und eosinophilen Granulozyten begleitet. Die Pathogenese dieser ungewöhnlichen Dermatose, die als “progressive dermale Kollagenose des männlichen Miniaturschweins” bezeichnet wird, ist unbekannt. Resumen El presente artículo es una descripción de una dermatosis única del macho cerdo miniatura. Le enfermedad aparece después de la pubertad, y se caracteriza por la aparición de placas simétricas sobre la región del tronco. El exámen histológico de la dermis y del tejido panicular revela la presencia de gruesas bandas de colágeno entrelazadas, lo cual tiene como resultado la ausencia de la capa de elastina profunda. La hipertrofia e hiperplasia de los vasos superficiales dérmicos, fibroblastos y fibrocitos, se ve acompañadas de infiltrados perivasculares de linfocitos, células plasmáticas y eosinófilos. La patogénesis de esta rara dermatosis Ilamada ‘colagenosis dérmica progresiva del cardo macho miniatura’, es desconocida.     

Correlation between myocardial interstitial collagen remodeling and changes in hemodynamics in rats with myocardial infarction

AIM: To study the relationship between cardiac extracellular matrix remodeling and cardiac function after myocardial infarction. METHODS: We observed sequential changes in collagen contents and collagen Ⅰ/Ⅲ ratios in infarct zone (IZ) and non-infarct zone (NIZ) and their relationship to the parameters of left ventricular systolic and diastolic function in the rat model of myocardial infarction induced by ligation of left main coronary artery. RESULTS: Collagen conteants in IZ and NIZ after 3d of myocardial infarction were significantly higher than those in sham group at corresponding time (P<0.05, P<0.01). Collagen Ⅰ/Ⅲ ratio in IZ decreased on day 3, significantly increased after 7 d (P<0.01). Collagen Ⅰ/Ⅲ ratio in NIZ increased significantly afte14 d. Correlated analysis between collagen contents in IZ or NIZ and collagen type Ⅰ/Ⅲ ratio and maximal ascending velocity (+p'_max) or maximal descending velocity of the left ventricular pressure (-p'_max) was performed and the negative correlation between collagen contents in NIZ and +P'_max (r=-0.589, P>0.05) and -P'_max (r=-0.788, P<0.01) was found. Collagen content in IZ positively correlated to the +P'_max (r=0.70, P<0.50), but not to -P'_max (r=-0.29, P>0.05). Collagen type Ⅰ/Ⅲ ratios in NIZ correlated negatively to the +P'_max (r=-0.504, P>0.05) and -P'_max (r=-0.545, P>0.05), but there were no relationship between collagen type Ⅰ/Ⅲ ratios in IZ and +P'_max or -P'_max in IZ. CONCLUSION: Collagen deposition in IZ after myocardial infarction was of benefit to improvement of systolic function. Collagen deposition in NIZ was harmful to systolic and diastolic function.