Use of differently enriched rotifers,Brachionus plicatilis,during larviculture of haddock,Melanogrammus aeglefinus: effects on early growth,survival and body lipid composition

We evaluated the effects of enriched rotifers on growth, survival and on the lipid composition of haddock larvae. The treatments tested were (1) AlgaMac 2000^®, (2) AquaGrow^® Advantage and (3) Pavlova sp. paste and AlgaMac 2000^®. The treatments did not influence larval growth rate throughout the experimental period (P = 0.70). Larvae from all treatments grew approximately 8% of their dry weight per day between 1 and 29 days post hatch (dph). Treatment 3 resulted in the best survival, estimated to be 3 on a scale from 0 to 5, whereas for the two other groups the survival estimates were 0 and 2. Rotifers from treatment 1 had low sterol concentrations, high eicosapentaenoic acid/arachidonic acid ratio and their feeding resulted in high larval mortality. Rotifers enriched with Pavlova sp. had the lowest proportions of the sum of saturated fatty acids, docosahexaenoic acid and sum of ω3 and the highest proportions of the sum of monounsaturated fatty acids (ΣMUFA). This was partially reflected in larvae from treatment 3 in that they had the highest proportions of ΣMUFA and the lowest proportions of Σω3 (P < 0.0001 for both analyses). In addition, these larvae had the highest and lowest ΣC₂₀ and ΣC₂₂ polyunsaturated fatty acids (PUFA) respectively (P < 0.0001 for both analyses). We suggest that more research with ω3 and ω6 PUFA can lead to improvements in the rearing of haddock larvae produced in hatcheries.     

阳澄湖养蟹网围内外轮虫群落结构的变化及分析

2008年6月至2009年5月对阳澄湖养蟹网围及湖区3组对照点中轮虫群落结构及部分水质特征参数进行调查,分析了轮虫群落结构与环境变量的关系,利用多种参数对其水质进行评价。经鉴定,共采集到轮虫83种,隶属于12科30属。研究表明,网围内3个采样点的年平均生物密度及生物量低于湖区3个采样点,生物密度与水温呈极显著正相关(P<0.01)。不同月份受不同环境变量的影响,除4月份外,环境变量对生物密度的影响在采样点之间无差异。相似度分析表明,网围内及相邻湖区的轮虫优势种相似性高。水质分析表明,6个采样点均为富营养化、β-α中污染水体,东湖网围C₁和中湖网围A₁2采样点程度稍轻,但6个采样点之间无显著性差异,这与用卡尔森营养状态指数得出的结果相似。     

Mass Production of Freshwater Rotifer,Brachionus calyciflorus,Under Different Diets and Regimes

ABSTRACT

The production ability of freshwater rotifer, Brachionus calyciflorus, cultured with two algal species, Chlorella vulgaris, and Spirulina platensis, and a baker's yeast, Saccharomyces cerevisiae, were studied using semicontinuous culture method. B. calyciflorus was fed with the above three types of food at five different concentrations (125, 250, 500,750, and 1,000 μg/mL). Among the three different types of diet, maximum production of B. calyciflorus (489.20 ±10.91 individuals/mL; P < 0.05) was obtained with C. vulgaris, followed by S. cerevisiae, and S. platensis. Moreover, in each of the three diets, the maximum rotifer production was obtained at a particular concentration (C. vulgaris, 750 μg/mL; S. cerevisiae, 750 μg/mL; and S. platensis, 500 μg/mL) beyond which the rotifer production decreased. The peak production due to C. vulgaris (489 individuals/mL) was better than S. cerevisiae (321 individuals/mL) when the number of rotifers was considered. The present study indicates that the quantity and quality of food have a significant role on the rotifer production and that C. vulgaris at 750 μg/mL appears suitable to feed to rotifers for maximal production.     

轮虫培育池浮游生物的时空分布

在1995－1996年期间，对黑龙江、内蒙、辽宁3个地区11个池塘浮游生物的时空分布进行了研究。结果表明，浮游植物在池的上、下风位水平分布和表、底层垂直分布存在显著差异。轮虫的水平分布与风浪及水体溶氧状况有关，无风（或微风）时，上、下风位无明显差异。有风浪时，上风位比下风位高出1倍多。水体缺氧时，下风位比上风位高出4倍多，轮虫的垂直分布在溶氧充足时，上、下风位表层均低于底层；而在水体缺氧时，上、风位表层高出底层近3倍。桡足类的水平分布上风位比下风位高出2－4倍，垂直分布无明显差异。文中还讨论了轮虫池浮游生物分布的某些规律和成因及其在生产实践中的意义。     

A comparison among differently enriched rotifers (Brachionus plicatilis) and their effect on Atlantic cod (Gadus morhua) larvae early growth, survival and lipid composition

We evaluated the effect of differently enriched rotifers on the early growth, survival and lipid composition of Atlantic cod larvae (Gadus morhua). The enrichments tested were: (i) AlgaMac 2000^®; (ii) AquaGrow^® Advantage; and (iii) a combination of Pavlova sp. paste and AlgaMac 2000^®. Larvae from treatment 3 [1.50 ± 0.11 mg dry weight (dw) and 7.10 ± 0.14 dw specific growth rate (SGR)] were heavier (P = 0.006) and grew faster (P = 0.004) than larvae from treatment 2 (1.03 ± 0.04 mg dw and 6.29 ± 0.04 dw SGR). No significant differences were found in the final weight and SGR among larvae from treatment 1 (1.21 ± 0.07 mg dw and 6.58 ± 0.20 dw SGR) and larvae from treatments 2 and 3. The treatment 3 also resulted in the best survival at the end of the experimental period, estimated to be 3 on a scale from 1 to 5, whereas the survival estimates for the two other groups were 1–2. Larvae from the treatment 3 reached 37 days posthatch with levels of ω6DPA 32‐fold higher than newly hatched larvae. Differences in the larval enrichment of ω6DPA may explain the differences in growth and survival of the Atlantic cod larvae.     

Effects of docosahexaenoic acid on growth,survival and swim bladder inflation of larval amberjack ( Seriola dumerili, Risso)

The effect of docosahexaenoic acid (DHA) on the growth performance, survival and swim bladder inflation of larval Seriola dumerili during the rotifer feeding period was investigated in two feeding experiments. Amberjack larvae at 3 day post hatching were fed rotifers enriched with (1) freshwater C hlorella (Chlo), (2) a mixture (2:1, v/v) of Chlo and DHA‐enriched C hlorella (DHA‐Chlo), (3) DHA‐Chlo and (4) DHA‐Chlo and commercial DHA emulsion, in triplicate for 7 days. The average DHA contents of the rotifers were 0.0, 0.4, 1.0 and 1.9 mg g^?1 DM respectively. The survival rate was improved by the enrichment of rotifers with DHA‐Chlo alone, and DHA‐Chlo and emulsion. Growth and swim bladder inflation of fish fed rotifers enriched with DHA‐Chlo were significantly (P < 0.05) improved, however, with increased levels of DHA further improvement was not found. DHA content in the larval whole body proportionally increased with the DHA level in the rotifers. These results suggest that DHA enrichment of rotifers is effective to improve the growth, survival rate and swim bladder inflation of amberjack larvae. The DHA requirement of amberjack larvae is estimated to be 1.5 mg g^?1 on a dry matter basis of rotifers.     

轮虫石蜡切片制作方法的改进

轮虫个体微小、组织柔软,对外界环境刺激反应较强烈,同时体表覆盖有角质层,采用常规的石蜡切片技术,难以切出完整的切片。通过对传统制片技术的改进,可以使标本接近自然状态,取得了比较理想的效果。     

Use of flubendazole as a therapeutic agent against rotifers (Brachionus plicatilis) in intensive cultures of the harpacticoid copepod Tisbe holothuriae

Copepods are well known to be the optimal live feed for most species of marine fish larvae. Still copepods are rarely used in marine hatcheries worldwide. Lack of efficient production techniques are among the reasons for this. Consequently, Artemia and rotifers are utilized in commercial settings. One problem in intensive production of copepods is contamination with rotifers. Rotifers have higher growth rates than copepods and consequently will compete out the copepods when accidentally introduced to the copepod production systems. Once contamination has occurred, the only cure has been to shut down production and subsequently use a therapeutic agent to eliminate all zooplankton in the system before restart with a stock culture free of rotifers. We tested flubendazole as a mean of controlling rotifers (Brachionus plicatilis) in intensive laboratory cultures of the harpacticoid copepod (Tisbe holothuria). Flubendazole was lethal to rotifers in concentrations as low as 0.05 mg L^?1. There was no significant effect on the concentration of copepods, even at the highest concentration tested, i.e. 5.0 mg L^?1 flubendazole. We conclude that flubendazole is an effective drug for control of B. plicatilis in T. holothuriae batch cultures.