Macro and Micro Mineral Levels in the Tissues of Menhaden Fish

Various tissues of menhaden fish were analyzed for macro and micro minerals. Tissues selected were gill, heart, intestine, kidney, liver, muscle, spleen, and stomach. Phosphorus, calcium, magnesium, sodium, manganese, and zinc were found to be most concentrated in the gill. Potassium, iron, and boron were least concentrated in the gill. Liver contained the highest concentration of copper, selenium and boron. The stomach was the highest in potassium and lowest in phosphorus. Sodium and selenium concentrations were lowest in the muscle. Mercury concentrations were approximately 2 mg/g dry matter in all tissues     

广东对虾产业竞争力分析

经过30多年的发展，广东已经成为中国最大的对虾生产和出口地区。广东既具有对虾生产和出口方面的绝对优势，又具有资源和竞争力等方面的相对比较优势。面对国内外同业的竞争，增加高附加值产品、促进出口市场多元化是增强广东对虾竞争优势的关键。     

Utilization of monounsaturated fatty acid (18:1n-9, oleic acid) by freshwater prawn Macrobrachium rosenbergii (de Man) juveniles

The utilization of oleic acid as an energy source and the effects of oleic acid levels and/or dietary soy bean lecithin (SBL) on oleic acid utilization, growth and survival, and lipid class and fatty acid compositions of freshwater prawn, Macrobrachium rosenbergii (de Man), juveniles were determined.
Increase in levels of dietary oleic acid from 10 to 80 g kg⁻¹ significantly ( P ≤ 0.05) reduced growth, survival and feed conversion efficiency of M. rosenbergii juveniles during the 40-day feeding period. Inclusion of 20 g kg⁻¹ SBL had no significant effect ( P ≥ 0.05) on growth and survival, nor was there any interactive effect between dietary SBL and oleic acid levels.
Body fatty acid profile of prawns reflected dietary fatty acid quantity. The fatty acid composition of prawns fed diets containing 80 g kg⁻¹ oleic acid had tremendously high proportions of oleic acid. Polar lipids, mostly phosphatidylcholine (PC), constituted the bulk of the extracted total lipids. Prawns fed with SBL had significantly ( P ≥ 0.05) higher PC content.
Oleic acid was metabolized for energy by M. rosenbergii juveniles at the same rate regardless of dietary level of SBL and/or oleic acid. Expired ¹⁴CO₂ accounted for half of the ingested radioactivity 48 h after feeding with labelled diets. No significant difference in the amount of ¹⁴CO₂ expired by prawns fed the labelled test diets was found. Per cent radioactivity ingested and absorbed into the body was also not significantly different in prawns of the different dietary treatments.     

鲈与中国对虾混养试验

１９９５、１９９６年进行鲈与中国对虾混养试验，利用鲈摄食病虾及小型糠虾类，阻断病毒传播，推迟了对虾发病，试验池产量，效益成活率均高于对照池。     

靖海湾和五垒岛湾放流增殖中国对虾的回游与分布

用烧结法标志中国对虾，在靖海湾和五垒岛湾进行增殖放流，研究其回游与分布规律。重捕资料表明，６月中旬幼对虾对湾内河口浅水区生活随体长增长，逐步向深水扩散；８月上，中旬主群陆续游向湾外，按地形走向向西南回游，部分向东或东南；１０月份回游对湾外水域的对虾有向东，东北或东南回游的现象。     

Gene Expression in Black Tiger Prawns Following Intramuscular Injection of β-gal Plasmid

Gene expression in black tiger prawns (Penaeus monodon) was studied following intra-muscular injection of CMVGal plasmid into the second abdominal segment. We used an in situ staining technique to detect -gal expression in one- and three-month-old injected prawns. We found that only one of the three-month-old prawns expressed the marker gene (2 days after injection), and the site of expression was confined to the sixth abdominal segment away from the injection site. We repeated the experiment on a new batch of three-month-old prawns, but using fluorometric determination technique. This time we found that -gal expression was detected (6/42) at the site of injection after 2, 7, and 14 days. In two other test samples, transgene expression was detected in the sixth abdominal segment only, further confirming the possibility of injected DNA dispersal. The results of the study also suggest that direct gene transfer is a feasible technique in black tiger prawns.     

中国对虾育苗中几项新的技术措施

本文针对目前中国对虾苗种生产中幼体病害日益严重的问题，提出了采用卵子消毒和生态防病作为预防疾病的重要手段，并介绍了几种消毒剂用于对虾幼体不同时期的安全浓度，为对虾育苗生产中防治病害提供了几项新的技术措施。     

转盘式对虾开背工艺分析与参数优化

针对目前对虾开背环节工艺不完善、实际应用装备缺乏等问题,以中型南美白对虾为研究对象,探究了对虾开背关键工艺方法,建立了对虾开背过程力学模型,获得影响开背效果主要因素为刀具角度、加工中心盘转速和刀具安置距离;采用单因素试验研究和分析了各因素对开背成功率、虾仁损伤率、虾线裸露成功率和感官评分值的影响,确定影响因素最佳区间为：刀具角度20°~60°、加工中心盘转速10~40r/min和刀具安置距离9~11mm;利用Design-Expert软件进行多因素响应面试验研究分析,并采用多目标优化方法,得到最优参数组合为：刀具角度43.838°、加工中心盘转速28.391r/min、刀具安置距离9.801mm时,开背成功率为99.161%、虾仁损伤率为2.825%、虾线裸露成功率为90.727%、感官评分值为86.944。验证试验结果表明：针对中型南美白对虾,当刀具角度45°、加工中心盘转速28r/min、刀具安置距离9.8mm时,开背成功率为98.89%、虾仁损伤率为3.33%、虾线裸露成功率为87.78%、感官评分值为85.33,与理论优化值的绝对误差均较小,优化后的对虾开背装置性能满足作业要求。研究可为对虾开背工序装置设计和参数选择提供理论基础和科学依据。