Dietary threonine requirement of fingerling Indian major carp,Labeo rohita (Hamilton)

An 8‐week feeding experiment was conducted in a water flow‐through system (26–28 °C) to determine the dietary threonine requirement of fingerling Labeo rohita (3.90±0.03 cm; 0.58±0.02 g). Growth, feed utilization and body composition of fish fed test diets (40% crude protein; 17.9 kJ g^?1 gross energy) with graded levels of l ‐threonine (0.75%, 1.0%, 1.25%, 1.50%, 1.75% and 2.0% dry diet) to apparent satiation were response variables used to assess threonine adequacy. Diets were made isonitrogenous and isoenergetic by adjusting the levels of glycine and dextrin. The amino acid profiles of the test diets were formulated to that of 40% whole chicken egg protein except for threonine. The performance of fish fed experimental diets was evaluated using calculated values for weight gain (g fish^?1), feed conversion ratio (FCR), protein efficiency ratio (PER) and protein productive value (PPV) data. Maximum weight gain (g fish^?1) (1.79), lowest FCR (1.39), highest PER (1.76) and PPV (0.33) were recorded at 1.50 g per 100 g dietary threonine. Statistical analysis of weight gain, FCR, PER and PPV data reflected significant differences (P<0.05) among treatments. Except for reduced growth performance in fish fed threonine‐deficient diets, no deficiency signs were noted. Weight gain, FCR, PER and PPV data were also analysed using second‐degree polynomial regression analysis to obtain a more accurate threonine requirement estimate, which was found, using each response variable, to be at 1.70, 1.63, 1.65 and 1.51 g per 100 g of dry diet, corresponding to 4.2, 4.07, 4.12 and 3.77 g per 100 g of dietary protein respectively. Based on the second‐degree polynomial regression analysis of the live weight gain, FCR, PER and PPV data, the optimum dietary level of threonine for fingerling L. rohita was found to be in the range of 1.51–1.70 g per 100 g of the dry diet, corresponding to 3.77–4.2 g per 100 g of dietary protein.     

乌鳢苗种繁育及池塘高产养殖技术

为探讨乌鳢的人工养殖技术,对乌鳢苗种繁育及池塘养殖阶段的关键环节:亲鱼培育、人工催产、鱼卵孵化、鱼种培育、成鱼养殖等进行了系统研究。并总结了一套实用的乌鳢养殖技术。实践证明,使用该技术能提高乌鳢鱼苗成活率和成鱼产量。     

罗非鱼鱼种肠炎病的病理组织学及病原体的研究

本文从病理组织学的角度,研究了罗非鱼鱼种肠炎病的发生与发展,为其诊断与防治奠定了理论基础。结果表明,该病病原体为产气单胞菌。     

赤眼鳟人工繁殖与苗种培育技术

试验进行了野生赤眼鳟（Squaliobarbus curriculus）的人工驯养、繁殖及苗种培育,并对其受精卵的胚胎发育情况进行了观察,结果表明：单独使用或组合使用促黄体素释放激素类似物（LRH-A2）、马来酸地欧酮（DOM）,以胸腹注射法进行一针或两针人工催产,效果都较好,效应时间一般随水温升高而缩短,24℃～27.5℃一针注射后的效应时间为10～12 h;22℃～23℃两针注射（距第二针）的效应时间为8h左右;浮盘与水泥池组合式孵化方式合理、可靠,3年累计孵出鱼苗218.2万尾;赤眼鳟的孵化时间与水温呈负相关,水温越高,孵化时间越短,在21～24℃时,一般需32 h孵出,而当水温上升至24～27.5℃时,则仅需20～24h即可出膜;鱼种培育阶段,生长较快,冬片鱼种规格也能达到10 cm～15 cm,3年累计育出赤眼鳟冬片鱼种22.81万尾,平均成活率达82.95%.     

池塘主养彭泽鲫鱼种高产试验报告

池塘面积1067m^3，主养彭泽鲫鱼种，搭配团头鲂及鲢鳙鱼种，经156天饲养，共产鱼1029．6kg，折合667m^2产鱼643．5kg。其中每667m^2产彭泽鲫鱼种367．8kg,平均规格70g。每667m^2利润3498元。投入产出比为1：1．67。     

杂交鲶(怀头鲶♀×鲶♂)人工繁殖与苗种培育技术

采用捕自黑龙江水域的怀头鲶为母本，鲶鱼为父本，应用自制催产药物DOS与LRH-A2、HCG进行人工催产，使催产率、受精率分别达到92％和88％。20-21．5℃水温条件下43h孵化出苗，孵化率75％。出膜仔鱼体长3．93mm，7．83mm下塘，池塘培育8天达到34．08mm，培育成活率50％。     

黄颡鱼的生物学特性与养殖技术

通过对黄颡鱼的生物学特性、繁殖技术、苗种培育技术、养殖技术、放养模式、病害防治技术的系统研究，探明了黄颡鱼的食性、溶氧需求、温度适应性、盐度耐受性等生物学特性，报道了黄颡鱼繁殖与苗种培育措施，提出池塘养殖所需配套技术及常见病害防治技术。     

草鱼种与泥鳅池塘双主养模式养殖试验

[目的]降低草鱼池塘养殖生产成本,提高池塘水体利用率和综合养殖效益,增强养殖模式的抗风险能力以及示范推广作用.[方法]在江苏省宿迁市宿城区中扬镇开展了草苗种、泥鳅池塘双主养模式养殖试验.[结果]放养260尾/kg的泥鳅苗种180 000尾/hm2,放养2 000尾/kg的草苗种60 000尾/hm2的养殖模式,以及放养260尾/kg的泥鳅苗种120 000尾/hm2,放养2 000尾/kg的草苗种75 000尾/hm2的养殖模式,产出投入比均较高.[结论]放养260尾/kg的泥鳅苗种120 000尾/hm2,放养2 000尾/kg的草苗种75 000尾/hm2的养殖模式更容易被养殖者接受,更容易被大面积推广应用.     

对草鱼、鲢、鳙、团头鲂鱼种阶段的RNA／DNA的初步研究

本文对草鱼、鲢、鳙、团头鲂4种养殖鱼类在鱼苗期、1龄期、2龄期的RNA和DNA含量进行测定．结果指出RNA／DNA的变化规律与鱼类的生长情况一致，因此，RNA／DNA可以怍为评定这4种养殖鱼类在鱼种阶段生长的指标。     

Effect of dietary supplementation with taurine, β-alanine andGABA on the growth of juvenile and fingerling Japanese flounder Paralichthysolivaceus

ABSTRACT:   Three diets supplemented with taurine, β-alanine andGABA and a control diet were fed to juvenile and fingerling Japaneseflounder to investigate the effects of the diets on growth and metabolicchanges of free amino acids in whole body and tissues. In experimentI, three diets supplemented with 1% each of taurine, β-alanineand GABA and a control diet were fed to juvenile Japanese flounderwith an initial mean body weight of 0.4 g for 4 weeks at20°C. In experiment II, the taurine-supplemented diet anda control diet were fed to fingerling Japanese flounder with an initialmean body weight of 15 g for 4 weeks at 20°C.Only supplementation of taurine in the diet of juvenile flounderimproved their growth performance in experiment I, but fingerlinggrowth performance of experiment II was not significantly relatedto taurine supplementation in the experimental diet. These resultssuggest that there is a greater requirement for taurine for thegrowth of juvenile Japanese flounder than fingerling Japanese flounder.