Mycobacteriosis caused by Mycobacterium marinum in reared mullets: first evidence from Sardinia (Italy)

Mycobacterium marinum is a slow‐growing non‐tuberculous mycobacterium, and it is considered the most common aetiologic agent of mycobacteriosis in wild and cultured fish. The diagnosis is principally made by histology when positive Ziehl–Neelsen stain granulomas are detected. The aim of this study was to investigate the occurrence of mycobacteriosis in extensively cultured Mugilidae of two lagoons (Cabras and San Teodoro) from Sardinia by the use of histology, microbiology, PCR and DNA sequencing. Nine of 106 mullets examined were affected by mycobacteriosis, and the spleen was the most affected organ. The histology detected higher rate (100%) of infection in spleen than the culture and PCR (75% and 62.5%, respectively). The sequencing of hsp65 gene identified M. marinum as the primary cause of mycobacteriosis in the mullets examined. Mullets affected by mycobacteriosis were mainly fished in the San Teodoro lagoon characterized by critical environmental conditions. Histology remains the most common method in detecting fish affected by mycobacteriosis, and PCR‐based methods are essential for species identification. Our finding are worthy of attention because mycobacteriosis caused by M. marinum in reared mullets was evidenced for the first time in Sardinia, suggesting that this disease may be underestimated also in other cultured fish species.     

Getting cosy in freshwater: Assumed to be brackish pike are not so brackish after all

Pike (Esox lucius) occupy coastal streams and rivers of the Baltic Sea, where they attain large sizes (>5 kg). These large sizes are perhaps due to the fact that they can tolerate relatively high salinities and can thus forage in the nearby more productive brackish environments. In an attempt to quantify the extent to which pike utilise brackish environments, and to provide some insight into the underlying causes for brackish water migrations, we tagged 30 pike from a western Baltic river with acoustic transmitters and were able to track 21 individuals for 1 year. Based on experienced from local anglers, this population was assumed to be brackish in nature, where individuals underwent freshwater migrations to spawn. Our findings however suggest that the smallest and most active individuals make short exits into brackish waters and do so on rare occasions. Our results further indicate that neither sex nor size is related to activity level. We suggest that these patterns reflect two distinct behaviours—active and passive—and that large pike can be supported by the food availability in the river, without the need to venture into coastal zones, thus defying the conventional view that Baltic pike are all brackish in nature.     

微咸水膜下滴灌对土壤水盐分布及加工番茄产量的影响

为探明微咸水膜下滴灌对土壤水盐分布及加工番茄生长和产量的影响,通过大田小区试验,设置灌水矿化度和灌水定额两个因素,其中3个灌溉水矿化度水平分别为S1:1 g·L~(-1)、S2:3 g·L~(-1)和S3:5 g·L~(-1),3个灌水定额分别为W1:305 m~3·hm~(-2)、W2:458 m~3·hm~(-2)和W3:611 m~3·hm~(-2),来进一步寻求适宜本地区加工番茄生长的微咸水膜下滴灌灌溉制度。结果表明:覆膜微咸水滴灌条件下土壤含水量垂直方向的变化趋势表现为0～20 cm土层随深度增加含水量逐渐降低、20～100 cm土层随深度增加含水量逐渐增大、60～100 cm范围内土层剖面含水量最大的分布规律;土壤含盐量随着灌水矿化度的增大而增加,且随着灌水量的增加土壤盐分逐渐向水平距滴灌带35 cm处聚集。灌水矿化度超过3 g·L~(-1)时加工番茄株高、茎粗均受到一定程度的抑制作用,但对产量影响不大。本文通过试验得出:灌水定额为611 m~3·hm~(-2)、矿化度为1 g·L~(-1)处理为本地区最佳微咸水膜下滴灌处理,加工番茄生长健壮且产量最高,达到127 613.2 kg·hm~(-2);同时认为,在我国淡水资源比较缺乏的新疆地区可以考虑采用灌水定额458 m~3·hm~(-2)和灌水矿化度3～5 g·L~(-1)的微咸水对盐分中等敏感的加工番茄进行灌溉。     

盐渍土区微咸水灌溉对冬小麦的影响

河北省黑龙港地区储有相对丰富的浅层地下微咸水。为合理开发微咸水资源，研究了不同灌水（灌水期、灌水量和水质）对冬小麦生长发育和产量的影响。结果表明：咸淡轮灌与灌淡水相比，早期干物质积累慢，LAI低，但后期积累养分能顺利转移到穗部，灌浆速度较快，也能获得高产。冬后关键期灌水，咸淡轮灌比灌淡水处理LAI较低，但茎叶干物质积累量较多的转移到籽粒中，灌浆速度快，两者籽粒产量无显著差异。关键期灌水的籽粒产量略低于充足灌水处理，而水分利用率较高。在此基础上，施肥处理的LAI、灌浆速度和籽粒产量显著大于不施肥处理。     

微咸水造墒对棉花生长发育及产量的影响研究

采用小区对比试验方法,研究了河北低平原区旱地等雨播种（HD）、播前淡水造墒（S1）以及矿化度为2.2g/L（S2.2）和5g/L（S5）微咸水造墒等不同处理对棉花生长发育和产量的影响。结果表明,棉花生育期内HD处理0～100cm土壤含水率明显低于3种造墒处理;S5处理0～40cm土壤盐分含量最高,但降雨淋洗效果较为显著...     

微咸水和再生水对盆栽棉花土壤理化性质和根系的影响

为缓解华北水资源短缺问题,寻求代替水源,进行了微咸水和再生水灌溉条件下不同灌水量对盆栽棉花土壤理化性质和棉花根系影响的试验。试验设置灌水水质和灌水量2个试验因素,灌水水质设计为低、中、高矿化度微咸水和再生水,清水作为对照;灌水量设计为田间持水量的95%、85%、70%、55%。试验结果表明:微咸水和再生水处理增加土壤含盐量,微咸水处理的土壤含盐量随着矿化度的升高而增加;再生水处理提高土壤有机质和硝态氮的含量;微咸水和再生水促进棉花根系的生长,在盐分累积严重的情况下根系生长最快。     

磁化或电离化微咸水理化特性试验

改善微咸水理化特性,提高微咸水利用效率,成为合理利用微咸水重要研究内容。为了对微咸水活化效果及活化微咸水理化特性进行定量评价,该文采用磁化和电离化法对微咸水进行不同的活化处理,通过室内测试,对电离化微咸水活化系统的技术参数进行了分析,并对活化微咸水的表面张力系数、溶氧量、电导率及p H值等理化特性进行了研究。结果表明,电离化系统要求接地电阻不超过5Ω;活化微咸水表面张力系数显著减小、溶氧量显著增加、电导率和p H值受到不同程度的影响;但对表面张力系数和溶解氧影响比较明显,对电导率和p H值影响不显著。在矿化度5 g/L时,活化微咸水表面张力相对减少9.14%~13.84%,溶氧量相对增加8.04%~10.23%,表面张力相对减少量与溶氧量相对增加量可以从客观上反应活化微咸水理化特性的变化,而表面张力相对减少量与溶氧量相对增加量之间又存在显著的指数函数关系。根据表面张力相对减少量,电离化微咸水活化处理效果优于磁化处理,磁场强度3 000 Gs与变频磁化处理活化效果显著。因此,可以用表面张力相对减少量作为定量评价指标对活化微咸水的理化特性进行综合性定量评价,以判断不同活化方式的活化效果。     

秸秆排水体埋深对盐渍土水盐分布的影响及排水抑盐效果

为探究秸秆排水体对盐渍土水盐运移的影响及其排水排盐效果,通过室内土柱试验,研究在淡水(CK)和微咸水灌水情况下秸秆排水体埋深为40和60 cm时供试土壤的水盐分布状况。结果表明,入渗阶段,湿润锋与入渗时间呈幂函数关系,累积入渗量与入渗时间则可采用Kostiakov模型进行拟合;蒸发阶段,秸秆排水体对埋设深度以下的土体具有明显的保水作用,40 cm埋深的处理在40~70 cm土层范围以及60 cm埋深的处理在50~70 cm土层范围土壤水分变化的相对变化量均0;秸秆排水体有利于保持灌水后土壤的脱盐状态,40 cm埋深处理和60 cm埋深处理比无埋设的对照处理分别减少了19.61%和15.68%的盐分变化量;秸秆体的排水排盐效果与灌水矿化度和秸秆排水体埋深密切相关,灌溉水矿化度适当的增加和秸秆体的埋设加深将有利于排水效果的提升,低灌溉水矿化度结合秸秆体深埋具有更好的排盐效果。该研究为微咸水灌溉及盐渍土的开发利用提供依据和参考。     

基于SALTMOD模型的灌溉水矿化度对土壤盐分的影响

以河套灌区沙壕渠灌域为例,运用SALTMOD模型探讨了区域尺度灌溉水矿化度对根层土壤盐分的影响。结果表明,根层土壤盐分随灌溉水矿化度的增大而增加,加大排水沟深度和提高渠道衬砌水平可缓解高矿化度灌溉水对土壤积盐的影响;采用地下微咸水和黄河水混合灌溉可有效控制盐渍化的发展,混合比在1∶1范围内时,根层土壤处于脱盐状态,最高脱盐率为23%,脱盐率高于引用黄河水灌溉的脱盐率(4%)。因此,适度利用地下微咸水灌溉,可有效的控制地下水位,节约淡水资源。     

Single nucleotide polymorphisms found in the red alga Furcellaria lumbricalis (Gigartinales): new markers for population and conservation genetic analyses

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