几种二氮氧化喹恶啉甲醛新衍生物的合成及其抑菌活性的研究

合成了5种二氮氧化喹恶啉甲醛的新衍生物并用HNMR谱和元素分析对其结构进行了表征。用新的化合物对5种常见的植物病菌进行了抑菌活性并进行了抑菌活性和结构关系的初步探讨。     

不同抗生素对生姜组织培养中姜芽脱菌效果的研究

以PDA和Plate Count Agar作为基本培养基,外加不同浓度配比抗菌素进行脱菌。结果表明：以PDA125 mL作为基本培养基外加1 g/L黄连素10 mL、1 g/L卡那霉素10 mL和外加1 g/L黄连素10 mL、1 g/L多菌灵10 mL,姜块脱菌试验染菌率为0,脱菌效果良好。以PDA125 mL作为基本培养基外加1 g/L青霉素10 mL、1 g/L卡那霉素10 mL,姜芽脱菌试验染菌率为0,脱菌效果良好,另外整个试验过程姜芽的脱菌效果均优于姜块的脱菌效果。     

The effect of topical minoxidil treatment on hair follicular growth of neonatal hairless descendants of Mexican hairless dogs

The effect of daily topical minoxidil treatment on hair growth was investigated in eight neonatal hairless pups. After 21 days of minoxidil treatment, hair growth occurred in the minoxidil-treated areas of each neonatal hairless pup. The numbers of hairs in the treated areas increased as compared with that in the areas treated with vehicle only. Histological findings revealed that the skin treated with minoxidil contained many hair follicles derived from epidermal ingrowths (hair germs) projecting into the dermis. These hair follicles showed various stages of neofolliculogenesis. After 31 days of treatment, terminal hair growth was observed. In the minoxidil-untreated areas, epidermal ingrowths remained undifferentiated. These results revealed that hairless dogs developed hair growth reactions following minoxidil-treatment when such treatment is applied in the early neonatal period. Neonatal hairless pups are a useful model for evaluating the effectiveness of hair growth stimulators.     

带菌豇豆种子包衣效果试验

采用 6种不同种衣剂对带菌的豇豆种子进行包衣育苗及直播栽培试验。结果表明 :种衣剂不仅提高了豇豆的出苗率 ,促进了幼苗的生长 ,而且对豇豆种子本身所带的炭疽病和土传的猝倒病、枯萎病均有明显的防效。从综合效果看 ,合肥丰乐营养包衣剂和ZBS菜豆包衣剂较其他种衣剂效果更好     

广西上思县桉树青枯病原菌致病性的研究

[目的]为林业生产防治青枯病提供理论指导。[方法]从广西上思县2种发病症状的桉树病株根上收集青枯病病原菌,按柯赫原则研究其致病性。[结果]2种症状的90%以上分离物的菌落形态完全相同,分离培养证实均是与自然界完全相同的青枯病菌。人工培养基上培养的慢性型青枯病菌部分菌株发生变异,致病性减弱甚至丧失,急性型青枯病菌的菌株致病性未减弱。接种试验表明,急性型症状病株上分离所得青枯病病原菌致病力强于慢性型症状的病原菌,桉树苗木死亡率高,发病时间早,发病程度也有一定差异。[结论]2种症状分离到的病原菌都为青枯病菌,均有明显致病性,急性型病原菌致病力强于慢性型,筛选出4个致病力较强的菌株。     

荷斯坦牛隐性乳房炎主要病原菌的耐药性试验

对新疆石河子市某奶牛场进口的澳大利亚荷斯坦牛隐性乳房炎的发病情况进行调查，同时采集部分乳样进行细菌的分离鉴定，结果发现其中主要致病菌为链球菌、金黄色葡萄球菌、大肠杆菌等，并分别选取了15种常用抗生素进行了药物敏感试验。结果表明：致病菌对临床上常用的青、链霉素及磺胺类等药物均已产生不同程度的耐药性,而喹诺酮类药物则相对表现出较好的抑菌效果。     

氯化汞处理带病菌小麦种子对正常幼苗率效应的研究

针对种子检验工作中存在的带病菌作物种子影响正常幼苗率的问题，选择出适当药剂氯化汞，采用二次回归正交设计的统计分析方法，对带病菌的小麦种子进行了研究，结果表明：没处理的种子由于发芽时100％长有霉菌，正常幼苗率只有40％，而此批种子经生活力测定结果为生活力64％。经筛选出的氯化汞最适处理组合（浓度0．128％，处理7min41s),可使种子正常幼苗率达最高为63％，基本和生活力相符。此外，本研究应用的统计分析的实例对今后的农业试验研究也有一定的借鉴和指导作用。     

光合细菌在种植业上的应用研究

把光合细菌作为有机肥施人烟草、萝卜、大豆、地瓜等试验田中，结果显示：光合细菌能改善土壤微生物区系，促进土壤中固氮菌、根瘤菌、放线菌、细菌等的生长，抑制土壤中真菌的生长；能增加大豆根瘤数；能促进作物对土壤中各养分的吸收，增加植株叶绿素含量，促进植物生长，使各植株的株高、根长、根重等都比对照有显著增加；光合细菌还能增加作物产量，改善作物品质，尤其对地瓜的产量增加明显，其增幅达78．5％，有一定的推广价值。     

枯草芽孢杆菌B47高产拮抗物质菌株的紫外诱变选育(英文)

[Objective] The aim of this study was to breed new strains which have higher inhibitory effects on the pathogens of watermelon fusarium wilt.[Method] The endophytic Bacillus subtilis B47 strain was obtained from tomato stems by UV mutagenesis for two consecutive times,then genetic stability as well as physiological and biochemical properties of mutant strains were studied.[Result] The antibacterial activity of all the three mutant strains F303,F304 and F305 was higher than that of B74 strain.After subculture of 10 successive generations,the antibacterial activity of all the three mutant strains for the pathogens of watermelon fusarium wilt decreased,but the antibacterial activity of F305 strain decreased the least,indicating its best genetic stability among the tested strains.The antibacterial circle diameter of F305 strain was 5 mm larger than that of wild strain B47 under the same condition.The mutant strain F305 was in logarithmic growth phase within 36 h and in stationary phase within 36-96 h,while its optimum growth temperature was 35 ℃.F305 strain could grow in sodium salt with the concentration of 1%-10%,but it grew best at the concentration of 1%.Physiological and biochemical responses of F305 strain were in accordance with those of wild strain B47.[Conclusion] This study lays the foundation for the factorial production of antagonistic substance by B47 strain and new methods of preventing from the pathogens watermelon fusarium wilt.