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China's alfalfa market and imports: Development,trends, and potential impacts of the U.S.–China trade dispute and retaliations 下载免费PDF全文
This study examines the development and trends of China's alfalfa market and imports, identifies key factors for the rapid increase in China's alfalfa imports, and discusses potential impacts of the U.S.–China trade dispute and retaliations on the alfalfa markets and trade in both nations. China's rapid transition toward larger-scale commercial dairy production, with enhanced feed and cost management as well as quality and safety control, and its limited resources for high-quality alfalfa production are key factors for the dramatic increase in its alfalfa imports, from 19 601 metric tons in 2008 to 1.38 million metric tons (mmt) in 2018. While the United States dominated China's alfalfa imports with an average share of 97.01% from 2007 to 2017, the share dropped to 83.76% in 2018 and 63.28% in January 2019 due to the trade dispute and retaliations started in 2018. China will likely remain a large importer of alfalfa because of both its growing demand and the comparative advantages of imported alfalfa in quality and price, but the imports from the United States will be highly affected by the ongoing trade dispute and negotiations. China is also expected to make more efforts to reduce its dependence on U.S. alfalfa through increased investment in domestic alfalfa production and identification of alternative sources of alfalfa and other hay imports. 相似文献
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屈芳 《农业图书情报学刊》2014,(9):90-92
U阅迷你书房是顺应时代为解决公共图书馆数字信息平台缺陷的创新产品。文章就U阅迷你书房自身定义的特点、与传统图书书籍的区别和对未来图书馆所造成的影响趋势这三个方面,从不同的角度详细论述了U阅迷你书房的优势及在未来被推广的必然性,从而阐明U阅迷你书房是公共图书馆发展所必不可缺的。 相似文献
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[目的]获得有效的水牛RNA聚合酶Ⅲ启动子序列,为开展水牛源细胞的基因特异沉默研究奠定基础.[方法]通过启动子上、下游保守序列对水牛源启动子7SK、U6进行克隆和启动子关键顺式作用元件识别,利用一段针对EGFP的shRNA片段(shEGFP)对水牛7SK、U6启动子进行功能性分析,然后分别在水牛源及鼠源细胞中与pEGFP-N1共转染,转染48h后用荧光显微镜检测EGFP的表达情况,并用流式细胞仪和荧光实时定量PCR检测EGFP沉默表达情况.[结果]克隆获得水牛7SK、U6启动子序列分别为430和357 bp,其OCT-1(或CACCC盒)及TATA盒高度保守.连接shEGFP后与pEGFP-N1共转染细胞,通过荧光显微镜可观察到细胞发生了明显的荧光表达沉默现象;通过流式细胞分析,发现水牛7SK和U6启动子引导的shEGFP在水牛源细胞中沉默效率高达93.82%和87.45%;荧光实时定量PCR检测结果显示,在转染bu7SK-shEGFP和buU6-shEGFP的水牛源BFF细胞中,EGFP表达水平均显著低于其他物种启动子的细胞转染组(P<0.05),而在鼠源PT67细胞中,水牛启动子的启动效率与其他物种启动子差异不显著(P>0.05).[结论]水牛7SK和U6启动子可高效启动shRNA表达,且具有一定的物种特异性. 相似文献
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AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line. 相似文献
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AIM: To study the change of radiosensitivity of U251 cells after treated with sodium dichloroacetate (DCA) and further to explore the possible mechanism.METHODS: The U251 cells were divided into 4 groups: control group, DCA group, X-ray irradiation without DCA pretreatment (IR) group and X-ray irradiation with DCA pretreatment (DIR) group. MTT assay was applied to determine the cell viability. The intracellular reactive oxygen species (ROS) were detected by DHE fluorescence. The expression level of Bcl-2 was assessed by Western blot. The percentage of apoptosis of cells was determined by flow cytometry. RESULTS: No difference between control group and DCA group in cell viability (P>0.05) was observed. However, the cell viability of both IR group and DIR group was markedly reduced compared with control group (P<0.05). Furthermore, the viability of DIR group was significantly decreased compared with IR group (P<0.05). The percentage of ROS positive cells was obviously increased in DIR group compared with IR group (P<0.05). The expression level of Bcl-2 was sharply decreased in DIR group (P<0.05) and the percentage of apoptosis of cells was significantly elevated (P<0.05) in DIR group compared with IR group.CONCLUSION: The better antitumor effect was obtained by improving the radiosensitivity through pretreating the cells with DCA, and the possible mechanism was down-regulation of the Bcl-2 expression by developing the intracellular ROS. 相似文献
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运用比较分析等方法,对宾夕法尼亚州和上海市学校体育课程设置进行比较分析发现,美国学校体育课程设置内容广泛,重视个体全面发展、注重人文关怀、注重各级学校体育课程衔接;提出上海市学校体育课程设置要提高衔接性、体育课程设置应该重视人文关怀,关注残疾人体育、充分利用体制优势,促进学生养成良好体育锻炼习惯、加强学校、家庭和社区的联动,构建完整体育活动圈等建议。以期为学校体育课程改革提供些许有益的参考,从而进一步促进学生身心健康。 相似文献
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美国油气管道法规和标准体系的管理模式 总被引:3,自引:1,他引:2
介绍了美国油气管道安全管理机构DOT、OPS和TSA及其促进管道安全的措施:建立统一电话报警系统、国家管道地图系统、管道安全促进组织等。阐述了美国油气管道基本法规《管道安全法》《美国联邦规章》《管道安全改进法案》和《管道检验、保护、强制执行和安全法案》的主要内容。研究了与油气管道行业紧密相关、在国际上享有盛誉的美国标准化组织的工作程序和管理模式。研究美国油气管道的管理理念,借鉴其标准规范和经验做法,对于制定我国油气管道行业标准发展战略,建立系统的、与国际接轨的油气管道法规标准体系具有重要意义。 相似文献
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