基于GIS与景观指数的黄骅市景观格局分析

通过对黄骅市栅格影像的景观指数计算,获得10个斑块类型的景观指数和9个景观水平的指数。对黄骅市土地利用类型进行重分类,通过对景观指数的分析,得出黄骅市农业景观、荒漠景观等景观分布格局。结果表明,黄骅市景观整体聚集程度较高、多样性显著、均匀度较高、破碎化程度较高,形成以农业景观为景观基质,荒漠、陆地景观散碎分布,工业、水域景观团块式分布,居住、交通运输景观规律分布的景观格局。     

茶儿茶素影响细胞外多糖合成和变形链球菌附着的研究

通过菌斑量的测定、胞外多糖的提取和测定,对受儿茶素作用后降低酶活性的变形链球菌附着能力进行了体外观察。结果显示,儿茶素浓度在０．１２５％—１．０００％时,细菌附着能力下降,菌斑形成量减少,细菌总蛋白也下降,菌斑中胞外葡聚糖含量降低。由此可见,儿茶素的抗菌斑作用主要表现在抑制胞外葡聚糖产生。     

蓝舌病病毒分离株的定型研究

用国际标准蓝舌病病毒(BTV)型特异性血清和新制备的BTV型特异性血清,按OIE推荐方法进行蚀斑抑制试验,并试用“悬浮法”蚀斑抑制试验,对BTV分离株(L001)作了血清型鉴定,结果表明该分离株为BTV16型。与标准毒株相对照,两者试验结果完全一致;L001株的RNA的PAGE电泳带谱与BTV16型标准株的带谱相同。     

Characterization of a cowpea (Vigna unguiculata) rhizobiophage and its effect on cowpea nodulation and growth

A cowpea rhizobiophage (JRW 3 phage) from Jamaican soil was isolated and characterized. The phage has a polyhedral head and a non-contractile tail; maximum adsorption of the phage to the host occurred after 5 min. A one-step growth experiment revealed that the latent period, rise period and burst size of JRW3 phage were 12 h, 16 h, and 28 plaque-forming units/cell, respectively. The JRW 3 phage was highly sensitive to heat, but survived well between pH 5 and 8. The phage was stable in EDTA, though completely inactivated in sodium citrate. Host range analysis showed that 7 of the 40Rhizobium andBradyrhizobium strains tested were sensitive to phage infection. The phage significantly reduced nodule numbers and shoot dry weight of cowpea plants when inoculated with rhizobia in combination with the phage.     

睾酮及氟他胺对雄兔动脉粥样硬化斑块稳定性与血清炎症因子的调节

目的探讨睾酮和氟他胺对动脉粥样硬化斑块稳定性与血清炎症因子的调节作用。方法主动脉病理切片行HE染色和Masson氏三色染色。血浆睾酮采用Advia Centaur免疫检测系统测定,血清肿瘤坏死因子和白细胞介素6采用放射免疫测定,血清可溶性细胞间粘附分子1和基质金属蛋白酶2应用酶联免疫吸附测定法检测。结果雄兔去睾丸后血浆睾酮水平显著降低,补充十一酸睾酮(每2周6mg/kg)使血浆睾酮恢复到正常水平,而且十一酸睾酮这一作用不受雄激素受体拮抗剂氟他胺的影响。去睾丸雄兔补充十一酸睾酮显著减小动脉粥样斑块的面积和内膜厚度,并使斑块纤维帽增厚、胶原含量增加。然而,同时补充雄激素受体拮抗剂氟他胺使去睾丸雄兔动脉粥样斑块的面积和内膜厚度增加,并使斑块纤维帽厚度减低、胶原含量下降。与假手术高脂喂养雄兔比较,去睾丸雄兔血清肿瘤坏死因子、白细胞介素6、可溶性细胞间粘附分子1和基质金属蛋白酶2显著升高;去睾丸雄兔补充十一酸睾酮后,血清肿瘤坏死因子、白细胞介素6、可溶性细胞间粘附分子1和基质金属蛋白酶2较单纯去睾丸雄兔明显降低;去睾丸雄兔同时补充氟他胺与十一酸睾酮,血清肿瘤坏死因子、白细胞介素6、可溶性细胞间粘附分子1和基质金属蛋白酶2水平再次显著升高。结论睾酮与氟他胺可以调节雄兔动脉粥样硬化斑块进展与斑块的稳定性,并影响其血清炎症因子水平。     

胺苯吡菌酮和叶菌唑混配对5种花卉病菌的联合毒力研究

为寻求防治花卉病害的高效、低毒杀菌剂,采用室内毒力测定方法,以玫瑰锈病菌、文竹叶斑病菌、郁金香灰霉病菌、菊花褐斑病菌和茶花炭疽病菌为测试菌株,研究胺苯吡菌酮和叶菌唑混配对5种花卉病害的联合毒力作用。结果显示:当叶菌唑和胺苯吡菌酮的混配比例为5∶1时,对玫瑰锈病菌的共毒系数为188.16;混配比例为10∶1时,对文竹叶斑病菌的共毒系数为198.07;混配比例为1∶1时,对郁金香灰霉病菌的共毒系数为190.29,对菊花褐斑病菌的共毒系数为192.55;混配比例为3∶1时,对茶花炭疽病菌的共毒系数为200.04。表明胺苯吡菌酮和叶菌唑按适当比例混配对此5种花卉病原菌菌丝和孢子具有明显抑制作用。     

PPARγ gene and protein expression in aortic plaque of different week-old apoE-knock out mice and the relationship with the composition of aortic plaque

AIM: To investigate the relationship of PPARγ gene expression with the composition of aortic plaque in apoE-knock out mice. METHODS: PPARγ gene and protein in aortic area of 20-week-old and 40-week-old apoE-knock out mice were investigated using RT-PCR and immunoblotting. The same aged wild type mice (C57BL/6J) were served as control (n=10). The composition of aortic plaques was analyzed by Movat method and oil red O staining. The expression of antigens such as PPARγ, SM-actin and MOMA-2 in aortic plaque were compared using immunohistochemistry. The relationship of PPARγ with macrophage, smooth muscle cells (SMC), lipid, elastic fiber, collagen and proteoglycan in aortic plaque were analyzed using immunofluorescence. RESULTS: PPARγ gene and protein in aortic wall and plaque of apoE-knock out mice were more significant than that in the same aged C57BL/6J mice (P<0.05). PPARγ expression at 40-week-old apoE-knock out mice was most significant and very low in C57BL/6J mice. More PPARγ expression of gene and protein at 20-week-old C57BL/6J mice than 40-week-old C57BL/6J mice were observed. Compared with 20-week-old apoE^-/- mice, the lipid pool in aortic plaque at 40-week-old apoE^-/- mice were increased remarkably, while elastic fiber, collagen and proteoglycan in plaque were decreased and aortic remodeling was very significant. Even, upregulation of MOMA-2 and downregulation of SM-actin were also detected in latter (P<0.05). In addition to SMC of aortic tunica media, PPARγ also expressed in SMC and macrophages in the aortic plaque of apoE^-/- mice. PPARγ was very enriched in lipid pool of the plaque. CONCLUSION: PPARγ expression level decreases with aging in C57BL/6J mice, while increases with plaque progression in apoE-knock out mice. There is positive correlation between PPARγ expression and lipid composition in plaque. The observed upregulation of PPARγ gene expression in aortic plaque may be a compensatory behavior and protective mechanism in apoE-knock out mice.     

Evaluation of the Logan & Boyce plaque index for the study of dental plaque accumulation in dogs

The objectives of this study were to assess intra-examiner (experienced examiner) and inter-examiner agreements (experienced versus non-experienced examiners) of scores assessed with the Logan & Boyce plaque index and to evaluate whether a modification of this index, where anatomical landmarks are used for horizontal division [mod L&B-AL] and dye references are used for assessing intensity of dye (plaque thickness) [mod L&B-DR], would improve repeatability. The Logan & Boyce index was found to be inaccurate when scoring plaque coverage as it underestimated the total crown surface. The contribution of the gingival part to the total tooth score was minimized by the Logan & Boyce index compared to the mod L&B-AL/DR. Precision of global plaque scorings was significantly improved by the mod L&B-AL/DR. Intra-examiner agreement of plaque thickness and plaque coverage scorings on the gingival part of the tooth was significantly improved by the mod L&B-AL/DR. Studies evaluating plaque accumulation in dogs should therefore use the mod L&B-AL/DR rather than the Logan & Boyce index.     

Expression of galectin-3 in human coronary artery and its correlation with plaque structural stability

AIM To investigate the relationship between the expression level of galectin-3 and the stability of plaque structure in human atherosclerotic plaques. METHODS The coronary specimens from autopsy cases （n=84） were collected. Among them， 22 cases had coronary atherosclerotic lesions without sudden death of coronary heart disease （A1 group）， 20 cases were sudden death of coronary heart disease without secondary lesions （A2 group）， 24 cases were sudden death of coronary heart disease with secondary lesions （A3 group）， and 18 cases without heart disease were used as normal control group （control group）. The intimal thickness， necrotic lesion thickness， fibrous cap thickness and the degree of lumen stenosis were measured by routine HE staining in all coronary arteries. The foam cells in the lesion were marked by CD68 and counted. The expression of galectin-3， CD68 and matrix metalloproteinase-2 （MMP-2） in coronary artery intima was detected by immunohistochemical staining， Western blot and RT-qPCR. The correlation between above factors and the structural stability of atherosclerotic plaques was also analyzed. RESULTS Compared with control group， the intima and necrotic lesions were thickened， the fiber cap was thinned， and the degree of lumen stenosis were increased in A1~3 groups （P<0.05）. The number of foam cells in the atherosclerotic focus was increased （P<0.05）. The protein and mRNA levels of galectin-3， CD68 and MMP-2 in the lesions showed an increasing trend from normal group to A1~3 groups （P<0.05）. The expression of galectin-3， CD68 and MMP-2 in atherosclerotic lesions was positively correlated with intimal thickness and necrotic lesion thickness， and negatively correlated with fibrous cap thickness. CONCLUSION The expression of galectin-3 in human coronary atherosclerotic lesions is increased， which is related to the stability of atherosclerotic plaques.     

利用家蚕生产慈姑蛋白酶抑制剂

将慈姑蛋白酶抑制剂Ｂ（ＡｒｒｏｗｈｅａｄＰｒｏｔｅｉｎａｓｅＩｎｈｉｂｉｔｏｒ，Ｂ，ＡＩＢ）基因插入转移载体ｐＵＢＭ—４中，得到重组转移载体ｐＵＢＭ（ＡＩＢ）；与Ｂｍ—ＮＰＶＤＮＡ共转染家蚕细胞，经空斑纯化，得到不含多角体的重组病毒ＢｍＮＰＶ（ＡＩＢ）；感染家蚕幼虫，利用家蚕高效表达了ＡＩＢ，其表达量为１．０×１０５Ｕ／ｍＬ血淋巴，比活为３．２９×１０３Ｕ／ｍｇ。