Effect of tanshinone ⅡA on liver lipid deposition and ferroptosis-related protein expression in ApoE-/- mice

AIM To observe the effect of tanshinone ⅡA on liver lipid deposition and ferroptosis-related protein expression in ApoE^-/- mice. METHODS Thirty-two ApoE^-/- mice were randomly divided into model group, high-dose (60 mg/kg) tanshinone ⅡA group, low-dose (30 mg/kg) tanshinone ⅡA group and simvastatin group, and C57BL/6J mice (n=8) were used as normal control group. The mice in normal control group were given the basic feeding, while the others were given high-fat diet. The mice in tanshinone ⅡA groups and simvastatin group were given corresponding drugs. The mice in normal control group and model group were intraperitoneally injected with equal volume of saline. Eight weeks later, the serum levels of triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) were tested by automatic biochemistry analyzer. The liver tissues were stained with HE and oil red O. The contents of reactive oxygen species (ROS) and glutathione (GSH) in liver tissues of the mice were measured by commercially available kits. The liver glutathione peroxidase 4 (GPX4) and p53 were detected by immunohistochemical method. The protein and mRNA expression levels of ferroptosis-related factors GPX4, xCT/SLC7A11, p53 and ferritin heavy chain 1 (FTH1) were determined by Wes automatic Western blot quantitative analysis system and RT-qPCR. RESULTS Compared with normal control group, the serum levels of TC, TG and LDL-C in model group were increased significantly (P<0.05 or P<0.01), and HDL-C did not change significantly. The fat vacuoles were clearly visible in liver tissue. The content of ROS in liver tissue was increased significantly,and GSH was decreased significantly (P<0.01). The mRNA and protein expression levels of p53 were increased significantly, and GPX4, xCT/SLC7A11 and FTH1 were decreased significantly (P<0.05 or P<0.01). Compared with model group, tanshinone ⅡA significantly decreased the serum levels of TC, TG and LDL-C (P<0.05 or P<0.01), and HDL-C did not change significantly. High-dose and low-dose tanshinoneⅡA also significantly decreased the degree of steatosis, and the size of lipid droplets. The content of ROS in liver tissues was decreased significantly, and GSH was increased significantly (P<0.01). The mRNA and protein expression levels of GPX4, xCT/SLC7A11 and FTH1 were increased significantly, and p53 were decreased significantly (P<0.05 or P<0.01). CONCLUSION Tanshinone ⅡA reduces liver lipid deposition and lipid peroxidation damage in ApoE^-/- mice, which may be related to the intervention of ferroptosis-related proteins in the liver cells.     

绵羊MHCⅡ类基因遗传多态性研究进展

主要组织相容性复合体(major histocompatibility complex,MHC)是广泛存在于脊椎动物体内的一类高度紧密连锁的基因群,绵羊MHC又称为绵羊淋巴细胞表面抗原(ovine lymphocyte antigen,OLA),位于绵羊20号染色体上,分为Ⅰ类、Ⅱ类和Ⅲ类区域,其中MHCⅡ类基因具有高度的基因多态性.文章综述了绵羊MHCⅡ类基因的分子结构及遗传多态性,重点总结了近十年来国内外不同绵羊品种MHCⅡ类基因遗传多态性的研究进展,并对绵羊MHCⅡ类基因未来的研究重点进行了展望.     

基于VHDL的交通灯控制系统设计

依据EDA自顶向下的设计流程进行交通灯控制系统设计,采用VHDL语言编写各功能模块,生成各模块符号图,把各模块符号图以原理图的形式连在一起得到系统顶层设计。并在Quartus II9.0集成开发环境里进行编译、仿真和综合,最后下载到实验箱进行调试,调试结果表明:交通灯的状态切换,倒计时时间显示均可实现。     

降钙素基因相关肽对猪小肠上皮细胞钠离子依赖型Ⅱb磷转运蛋白表达及磷吸收的影响

本试验旨在研究不同浓度降钙素基因相关肽(CGRP)对猪小肠上皮细胞钠离子依赖型Ⅱb磷转运蛋白(NaPi-Ⅱb)表达和磷吸收的影响.选用第3～8代猪小肠上皮细胞进行试验,按单因素试验设计,将试验细胞分为1个对照组和5个CGRP(1×10-11～1×10-7 mmol/L)试验组,每组设6个重复.用4-甲基偶氮唑盐(MTT)法测定细胞生长速度,采用比色法测定CGRP干预24 h后细胞上清液磷含量,用RT-PCR法检测NaPi-Ⅱb mRNA相对表达量,以及蛋白质印迹(Western blot)法检测NaPi-Ⅱb蛋白表达.结果表明:与对照组相比,不同浓度CGRP对细胞生长没有显著影响(P＞0.05);各试验组均极显著或显著地促进了磷的吸收和NaPi-ⅡbmRNA的相对表达量(P＜0.01或P＜0.05);除1×10-10 mmol/L组外均显著提高了NaPi-Ⅱb蛋白的表达(P＜0.05).研究结果表明,CGRP通过诱导小肠上皮细胞中NaPi-Ⅱb的表达促进细胞磷的吸收,其中以1×10-9、1×10-8 mmol/L浓度的CGRP促进效果较为显著.     

Tanshinone ⅡA induces apoptosis via JNK signaling pathway in human osteosarcoma HOS cells

AIM: To explore the effect of tanshinone ⅡA on human osteosarcoma HOS cells and the underlying mechanism.METHODS: The cell viability and the appropriate dose of tanshinone ⅡA were determined by CCK-8 assay. Colony formation assay and Transwell assay were used to investigate the proliferation and migration abilities of the HOS cells treated with tanshinone ⅡA. The apoptosis of the HOS cells was monitored by Hoechst 33258 staining, transmission electron microscopy and flow cytometry. The protein levels of apoptosis-related molecules and JNK signaling-associated proteins were determined by Western blot. Meanwhile, a JNK inhibitor was added for confirming the relationship between the pathway and apoptosis mentioned above.RESULTS: Tanshinone ⅡA inhibited both HOS cell proliferation and migration in a dose-and time-dependent manner. Exposure of the HOS cells to tanshinone ⅡA resulted in the activation of apoptosis. Tanshinone ⅡA treatment increased the protein levels of cleaved caspase-3, Bax and JNK signaling-associated proteins, and decreased the protein level of Bcl-2, which were reversed by JNK inhibitor SP600125. Moreover, the result of CCK-8 assay revealed that tanshinone ⅡA-induced cell death was alleviated by JNK inhibitor.CONCLUSION: Tanshinone ⅡA induces cell growth inhibition and the activation of apoptosis via JNK signaling pathway in human osteosarcoma HOS cells.     

Study on Developmental Expression of ApoCⅡ Gene mRNA in Liver Tissues of Pigs

The aim of this study was to investigate the developmental patterns of ApoCⅡ gene mRNA in liver in Mashen and Large White pigs, and study the relationship between the expression level of ApoCⅡ and the lipid metabolism in pigs.The mRNA relative expressions of ApoCⅡ gene in liver at seven stages of 1,30,60,90,120,150,and 180-day old in Mashen and Large White pigs were determined by quantitative Real-time PCR.The results showed that the developmental trend of ApoCⅡ mRNA expression in liver between Mashen and Large White pigs was different.The ApoCⅡ mRNA abundance was decreased from birth to 60-day old,then increased at 90-day old,and decreased again after that in Mashen pig.However,the relative expression amount in Large White pig was gradually decreased from birth to 150-day old and increased again at 180-day old.Except for the ApoCⅡ mRNA expression amount at 1-day old,the differences of the expression amount at other stages in Mashen and Large White pigs were significant or extremely significant (P<0.05 or P<0.01).The ApoCⅡ mRNA expression in liver was affected by age and breed,and could play an important role in lipid metabolism in pigs.     

烤烟新品种NC102配套栽培技术研究

为促进优质烤烟新品种NC102在云南省主要烟叶产区的推广种植,2012年在石林县利用L27(313)正交试验设计开展了美引品种NC102的配套栽培技术措施研究。结果表明,在本试验设置的4个处理因素中,施氮量是最主导因素。通过对NC102的经济性状、内在化学成分和感官质量评价等性状的综合分析,最终获得了NC102的最佳栽培技术措施组合。最佳施氮量为每667m2 7.0kg,株距为0.55~0.6m,留叶数为20~22片·株-1,打顶时期为扣心打顶或开花打顶。     

基于Cortex-M4内核的μCOS-Ⅱ移植

针对TI公司新推出的基于Cortex-M4内核的TM4C123G高性能低功耗芯片,详细介绍了嵌入式开源实时操作系统μCOS-Ⅱ在芯片上的移植方法。根据移植的需求,首先介绍了芯片的一些基本功能以及相关的软件开发环境,然后结合芯片的固有特性以及μCOS-Ⅱ移植的需求,使用C语言和汇编语言修改了相关的源文件,并详细阐述了修改的原因。     

Effects of puerarin on apelin-12, angiotensin II,NO and blood pressure in renovascular hypertensive rat

AIM: To investigate the effects of puerarin on blood pressure in renovascular hypertensive rats, and to measure puerarin-induced changes of apelin-12, angiotensin II (Ang II) and nitric oxide(NO), the factors related to development of hypertension. METHODS: Sixty-five male Sprague-Dawley rats were used, of which 8 rats were randomly selected as sham operation group, and the remaining were used to make two-kidney, one-clip model. The rats that met the criterion for Goldblatt hypertensive rat model were randomly allocated into 5 groups: high-, middle- and low-dose puerarin groups, captopril group, and model group. The drugs were administered for 6 weeks. Blood pressure was measured every 2 weeks. Six weeks after treatment, all rats were sacrificed under deep anesthesia. Blood and kidney samples were collected. The level of apelin-12 in serum and kidneys was detected by ELISA. The level of Ang II in plasma and kidneys was measured by radioimmunoassay. NO level in serum was examined by nitrate reductase assay. RESULTS: Puerarin had an antihypertensive effect in a dose-dependent manner. Marked decreases in the level of serum apelin-12 in high- and middle-dose puerarin groups were observed(P<0.01). Puerarin at low dose did not cause obvious change in the content of apelin but still reached significant level (P<0.05). As the dose of puerarin went up, the level of apelin-12 in the kidneys was gradually decreased. Puerarin at high and middle doses obviously reduced the level of AngII in plasma, while purarin at low dose did not produce any significant effects. Puerarin at high and middle doses markedly increased the level of NO in serum, but puerarin at low dose did not induce any significant changes. CONCLUSION: Puerarin has an antihypertensive effect, and its mechanism may be related to inducing the changes of apelin, Ang II and NO, and regulating the balance among those factors.     

活塞中凸型线数控加工的一种逼近方法

针对发动机活塞纵剖面型线的不规则性,提出了采用3次样条插值和双圆弧拟合相结合逼近加工曲线的方法,推导出了适用于计算机编程的3次样条插值计算公式;分析了双圆弧拟合的原理,并对逼近误差进行分析,满足了加工拟合曲线的要求。