Total saponins of Panax ginseng promote hematopoiesis in mice with aplastic anemia

AIM: To observe the effects of total saponins of Panax ginseng (TSPG) on the promotion of hematopoiesis and to explore the underlying mechanism in treating aplastic anemia (AA) in a mouse model.METHODS: For preparation of AA model, BALB/c mice were exposed to sublethal dose of 5.0 Gy γ radiation, followed by transplantation of lymphocytes from DBA/2 donor mice. The experiment was divided into 6 groups, including normal control group, AA model group, TSPG treatment groups with low, medium and high doses, and positive control group with cyclosporine A (CsA). Both TSPG and CsA were administered by gastrogavage. After 15 d treatment, the peripheral hemogram was tested, the cytokine contents in serum were measured, and bone marrow semisolid culture of colony-forming assay was conducted for determining hematopoietic progenitor cells. The protein levels of extracellular signal-regulated kinase 1/2 (ERK1/2) and its phosphorylation status in the bone marrow cells were determined.RESULTS: Curative effect of TSPG in treating AA mice was satisfactory. Peripheral counts of white blood cells and platelet, and concentration of hemoglobin in TSPG groups with medium and high doses were significantly higher than those in model control. TSPG increased the colony numbers of CFU-E, BFU-E, CFU-GM and CFU-MK derived from hematopoietic progenitor cells. Meanwhile, up-regulation of the phosphorylated ERK1/2, decreased contents of Th1 cytokines and increased contents of Th2 cytokines in the serum were observed.CONCLUSION: TSPG possess the dual efficacies on the promotion of hematopoiesis and immunoregulation in AA mice by regulating the expression of Th1/Th2/Th17 cytokines and increasing the phosphorylation of ERK1/2.     

二甲苯对小鼠造血功能的影响

[目的]研究二甲苯及致溶药物盐酸苯肼对小鼠造血功能的影响,为人们日常生活防护和临床诊治提供一定的理论和试验支持。[方法]受试动物昆明小鼠30只,随机分为5组,设置低、中、高浓度二甲苯及盐酸苯肼受试组,以及一个空白对照组。试验期间连续用药进行腹腔注射,第2天起每日检测红细胞数量、白细胞数量、血红蛋白含量以及网织红细胞比例。[结果]血液检测显示用药2 d后小鼠开始出现贫血现象,第5天红细胞数量最高可降低56%左右,血红蛋白含量也随之下降65%左右,网织红细胞比例最高可升高21%左右,当持续用药时,各指标大体维持在同一水平上。[结论]二甲苯对小鼠有致溶血作用,可严重影响小鼠的造血功能。