东北虎电刺激采精研究

对黑龙江东北虎林园的17只东北虎进行了23次电刺激采精,并对取得的精液进行了品质分析。结果表明,不同虎个体对电刺激采精的电压敏感度各异,在平均电压为(4.8±1.3)V时射精。所采集精液量平均为(4.0±1.2)mL,pH值为7.1±0.3,精子密度为(23.4±33.8)×106个/mL,精子活率为(62.9±20.0)%,畸形率为(45.1±15.0)%。从总体上看,12月份和1月份采精效果最好,有精率占全年的57.2%,10月份和11月份的采精效果最差,无精率占全年的44.4%,说明东北虎精液品质受季节影响。     

Effects of anesthetic protocols on electroejaculation variables of Iberian ibex (Capra pyrenaica)

The effects of three intramuscular anesthesia protocols - detomidine 190 μg/kg plus ketamine 2 mg/kg, detomidine 270 μg/kg plus ketamine 1.4 mg/kg, and tiletamine 3.4 mg/kg plus zolazepam 3.4 mg/kg - on penis protrusion and ejaculation variables were compared in nine captive Spanish ibex (Capra pyrenaica) subjected to electroejaculation. Body temperature, heart, and respiratory rates, as well as a number of plasma biochemical variables were also recorded prior to and during anesthesia. The detomidine plus ketamine protocols induced bradycardia and increased respiratory rate. However, the tiletamine/zolazepam protocol did not affect heart and respiratory rates. None of the three protocols caused a substantial change in rectal temperature, yet all protocols caused a significant increase in plasma glucose levels. Differences in anesthetic protocols did not affect sperm quality or quantity. However, choice of anesthetic protocol affected (P < 0.05) the degree of penis protrusion and the electrical pulse sequence required to achieve ejaculation. Results of this study support a recommendation of detomidine 270 μg/kg plus ketamine 1.4 mg/kg for anesthesia of Spanish ibex undergoing electroejaculation.     

非人灵长类动物电刺激采精与精液冷冻保存技术的利用现状

辅助生殖技术在非人灵长类动物上有重要的应用价值,获得高品质精液是有效开展辅助生殖研究工作的基本前提。电刺激采精与精液冷冻保存技术是目前获得高品质非人灵长类动物精液的重要手段。本综述在广泛参阅国内外研究成果的基础上,对当前非人灵长类动物电刺激采精与精液冷冻保存技术的利用现状进行整理和论述,旨在为将来开展非人灵长类动物的辅助生殖技术研究提供参考。