Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.     

稻瘟病菌T-DNA插入的突变表型分析

PCR技术检测28个形态发育和致病性相关T-DNA插入突变体，结果所有突变体均含磷酸转移酶基因序列。对这些突变体展开进一步生物学性状观察，发现15个颜色异常，8个菌落生长缓慢，2个分生孢子形态异常，2个附着胞形态异常，3个不能形成附着胞。致病性测定结果，9个突变体完全不能导致抗瘟（C101）和感瘟（日本晴）水稻苗致病，病害级别均为0级。用标准菌株1528和P131测定突变体有性世代的形成能力，结果发现， Y34-0211、Y34-1469和Y34-0635 3个突变体完全丧失产生有性世代的能力。     

柴油机试车间排气消声系统设计

本文通过理论和实践相结合，因地制宜采用两级插入管扩张室消声结构和隔声、隔热措施对柴油机试车间的排气噪声进行综合治理，取得较好效果，为旧试车间、衣机修理站排气噪声治理提供参考。     

治牛臌气病验方十则

验方一:牛顺气孔插枝治疗臌气病。将牛站立保定好,助手用木棒或竹筒横入口腔内,即见上颌齿版第一条腭褶间的正中处,有一个三角形切齿乳头,其两侧各有一小孔。术者用根火柴棍大小的去皮光滑柔韧而挺直的嫩树枝,如柳树枝、桃树枝等,顺气孔内向上方慢慢插入,直至插不进为止。一般插入长度1.5～2.5厘米,将树枝距孔一二厘米处切断即可,有效率达93.7%。验方二:地牯牛(地拱、蚁狮)、四季葱、蜂蜜各适量。     

进气管路长度对进气噪声的影响

以对某款轿车进气噪声优化为例,介绍了如何通过以改变进气管长度的方式,衰减进气谐振器都难以消除的50-100Hz的进气口低频噪声,并通过GT-POWER模拟计算进气系统的插入损失,得到调整进气管长度对进气噪声影响的规律,最后进行实车验证,车内噪声得到良好改善。     

植物基因捕获系统及其应用研究进展

随着人类和其他一些重要动、植物序列数据的快速积累,我们面临着如何鉴定这些序列数据所代表的生物学功能的巨大挑战。基因捕获技术是一种产生大规模基因突变的便利手段,对于揭示大量基因序列所对应的基因功能具有重要应用价值。本文介绍了植物中的基因捕捉系统及其在植物分子生物学研究中的应用。     

淡紫拟青霉T-DNA插入突变体的表型分析

研究淡紫拟青霉T-DNA插入突变体,鉴定突变体的表型特征,为淡紫拟青霉致病机制分析及致病相关的基因克隆奠定基础。PCR检测筛选的14株T-DNA插入突变体,观察和测定菌落形态,菌落生长速率,产孢量,孢子萌发率和菌丝干质量等生物学性状,并比较突变体致病力。结果表明突变体均含有beta-tubulin基因序列,T-DNA已整合进入野生型菌株20-7的基因组中。通过与野生型菌株对比,发现突变体菌落形态发生了不同程度的改变。菌落生长速率明显增大的菌株占全部菌株的85.71%,只有BN-11菌株的菌落生长速率降低。产孢量显著变大的菌株有BN-11和BN-12,为菌株总数的14.29%。孢子萌发率发生改变的菌株占78.57%。菌丝干质量发生明显增大和减少的菌株都占全部突变体的21.43%。筛选获得了1株致病力增强的突变体BN-11和11株致病力显著降低的突变体。     

KOR调控内切-1,4-β葡萄糖酶在植物生长发育的作用

综述了KOR调控内切-1,4-β葡萄糖酶的结构,以及在植物根伸长发育、纤维生物合成中的作用,并提出了展望。     

AutoCAD实用技巧

本详细介绍了如何把AutoCAD绘制的图形插入到Word档；如何把Excel制作的表格插入列AutoCAD图形中，并转换成AutoCAD图元以及对象选择的几种方式。