不同氮源对红檬实生苗菌根依赖性的影响

将红檬实生苗培养于改良无土基质中，接种泡囊－丛枝菌根Ｇｌｏｍｕｓｃｉｔｒｏｃｏｌｕｍ，以不接种菌根为对照，并分别施用含氮量相等的３种氮肥，即硝酸按（ＮＯ３－Ｎ）、尿素（ＮＨ２－Ｎ）和硫酸铵（ＮＨ４－Ｎ）。接种菌根１６周后，施不同氮肥的红檬实生苗均有效地感染了菌根，并促进了植株的生长。试验证明，红实生苗施硝态氮（ＮＯ３－Ｎ）使植株的菌根依赖性有明显提高。     

The significance of VA-Mycorrhiza to future arable farming in the Netherlands

Present conditions of high inputs and narrow rotations render the importance of vesicular-arbuscular mycorrhiza (VAM) in arable farming in the Netherlands rather low. Changes expected in the near future under the influence of economic pressures and environmental problems may change this situation. This is discussed in the light of some such expected changes in Dutch arable farming.De huidige Nederlandse akkerbouw is zeer intensief. Ze heeft in het algemeen een nauwe rotatie met slechts enkele gewassen (aardappelen, granen en suikerbieten) en een ruime toepassing van kunstmest en bestrijdingsmiddelen. Het is te verwachten dat er onder invloed van toenemende problemen met het milieu, afnemende bodemproduktiviteit en lagere prijzen voor nu geteelde gewassen ten gevolge van overschotten een tendens zal zijn in de richting van een meer geïntegreerde landbouw. In de bestaande akkerbouw werken zowel hoge kunstmestgiften (met name N en P) en bestrijdingsmiddelen negatief op VAM-vorming. Naarmate het gebruik van kunstmest en bestrijdingsmiddelen in de nabije toekomst wellicht zal verminderen, kan worden verwacht dat zowel de aanwezigheid als het belang van VAM in de nederlandse landbouw zal toenemen. Het laatste zal vooral het geval zijn indien de vruchtwisseling wordt verruimd met de teelt van andere gewassen (zoals vlinderbloemigen) met een hogere VAM-afhankelijkheid dan die van het huidige gewasassortiment. Het zou zeer verstandig zijn zich reeds nu te beraden over vormen van bedrijfsvoering die VAM zoveel mogelijk stimuleren en daardoor meer profijt trekken van de door deze symbiose geboden voordelen.     

Effect of biocontrol agents and biofertilizers on root rot,yield, harvest index and nutrient uptake of cassava (Manihot esculanta Crantz)

Cassava is an important subsidiary food and industrial raw material in the tropics. Root rot disease, caused by Phytophthora palmivora, poses a serious threat to cassava cultivation in Tamil Nadu, India. Field experiments (2008–09) were conducted to study the effect of biocontrol agents (Trichoderma spp. and Pseudomonas fluorescens) and biofertilizers (Azospirillum, vesicular–arbuscular mycorrhizal fungi and phosphorus-solubilizing bacteria) on root rot, yield, harvest index and nutrient uptake of cassava at two NPK rates. The design of the experiment was a split plot with two NPK rates, recommended and 50% recommended rate, as the main plot treatments and five biocontrol agents and biofertilizers as subplot treatments. The results clearly indicated that use of a bioinoculants consortium significantly reduced root rot infection/disease incidence over uninoculated controls. Azospirillum significantly improved the yield of cassava at 50% of the recommended rate of NPK. NPK rates had no significant impact on harvest index of cassava and Trichoderma and vesicular–arbuscular mycorrhizal fungi resulted in a higher harvest index even at 50% of the recommended NPK rate. Nitrogen, phosphorus and potassium uptake was significantly improved when treated with biofertilizers and/or a consortium.     

Vesicular-arbuscular mycorrhiza effects on Gliricidia sepium and Senna siamea in a fallowed alley cropping system

Nitrogen fixing and non-N₂ fixing legumes such as Gliricidia speium and Senna siamea have been used in alley cropping systems for soil improvement and source of N for food crops. However their establishments could be limited by P and moisture deficiencies in degraded soils. Vesicular-arbuscular mycorrhizal fungi can help to overcome these deficiencies. We examined the effects of a vesicular-arbuscular mycorrhizal (VAM) fungus, Glomus deserticola, on the performance of sole hedgerow trees of Gliricidia sepium and Senna siamea and their mixtures (interplanted) in a fallowed alley cropping experiment on a degraded Alfisol in southwestern Nigeria. Percentage root infection by VAM fungi was higher in inoculated plants than in uninoculated ones irrespective of whether they were interplanted or non-interplanted. Inoculation with G. deserticola increased dry matter accumulation and nutrient uptake (N. P, Mg and K) but there was no significant interaction between mycorrhizal inoculation and interplanting for growth and nutrient uptake except for the uptake of P, Mg and K in G. sepium. Inoculation with G. deserticola reduced leaf shedding of G. sepium by 50% but did not have the same effect for S. siamea. For both tree species inoculated plants extracted more water from 0–30 cm depth than the uninoculated ones.     

Utilization of Fungi for the Biological Control of Insect Pests and Ganoderma Disease in the Indonesian Oil Palm Industry

Biological control is usually the first choice of control and prevention method for integrated pest management （IPM） strategies and has now been widely implemented by Indonesian oil palm plantations. Entomopathogenic fungus, i.e., Metarhizium anisopliae, Cordyceps militaris and Beauveria bassiana have been demonstrated to control renowned pests of oil palm. Metarhizium has been used to control Oryctes larvae and the mortality has ranged from 91.67% to 100% in laboratory and 7.4% to 88.75% in the field. Metarhizium has been applied in combination with a termite baiting system （TBS） to control termites in the field for preventive and curative action as well. In many oil palm plantations in Indonesia, Cordyceps has been used to reduce the field moth population ofSetothosea asigna. Application of Cordyceps within the oil palm circle was able to infect S. asigna pupae up to 80%. Meanwhile, Beauveria in an effervescent formulation was demonstrated to have better efficacy on Darna trima larvae. A significant finding on the biological control of basal stem rot disease （Ganoderma） was the isolation of Trichoderma sp. and Gliocladium sp.. The efficacy was conducted with promising result and techniques on the application of Triehoderma have been developed, i.e., hole-in-hole system, surgery and a mounding method. However, as the roots developed, Trichoderma was no longer able to protect the palm from Ganoderma. In spite of that, the use of Trichoderma still prolonged the life ofoil palms by up to 2-3 years. Another fungi belonging to vesicular arbuscular mychorrhiza （VAM） has been developed to control Ganoderma. The efficacy in the nursery showed promising results and the Ganoderma incidence remained low compared to the untreated control. Large scale field trials are ongoing. Challenges on the implementation of biological control in oil palm plantations are because of application and availability of biopesticides/natural enemies. Therefore, advances in research on the formulation of biological control agents are still needed.     

Fitting plants to soil through mycorrhizal fungi: Mycorrhiza effects on plant growth and soil organic matter

Summary Vesicular-arbuscular mycorrhizal (VAM) fungi affect diverse aspects of plant form and function. Since mycorrhiza-mediated changes in host-plant responses to root colonization by different VAM fungi vary widely, it is important to assess each endophyte for each specific effect it can elicit from its host as part of the screening process for effectiveness. Three species of VAM fungi and a mixture of species were compared with non-VAM controls for their effects on soil organic matter contents and on nutrition and morphology in two varieties (native and hybrid) of corn (Zea mays L.) and one of sunflower (Helianthus annuus L.) in P-sufficient and N-deficient soil in pot cultures. Differences in soil organic matter due to the fungal applications were highly significant with all host plants. Native corn responded more to VAM colonization than the hybrid did; differences in treatments were significant in leaf area, plant biomass, and root: shoot ratio in the former, but not in the latter. Responses in the sunflower were similar to those in the native corn. Significant VAM treatment-related differences in shoot N and P contents were not reflected in shoot biomass, which was invariant. Correlations between plant or soil parameters and the intensity of VAM colonization were found only in soil organic matter with the native corn, in specific leaf area in the hybrid corn, and in plant biomass in the sunflower. The presence of the different endophytes and not the intensity of colonization apparently elicited different host responses.     

Influence of a vesicular-arbuscular mycorrhizal fungus on the competitive ability of Bradyrhizobium spp. for nodulation of cowpea Vigna unguiculata (L.) Walp in non-sterilized soil

Summary We examined the influence of a vesicular-arbuscular (VAM) fungus (Glomus pallidum Hall) on the competitive ability of introduced and native Bradyrhizobium strains to nodulate cowpeas [Vigna unguiculata (L) Walp]. Our experiments in non-sterilized soil revealed that in the presence of VAM fungus, introduced Bradyrhizobium spp. strains become more competitive than native rhizobia. For example, strain JRC29 occupied 59.2% of the total nodules when inoculated alone, but this figure increased to 71.2% when JRC29 was used in dual inoculations with VAM fungus. A similar pattern of enhanced competitiveness for nodule formation was observed with the two other strains in the presence of the VAM fungus. Our results suggest that the competitiveness of rhizobia can be enhanced by co-inoculating with a selected strain of a VAM fungus.     

Competition among strains of Bradyrhizobium and vesicular-arbuscular mycorrhizae for groundnut (Arachis hypogaea L.) root infection and their effect on plant growth and yield

Summary Strains of Bradyrhizobium influenced root colonization by a species of vesicular-arbuscular mycorrhizae (VAM), and species of VAM influenced root nodulation by strains of Bradyrhizobium in pot experiments. In a field experiment, the effects of VAM on competition amongst inoculated bradyrhizobia were less evident, but inoculation with Bradyrhizobium strains increased root colonization by VAM. Certain VAM/Bradyrhizobium inoculum strain combinations produced higher nodule numbers. Plants grown without Bradyrhizobium and VAM, but supplied with ammonium nitrate (300 g ml^–1) and potassium phosphate (16 g ml^–1), produced higher dry-matter yields than those inoculated with both symbionts in the pot experiment. Inoculation with either symbiont in the field did not result in higher pod and haulm yields at harvest.ICRISAT Journal Article No. 886     

Potential of earthworms,ants, millipedes,and termites for dissemination of vesicular-arbuscular mycorrhizal fungi in soil

We studied the effects of earthworms, termites, ants, and millipedes on the dissemination of vesciulararbuscular mycorrhizal (VAM) propagules. Earthworm (Lumbricus terrestris L.) casts collected from the garden were air-dried, stored, and examined for the presence of VAM fungi by inoculating the cast material onto onion plants grown in sterilized soil. VAM propagules survived for a period of 12 months. The results showed that earth-worms can contribute to the dissemination of VAM propagules. Nests of ants (Camponotus compressus Fabr.) collected from different locations were examined for the presence of VAM propagules. They harboured up to 790 infective propagules g^-1 of nest, showing that ants can be potential vectors in disseminating VAM fungi. The faecal pellets of the millipede (Phyllogonostreptus nigrolabiatus Newport) collected from the rearing tank were tested for VAM colonization. Though VAM propagules were present in the fresh faecal pellets, they lost their viability after 4 days of storage. Termitaria samples of both mound-building and subterranean termites were also examined for VAM propagules but those found were non-viable.     

丛枝菌根对植物生理代谢的影响

参阅国内外大量文献,分析了丛枝菌根在营养生理及抗性生理方面的研究进展。同时,重点就丛枝菌根在促进宿主植物对氮、磷等营养元素的吸收、提高宿主植物抗逆性及抗病性研究进展进行了阐述。