不同保存方法对光皮桦总DNA提取效果的影响

光皮桦是一种顽拗类植物,其鲜叶组织中富含酚类、糖类及其他次生代谢物。这些物质很容易使鲜叶发生褐变,从而严重影响植物基因组DNA的提取质量。本文以新鲜嫩叶为对照,比较了-20℃冷藏、硅胶干燥、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液固定4种鲜叶保存方法对提取光皮桦基因组总DNA效果的影响,寻找最佳的光皮桦嫩叶保存方法。并以提取的核酸得率、纯度、片段分布情况、是否含有PCR反应抑制剂等指标来评价其保存效果。结果表明:-20℃冷藏的样品未能提到DNA;新鲜样品、改进的饱和NaCl-CTAB溶液保存和核分离缓冲液保存的样品均提到纯度较好的DNA,大小在48Kb左右,得率为400!g/g鲜叶左右,并获得条带清晰、多态性好的PCR扩增图谱;用硅胶干燥保存的样品也能提到DNA,但得率低,为51.2!g/g鲜叶,且未获得条带完整、清晰的PCR扩增图谱。     

光皮桦超干种子回湿方法研究

超干处理的光皮桦种子直接吸胀会引起种子活力下降,回湿处理可以减轻吸胀过程所造成的损伤.本试验以超干储藏1年的光皮桦种子,分别经回湿处理中湿度、温度和时间三因素组合的30种预先回湿处理.结果表明：湿度、温度和时间三因素是回湿处理的主要因素;30种处理中有6种处理的发芽率、发芽指数和高于对照,以30℃60%RH回湿4 d显著高于对照,效果最佳.     

Development of stump suckers by betula pubescens at different light intensities

Young trees (4–5 year old) of Betula pubescens Ehrh. growing in a stand in the middle of Sweden (lat. 60°15'N) were cut in early June to stump heights of 0 and 10 cm. The birch stumps were exposed to different relative light intensities (100, 50, 25 and 10%) for 100 days until mid September. Five stumps per treatment were used in a randomized complete block with seven blocks on mesic dwarf‐shrub type with low herbs and three blocks on moist dwarf‐shrub type. The number of sprouting stumps was correlated with relative light intensity and forest type. 2.5 % of the stumps of mesic and 14 % of moist forest type did not produce suckers. On mesic forest type, 7% of the stumps exposed to 10% light did not sprout compared with 1.4, 3.0 and 0% at 25, 50 and 100% light, respectively. At stump height 0 cm fewer sprouting stumps were produced than at 10 cm. The mean height of suckers was significantly lower at 10% light intensity than at other levels. The number of suckers per sprouting stump was not related to light intensity but to forest type. More suckers were produced on mesic than on moist forest type. The dry weight of suckers was significantly lower at 10 and 25% relative light intensity than at 50 and 100%. On moist forest type, the dry weight of suckers was lower than on mesic forest type.     

Dynamics of Betula ermanni population in subalpine vegetation in Changbai Mountain, Northeast China

lntroductionNotonIyaretimberIinesthemostconspicuousvegetationboundaryinhighmountains,buttheyareaIsoanimportantecologicaIboundary(Bruce1996,Thomas1997).NowondersthenthosetimberIineshaveaIwaysattractedresearchersforbothscientificandpracticaIreasons.InChangbaiMountain,forexample,therestorationoftheclimatictimberlineandestabIishmentofanedectiveprotectiveforestarethetWoprincipalobjectivesofhigh-mountainfor-estmanagement.AIsointhisconnection,thepotentiaIresponseoftimberlinestoexpectedclimaticc…     

光皮桦幼林生长节律的观察研究

光皮桦(Betuta luminifera H.Winkl)又称亮皮桦,系桦木科(Betulaceae)桦木属(Betula L)落叶乔木树种.木材呈淡黄或淡褐色,材质细致坚韧、切面光滑、不挠不裂,为优良的军工、航空、建筑、家具和室内装饰用材,也可用于造纸.     

白桦胸径和材积生长率相关关系的研究

根据地位指数的大小将白桦林分划分为好、中、差３个类型，通过没类型样地上的解析木资料分析，证明白桦的胸径和材积生长率存在关良好的相关关系。经过优选，建立了不同类型白桦林分的胸径与材积生长率相关模型，并依此编制出白桦一元材积生长率表，为准确预测白桦积生长率提供了理论上的依据。     

光皮桦组织培养技术研究

对光皮桦带芽茎段组织培养技术的外植体启动培养、丛生芽增殖以及试管苗生根和炼苗移栽等方面开展研究,结果表明,外植体灭菌最优流程:经75%酒精1~1.5 min处理后转入0.1%HgCl2溶液消毒处理7 min;在改良MS+6-BA5.0mg.L-1+NAA 0.01 mg.L-1的培养基上,外植体启动速度最快;在改良MS+6-BA 4.5 mg.L-1+蔗糖40 g.L-1+NAA0.01 mg.L-1的培养基上,外植体增殖系数最高可达6.9倍;在改良WH+IBA 3.0 mg.L-1+NAA 1.5 mg.L-1培养基中,继代苗的生根率最高;试管苗移栽的最佳基质为沙∶田园土=1∶1。     

雾灵山自然保护区白桦林的分布、组成及特征

对雾灵山自然保护区的白桦林进行了研究。该区共计有白桦林1195 hm^2,由34科59属65种维管植物组成。白桦林可以分为乔木层、灌木层、草本植物层和层间植物。白桦林的叶特征是以中小型、草质、非全缘、单叶植物为主。详细论述了其分布、生境、种类组成、外貌特征、结构特征,并对群落的演替动态进行了分析。     

干旱胁迫对白桦实生幼苗叶片蛋白质的影响

The studies on leave protein of Betula platyphylla seedlings by the methods of SDS-PAGE electrophoresis and dimensional gel electrophoresis was concerned. The result indicated that some new proteins such as 29kD pI4.3, 29kD pI4.2, 14kD pI5.3 and 11.5kD pI5.16 protein point were induced by soil drought stress, and some protein disappeared,for instance four protein between 12.4 - 11.5kD pI5.8 - 6.44 in seedlings leaves of B. platyphylla during soil drought stress.     

干旱胁迫对白桦实生苗保护酶活性及脂质过氧化作用的影响

目前 ,活性氧自由基与植物抗逆性关系的研究正在日益深入 (戴金平等 ,1 991 ;王以柔等 ,1 995 ;许长城等 ,1 993;Ｊｏｈｎ ,1 992 ;Ｍａｒｃｅｌａｅｔａｌ.,1 993;阎秀峰等 ,1 999) ,对水分亏缺下活性氧对植物的氧化伤害及植物防御系统的反应也作了大量的研究 (陈立松等 ,1 998;1 999;沈秀瑛等 ,1 995 ;吕庆等 ,1 996;王爱国等 ,1 990 ;许长城等 ,1 993;阎秀峰等 ,1 999;Ｍｉｓｈｒａｅｔａｌ.,1 993)。在正常情况下 ,植物体内活性氧产生与清除处于平衡状态 ,不会导致细胞伤害 ,氧自由基的清除剂可分为酶促和非酶促两大类 (蒋明义等 ,1 996…