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构建感染草莓镶脉病毒(SVBV)森林草莓的酵母cDNA文库,利用酵母双杂交系统,筛选出与SVBV P1蛋白互作的15种寄主因子。生物信息学分析发现,这15种寄主因子参与茉莉酸途径、泛素化、光合作用、抗病抗逆、蛋白修饰、蛋白运输和氧化还原等多种生物过程。另外,这些寄主因子还具有其他分子功能,包括氧化还原酶活性、蛋白二硫化物异构酶活性和金属离子结合活性等。本研究初步探讨了P1与寄主因子的互作机理,为揭示SVBV侵染森林草莓以及SVBV在寄主中扩展的分子机制提供理论依据。 相似文献
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利用自制的应力-应变试验台,对草莓果实的应力-应变特性和蠕变特性进行了测定与研究,建立了草莓果实应力-应变的数学模型,指出了草莓果实的极限屈服点变动范围。同时对草莓在不同贮藏条件下流变特性的变化规律进行了初步的研究,发现草莓的成熟度、贮藏温度及贮藏时间对果实的硬度、屈服应力均有较显著的影响,建立了屈服应力随时间变化的不同阶段的回归方程,可用于预测在相应贮藏条件下的草莓果实的屈服应力。 相似文献
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以微生物拮抗病原菌是防治病害安全高效的可行手段。通过对果实表面微生物进行筛选鉴定,以期得到一株可以有效抑制灰霉病的生物防治菌株。对拮抗菌株H-1进行形态学特征、生理生化特性分析,结合16S rDNA序列分析和gyrB 基因序列分析构建系统发育树;采用平板对峙法结合果实伤口试验考察拮抗菌H-1在体外及梨果表面的抑菌作用。结果表明:经鉴定H-I菌株为贝莱斯芽孢杆菌(Bacillus velezensis);B. velezensis H-1在体外条件下可显著抑制灰葡萄孢霉(Botrytis cinerea)、细极链格孢(Alternaria tenuissima)和串珠镰孢菌(Fusarium verticillioides)的菌丝生长,其中对灰葡萄孢霉菌丝的抑制作用最强;果实伤口试验表明,1×1011 CFU/mL的B. velezensis H-1可完全抑制梨果表面灰霉菌生长。由此可见,B. velezensis H-1可以有效抑制梨果采后灰霉病的发生。 相似文献
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Christiane Stehmann Maarten A. De Waard 《European journal of plant pathology / European Foundation for Plant Pathology》1996,102(2):171-180
Sensitivity of field isolates (121) ofBotrytis cinerea from France (1992), Germany (1979–1992), Israel (1990) and the Netherlands (1970–1989) to the triazoles tebuconazole and triadimenol, the benzimidazole benomyl and the dicarboximide vinclozolin were tested in radial growth experiments. Resistance to benomyl (in 21 to 100% of isolates tested) and vinclozolin (in 25 to 71% of isolates tested) was common in most countries. EC50s (concentrations of fungicides inhibiting radial mycelial growth ofB. cinerea on B5-agar by 50%) for tebuconazole and triadimenol ranged between 0.01–1.64 and 0.4–32.6g ml–1, respectively, and were log-normally distributed. The variation factor (ratio between EC50s of the least and most sensitive isolate tested) amounts 164 and 82 for tebuconazole and triadimenol, respectively. These values are comparable to those for azole fungicides applied in control of other pathogens. Hence, variation in sensitivity to triazoles can probably not explain limited field performance of triazoles towardsB. cinerea. Isolates from south west Germany (1992) were significantly less sensitive to tebuconazole than isolates collected earlier in Germany, Israel and the Netherlands. Such less sensitive populations may contribute to the limited field performance of DMI fungicides towardsB. cinerea. The sensitivity of isolates from south west Germany to tebuconazole was similar to that of DMI-resistant mutants generated in the laboratory. These mutants displayed stable resistance with Q-values (ratio between EC50 of resistant mutant and wild type isolate) between 5 and 20. Sensitivity of field isolates and laboratory mutants to tebuconazole and triadimenol was correlated. 相似文献
6.
灰色灾变理论在宁南山区干旱气候预测中的应用 总被引:15,自引:2,他引:15
在分析宁南山区干旱气候特征及其对农业生产影响的基础上 ,借助灰色灾变理论分别建立了春旱、夏旱、秋旱、春夏连旱、夏秋连旱、全年旱等六种干旱类型的 GM( 1 ,1 )预测模型 ,并对 2 0 0 0~ 2 0 2 0年的干旱气候做出预测。经对 2 0 0 0年和 2 0 0 1年预测结果验证 ,吻合性良好。此外 ,提出了宁南山区抗旱防旱的综合农业措施。研究结果可对广大旱区抗旱减灾 ,促进农业生产发展提供科学指导。 相似文献
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Suppression of wheat-seedling diseases caused by Fusarium culmorum and Microdochium nivale using bacterial seed treatment 总被引:1,自引:0,他引:1
Snow mould, caused by Microdochium nivale , and seedling blight caused by members of the Fusarium complex, are cereal diseases of great economic importance in many temperate zones. In a glasshouse bioassay designed to enhance disease, about 600 plant-associated bacterial isolates obtained by different methods were screened for suppressive effects in wheat against infection caused by Fusarium culmorum . Although most of the isolates tested had a neutral effect on test plants and disease development, a few were synergistic to the pathogen and about one-fifth showed > 80% disease suppression. During five consecutive growing seasons, 164 bacterial isolates were tested in field experiments against both F. culmorum and M. nivale as causal agents of seedling blight. Tests for effects on yield in experiments with spring and winter wheat, performed in different climatic regions of Sweden, showed that disease-suppressive effects were repeatable. The most efficient isolates, three fluorescent pseudomonads and a species of Pantoea , suppressed disease equal to that of the fungicide guazatine, both with respect to crop stand and yield. Seed treatment with Pantoea sp. (isolate MF 626) increased yield by an average of more than 500 kg ha−1 in six field experiments. 相似文献
10.
Chitosan-copper complex compared with chitosan alone enhanced suppression ofBotrytis cinerea rot development on four-true-leaf cucumber seedlings in controlled growth chambers. This paper constitutes the first report
of such enhancement. The optimal concentrations for the most effective suppression ofBotrytis development were 0.2 gl
−1 chitosan and 1.6 mmole copper. After 12 days’ incubation, marked and significantly better disease suppression was obtained
with chitosan-copper complex (75% suppression) than with chitosan alone. The chitosan-copper complex could be a very promising
decay control agent for use in both conventional and organic agriculture.
http://www.phytoparasitica.org posting Dec. 18, 2002. 相似文献