胶体金免疫层析法快速检测烟草环斑病毒

采用柠檬酸三钠还原法制备胶体金颗粒 ,标记烟草环斑病毒的抗体 ,制成免疫层析检测试纸条。检测粗提纯病毒的灵敏度为 1 0 0 0ng/ml,病汁液稀释 1 0 0 0倍后仍可快速检出。对大豆病种子、烟草冻干病叶等不同材料进行检测也有良好的效果 ,1～ 2min即可出现结果。用 9种不同的病毒进行测试 ,未出现非特异性反应。     

我国增加黄金储备的必要性及风险分析

在我国外汇储备巨增,美元贬值背景下,适当增加黄金储备十分必要,但冒然增加黄金储备也会面临风险。具体分析了当前形势下增加黄金储备的必要性及实际增加黄金储备面临的风险,并对增加黄金储备可采取的途径提出了建议。     

黄金梨果锈的成因及防治

通过3a对黄金梨果锈的调查研究，发现黄金梨果锈的症状有锈斑型和锈点型2种。锈斑型多数于套袋前发生，锈点型则主要于套袋后发生，锈果率为83．4％。果锈发生的原因除与其自身特性有关外，还与周围环境和管理水平关系密切。肥水管理、果实套袋和适当早采是预防果锈发生的关键技术，但任何单一的防治技术效果都不好，必须采取综合防治措施。     

Effect of gold nanoparticles on reversing adriamycin resistance of human hepatocellular carcinoma HepG2/ADM cells

AIM: To investigate whether gold nanoparticles (GNPs) reverses adriamycin (ADM), resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM and to explore the potential mechanism. METHODS: The sensitivities of HepG2 cells and HepG2/ADM cells to ADM were tested by MTT assay before and after GNPs pretreatment. The apoptotic rate was examined by flow cytometry. The concentration of ADM in HepG2/ADM or HepG2 cells was determined by ultraviolet-visible spectrophotometer. The content of glutathione (GSH) in HepG2/ADM or HepG2 cells by DTNB method. RESULTS: The half maximal inhibitory concentrations (IC₅₀) of ADM for HepG2/ADM cells were(29.46±1.73) mg/L and (15.18±0.85) mg/L before and after GNPs pretreatment,respectively. The IC₅₀ of ADM for HepG2 cells was (9.16±2.03) mg/L before pretreatment. The apoptotic rate in GNPs+ADM group was higher than that in ADM group (P<0.05). The concentration of ADM in HepG2/ADM group was lower than that in HepG2 group (P<0.01). After GNPs pretreatment, the concentration of ADM in HepG2/ADM cells was higher than that before pretreatment. The content of GSH in HepG2/ADM group was higher than that in HepG2 group (P<0.01). After GNPs pretreatment, the content of GSH in the HepG2/ADM cells was lower than that before pretreatment. CONCLUSION: Gold nanoparticles can reverse ADM resistance of human hepatocellular carcinoma drug-resistant cell line HepG2/ADM, reduce the content of GSH and increase the concentration of ADM in HepG2/ADM cells.     

基于景观视角的防城港万尾金滩滨海护岸探析

防城港东兴市万尾金滩约10km的滨海护岸公路是生态防护、景观步道、旅游集散、防灾避险和渔船停泊等多种功能协调统于一体的滨海防护工程。通过实地调查测绘,基于园林景观的角度从海岸生态防护、水文特征、景观特性三个方面探讨了万尾金滩滨海护岸公路的合理性与不足。指出了滨海护岸工程如何在抵御风浪侵蚀的基础上体现场地的观赏性、便利性、生态性和安全性,以实现滨海护岸工程景观、功能和生态的有机结合。     

白山金矿尾砂堆积坝坝体静力、动力稳定分析研究

本研究选用吉林省白山金矿尾砂堆积坝为目标,对其坝体静力、动力稳定进行分析,以其为细粒尾矿筑坝稳定性研究提供借鉴。试验结果表明,静力稳定的安全系数和动力稳定的安全系数均大于规范规定的安全系数值,所以可以认为在静力、动力情况下,吉林省白山金矿尾砂堆积坝坝体均能保持稳定。     

IBV抗体的银加强金标免疫技术检测

【研究目的】建立可在临床上检测鸡传染性支气管炎抗体的银加强金标免疫技术（SECGA）;【方法】将胶体金标记纯化的兔抗鸡IgG,然后将IBV纯化抗原包被于NCM上（0.4ug/片）,封闭后在膜片上滴加不同稀释度（10×2X）的血清,作用10分钟,洗涤后浸于1：4金标兔抗鸡IgG液中作用60分钟,再银染10分钟观察结果;【结果】SECGA能检出IB阳性血清的抗体＞10×27,而不与ND、EDS76、IBD阳性血清发生有意义的交叉反应（抗体滴度＜10×22）。对鸡IgG的最小检测量为0.88ng。用SECGA、气管环中和试验、ELISA试验检测IB抗体有明显的相关性;【结论】银加强金标免疫技术（SECGA）可用于IB抗体的检测,具有敏感、特异、简单、快速、适于现场诊断等优点,适宜于广大基层单位使用。     

96;金都尔乳油防除加工番茄田杂草试验

田间试验结果表明,96;金都尔乳油在1 296 ～1 728 g a.i./2(90～120ml/667m2)剂量下对加工番茄田禾本科杂草稗草、画眉草、狗尾草有较好的防除效果,对阔叶杂草反枝苋、灰绿藜有一定的防效,对野西瓜苗、苘麻基本无效.在试验剂量下对加工番茄安全.     

Inhibitory effect of AuNPs-Endostar on melanoma lung metastasis in mice

AIM: To observe the influence of gold nanoparticles combined with Endostar (AuNPs-Endostar) on the melanoma lung metastasis of mice and the underlying mechanism. METHODS: C57BL/6 mice (n=24) were selected for constructing the model of spontaneous lung metastasis of melanoma B16-F10 cells. Subsequently, the mice were randomly divided into Endostar group, AuNPs group, AuNPs-Endostar group and model group. After the formation of melanoma, the mice in each group were injected with different drugs through tail vein for 0.1 mL daily. After 9 d, the mice were narcotized for cutting the tumors in situ. After the operation, they were raised for 2 weeks before killed for obtaining the lung tissues to observe the situation of the metastasis. HE staining was utilized for observing the necrosis status of the tumors in situ, while immunostaining was applied for testing the expression of CD31, carbonic anhydrase-IX (CA-IX), vimentin and zonula occludens-1 (ZO-1) in the tumors. RESULTS: Compared with model group, the pulmonary metastasis in the groups with medical treatment was obviously reduced. In AuNPs-Endostar group, the metastasis inhibition rate was the highest, and the tumor necrosis was also decreased obviously, with the significant reduction of CD31, CA-IX and vimentin expression in the tumors and significant increase in ZO-1 expression. CONCLUSION: Compared with using Endostar or AuNPs alone, the combination of AuNPs with Endostar significantly improves the curative effect of inhibiting the pulmonary metastasis of melanoma in the mice. The mechanism may be related to reducing the tumor angiogenesis, norma-lizing the blood vessels and improving tumor hypoxia, thus inhibiting the tumor epithelial-mesenchymal transition, increasing the tight junctions between tumor cells and decreasing the invasiveness.     

四种金鱼的遗传多样性研究

利用随机扩增多态DNA(RAPD)技术对红白龙睛蝶尾、狮头、黑龙睛、红白高头珍珠鳞四个品种金鱼进行基因组DNA的遗传多样性分析。在事先优化的条件下,试验从选用的40个随机引物中筛选出8个有效引物。共检测到72个清晰稳定的位点,大小为300bp～2500bp,其中48个位点具有多态性,各品种的多态位点比例分别为38.98%,40.29%,48.61%,30.56%,Shannon信息指数为0.1704～0.2954。遗传距离显示四个品种金鱼有一定遗传分化,其中狮头与红白高头珍珠鳞遗传距离最大(0.2303),红白龙睛蝶尾与黑龙睛遗传距离最小(0.0784)。利用UPGMA进行聚类分析表明,红白龙睛蝶尾和黑龙睛亲缘关系最密切,其次是狮头,最后为红白高头珍珠鳞。引物OPY17扩增获得的350bp片段为红白高头珍珠鳞的特异分子标记,可用于红白高头珍珠鳞的鉴别。