吴茱萸嫁接方法试验

试验表明:5种嫁接方法苗木成活率均在80％以上,其中以贴枝接和长块削芽接为最高,平均成活率达95％以上,但从快速繁育苗木着眼,以长块削芽接为好。嫁接适期以3月中旬树液流动时为最好。嫁接苗当年在圃地就能开花结果。     

臭檀育苗试验研究

为了探索臭檀育苗技术，对臭檀种子进行干藏、沙藏等不同方法处理以及场圃育苗试验，得出了最佳种子处理方法和育苗管理措施，为臭檀育苗提供了依据。     

吴茱萸苗木繁育技术研究初报

本文阐述了吴茱萸的采种、种子贮藏、播种前预处理、播种、移植等一系列苗木繁育技术。结果认为圃地用塑料薄膜覆盖,早期移植有利于苗木质量的提高。移植时间应不迟于5月上旬,移植密度则以25cmX20cm为宜。     

吴茱萸病虫害防治研究进展

概述了吴茱萸主要病虫害种类,介绍其了预测预报、农业防治、物理防治、化学防治等防治方法,并提出了生物防治技术和基因工程技术在其病虫害研究中的应用前景.     

不同采收时期吴茱萸果实活性成分变化研究

采用HPLC法同时精密测定江西省吴茱萸规范化种植基地的吴茱萸果实在不同采收时期果实内吴茱萸内酯、吴茱萸碱和吴茱萸次碱3种有效成分含量。结果表明:吴茱萸果实中吴茱萸内酯、吴茱萸碱和吴茱萸次碱的含量在不同采收时期存在较大差异,呈现出在采收初期含量快速增加,随后开始逐渐降低的变化规律。确定江西省吴茱萸果实的最佳采收期为8月初。     

Transdermal behaviors comparisons among Evodia rutaecarpa extracts with different purity of evodiamine and rutaecarpine and the effect of topical formulation in vivo

Aim

Evodiamine (EVO) and rutaecapine (RUT), the major active components from Evodia rutaecarpa extract (EE), are recognized as a depended analgesic agent. This study was designed to investigate the effect of purity and chemical enhancers on the transdermal behavior of EVO and RUT, and the pharmacological effect of their topical cream in vivo.

Material and methods

Transdermal delivery across a full thickness pig abdominal skin was detected in vitro by Franz-type diffusion cell, with HPLC for quantification of the permeation of EVO and RUT. The activity of topical cream in vivo was evaluated by a mice pain model induced by formalin and hot plate.

Results

Transdermal characters of EVO and RUT showed a low transdermal rate, long lag time and low cumulative amount. The transdermal rate and cumulative amount could be promoted by lipophilic enhancers, whereas lag time was shortened by hydrophilic surfactant, but these permeation parameters were not markedly influenced by purity of EE (p > 0.05). The effect in vivo was confirmed by analgesic models in topical cream of EE, which produced a significant (p < 0.05) inhibitory effects on pain response in dose-dependent manner.

Conclusion

The purity of EVO and RUT from EE has no significant effect on their permeation through porcine skin, but oleic acid or nerolidol can markedly elevate the transdermal rate of EVO and RUT. High purity of EE is the best choice for topical preparation to increase the drug loading. The effect of EE in vivo is verified by formalin model and hot plate test.     

响应面法优化微波辅助提取吴茱萸多糖工艺

为探索微波辅助提取吴茱萸多糖工艺的可行性,在单因素实验基础上采用三因素三水平响应面分析法,利用软件Box-Behnken实验设计原理,获得二次线性回归方程式(整体模型P0.01)。以多糖提取率为响应值作响应面图,确定微波提取吴茱萸多糖的优化工艺条件修正为:微波功率400 W、提取时间为100S、提取次数2次、料液比为1∶100,吴茱萸多糖实际提取率为21.01%(预测值为21.9%,传统水提仅为12.3%),验证实验表明,所得模型方程能较好地预测实验结果,拟合度较好。     

基于DNA条形码的我国吴茱萸主要栽培种遗传分析和分子鉴别研究

采用ITS2和psbA-trnH DNA条形码组合分析我国吴茱萸主要栽培种的遗传背景,并对其进行分子鉴别。获得的ITS2序列全长223 bp,共获得7个差异位点,其中特异性鉴别位点2个;psbA-trnH序列全长419 bp,获得特异性鉴别位点3个。基于ITS2碱基序列采用邻接法构建系统聚类树,结果表明:吴茱萸、石虎和疏毛吴茱萸的遗传背景差异较为明显,且吴茱萸与石虎的亲缘关系更为接近;嫁接可以使吴茱萸的遗传背景发生较大的变异;并且序列特异性位点可以准确有效地对吴茱萸正品和伪品进行鉴别。     

吴茱萸GAP丰产栽培技术研究

研究了吴茱萸GAP丰产栽培技术,结果表明:以4 m×3 m的种植规格较适宜;抹芽2次可以降低树高65 cm,提高产量118.83%;喷施多效唑500 mg/kg可以有效抑制新梢生长,提高每株产量;以枝条基部留2芽且短截的冬季修剪方法效果较好;采用夏季采后即时更新方法的效果较好;喷施甲基托布津、扑海因对锈病均有较好的防治效果;喷施5%锐劲特500倍液对天牛的防效达100%。     

阳新县吴茱萸母树林营建技术

吴茱萸是兼有生态、经济、社会三大效益的经济林树种,已有1000年的栽培历史.阳新县是湖北省吴茱萸中心产区,在当今林业生态工程建设中,吴茱萸成为阳新县退耕还林的首选树种,为促进阳新县吴茱萸生产,保护吴茱萸资源,省林业局决定在阳新县建立吴茱萸母树林.通过阳新县吴茱萸生产技术的调查,对其母树林建设经验进行了总结分析.