精氨酸和维拉帕米对缓解胎鼠宫内发育迟缓的影响

本研究在日粮中添加精氨酸(L-Arg)和钙阻滞剂维拉帕米,旨在探讨L-Arg通过钙信号对小鼠宫内发育迟缓(IUGR)的影响。将160只雌性小白鼠随机分为4组,对照组饲喂基础日粮,L-Arg组、钙阻滞剂组、L-Arg+维拉帕米组分别在基础日粮中添加0.5%L-Arg、8 mg/kg维拉帕米、0.5%L-Arg+8 mg/kg维拉帕米。在妊娠第10、12、14、16天颈部放血处死母鼠,剖腹取胎鼠、子宫和胎盘等样品,测定相关指标,取14 d母鼠子宫固定于Carnoy’s和多聚甲醛中,分别用作肥大细胞(MC)、HE和免疫组化染色。结果表明:Arg组胎鼠数量、胎鼠体重等IUGR指标显著高于对照组(P0.05),维拉帕米组显著或极显著低于Arg组(P0.05或P0.01);L-Arg组子宫MC数显著低于对照组(P0.05),极显著低于维拉帕米组(P0.01);Arg组子宫巨噬细胞数较对照组显著增多(P0.05),维拉帕米组较对照组显著减少(P0.05),Arg组与维拉帕米组差异极显著(P0.01);L-Arg组子宫微血管(MVD)数较对照组显著增多(P0.05),较维拉帕米组显著减少(P0.05)。综上,L-Arg通过调控MC释放介质来提高母鼠的繁殖能力,维拉帕米能阻止Arg该作用,表明L-Arg通过钙信号发挥生物学作用,是其缓解IUGR的机理之一。     

Effect of external counterpulsation on asymmetry diethylarginine and endothelin levels in blood

AIM:To observe changes of serum asymmetry dimethylarginine (ADMA) and endothelin-1 (ET-1) levels,which reflect blood vessel endothelial function,after therapy of enhanced external counterpulsation(EECP) in patients who had taken percutaneous transluminal coronary angioplasty and stent.METHODS:Fifty one coronary heart disease patients (all of them had taken percutaneous tranluminal coronary angioplasty and stent) were distributed into two groups by matching them with ratio of 1∶2,17 patients in EECP group and 34 patients in control group.Both of two groups were given conventional medicine,in addition,EECP group was undertaken three courses of treatment of EECP.ADMA was detected by HPLC-fluorescence method,and ET-1 was detected by radio-immunity method.RESULTS:In EECP group ，compared with prior treatment,ADMA and ET-1 levels was obviously reduced (P<0.05).In control group,compared with prior treatment,ADMA had no significant difference (P>0.05) and ET-1 was higher (P<0.01).Compared between two groups,extent of decrease in ADMA and ET-1 levels in EECP group were more obvious than control group (P<0.01).Improvement of angina and decrease of frequency of angina was more obvious in EECP group than control group.Besides,decrease of ADMA level was positive correlation with them (r=0.85，0.70,respectively P<0.01).CONCLUSION:EECP reduces serum ADMA and ET-1 levels by increasing shear stress to vessel endothelium in coronary heart disease patients who had taken percutaneous tranluminal coronary angioplasty.It hints that EECP can improve endothelial function and provides experimental evidence for the combined treatment of coronary heart disease.     

催产素和加压素在犬泡状绦虫的免疫组化定位和分布

应用免疫组化ＡＢＣ法研究了催产素和加压素在犬泡状绦虫的定位和分布。在犬泡状绦虫中枢神经系统的脑神经节、２条主要纵神经索和环神经内观察到大量阳性纤维和少量阳性细胞。周围神经系统可见阳性神经纤维在皮层和实质形成神经网。从纵神经索和环神经发出的阳性纤维向生殖器官和皮层的肌肉分布。顶突的小钩之间的肌肉存在阳性反应物，小钩周围有较明显的阳性反应环。生殖器官呈现着色深浅不同的阳性反应，以卵囊—梅氏腺复合体最为明显。结果表明，催产素和加压素的免疫反应物具有相同的分布，它们可能参与虫体的神经内分泌调节。     

鱼类精氨酸营养生理研究进展

精氨酸为鱼类的必需氨基酸之一，其在鱼体内参与蛋白质、多胺的合成，促进生长；精氨酸具有促内分泌作用；精氨酸可在体内生成一氧化氮，一氧化氮参与鱼类心血管活动、免疫功能的调节。某些鱼类可能具有自身精氨酸的合成能力。鱼类赖氨酸一精氨酸拮抗存在种问差异。本研究从精氨酸的合成、生理功能、与赖氨酸的关系以及需求量等方面对鱼类精氨酸的营养生理研究进行了综述。[中国水产科学，2006，13（4）：679-685]     

精氨酸和B9配合使用对月季切花的保鲜效应

初步研究了不同浓度的精氨酸和B9及其不同配方的使用对月季切花保鲜效果的综合影响。结果表明,精氨酸在改善月季切花的外部形态、推迟最大花径达到的时间、调节月季切花花枝对水分的吸收、延缓花青素的降解、延长瓶插寿命等方面均有较明显的作用。B9可提高切花的最大花径,延长瓶插寿命,改善水分状况。精氨酸和B9配合使用可明显改善月季切花的外部形态,增加切花的最大花径,延长达到最大花径的时间和瓶插寿命。同时还能保持月季切花花枝鲜重、维护切花的水分平衡,延缓切花花青素降解速度。综合评定0.1 mmol/L精氨酸与300 mg/L B9组合处理的保鲜效果最好。     

Changes of neuropeptides and electrolytes in patients with acute traumatic brain injury

AIM: To investigate the levels of neuropeptides and electrolytes in the patients with acute traumatic brain injury.METHODS: Seventy-eight patients with acute brain injury were divided into mild, moderate and severe groups according to their GCS scores. The serum levels of arginine vasopressin (AVP) and angiotensin II (Ang II) were measured on day 1, 3 and 7 after injury. The serum levels of electrolytes were also measured on day 1. Forty-one subjects who received healthy check-up served as normalcontrols. RESULTS: Compared with normal control group, the serum levels of AVP and Ang II significantly increased in the patients with traumatic brain injury (P<0.01), depending on the severity of brain injury. Both neuropeptides reached the peak on day 3 after injury. The concentrations of serum potassium and calcium decreased in the patients with acute brain injury(P<0.01),also showing a severity-dependent tendency. No significant change of serum sodium in the patients with brain injury was observed. CONCLUSION: The serum levels of arginine vasopressin and angiotensin II canalso be used as the severity indicators of traumatic brain injuries. Decrease in serum potassium and calcium can also be used to evaluate the severity in patients with acute traumatic brain injury.     

鸡下丘脑加压素免疫反应神经元的分布——PAP法研究

鸡加压素(VP)免疫反应神经元分布于视上核、室旁核的中部和后部、视上交叉核、视前大细胞核、室周弓状核、下丘脑外侧核和室周核,正中隆起、视上背侧交叉和隔内侧区存在大量的VP阳性纤维末梢.在第三脑室室管膜和脑基底神经胶质板上也存在VP阳性神经元.同时发现室旁核、室周核、室周弓状核及视前大细胞核的VP阳性神经元有突起伸入到第三脑室室管膜或突出于脑室腔,视上核的VP阳性神经元也有突起投射至脑基底神经胶质板上或伸至蛛网膜下腔.据此认为,VP的释放是多途径的.     

Effect of L-arginine on intimal proliferation and expression of related cell cycle regulatory factors after vascular injury in rats

AIM:To investigate the effect of L-arginine (L-Arg) on intimal proliferation and expression of related cell cycle regulatory factors after vascular injury in rats. METHODS:Rats were divided into three groups :sham operation group, balloon injury group(this group included balloon 48 h,7 d and 14 d subgroup) and balloon+L-Arg group. Neointima area were calculated morphologiocally. The expression of cyclin dependent kinase-2(CDK2),cyclin E and proliferation cell nuclear antigen (PCNA) were measured by means of immunohistochemical technique and computer image analyzer. RESULTS:After vascular balloon injury, the level of plasma NO decreased, CDK2、cyclin E and PCNA expressed in the media at 48 h and in the neointima at 7 d and 14 d but with low and undetected expression in the media, the expression of CDK2, cyclin E and PCNA increased with the intima thickening. Compared with balloon 14 d group, the plasma NO level increased (P＜0.01), the neointima area reduced by 59.1%(P＜0.01) and the positive expression indexes of CDK2, cyclin E and PCNA decreased by 36.1%, 46.3% and 76.2% respectively in balloon+L-Arg group (P all＜0.01). CONCLUSION:L-Arg can effectively repress intima proliferation after vascular injury, which may be associated with its inhibiting the proliferation of vascular smooth muscle cell through downregulating the excessive expression of CDK2, cyclin E and PCNA.