Characterization of bovine ileal epithelial cell line for lectin binding,susceptibility to enteric pathogens,and TLR mediated immune responses

In this study, primary and immortalized bovine intestinal epithelial cells (BIECs) were characterized for the expression of surface carbohydrate moieties. Primary BIEC-c4 cells showed staining greater than 90 % for 16 lectins but less than 50 % staining for four lectins. Immortalized BIECs showed significantly different lectin binding profile for few lectins compared to BIEC-c4 cells. BIEC-c4 cells were studied for infectivity to E. coli, Salmonella enterica, bovine rotavirus, bovine coronavirus, and bovine viral diarrhea virus. Bovine strain E. coli B41 adhered to BIEC-c4 cells and Salmonella strains S. Dublin and S. Mbandaka showed strong cell invasion. BIEC-c4 cells were susceptible to bovine rotavirus. LPS stimulation upregulated IL-10, IL-8, and IL-6 expression and Poly I:C upregulated TLR 8 and TLR 9 expression. This study provides important knowledge on the glycoconjugate expression profile of primary and immortalized BIECs and infectivity and immune responses of primary BIECs to bacterial and viral pathogens or ligands.     

Factors driving natural regeneration beneath a planted urban forest

Cities around the world are investing in urban forest plantings as a form of green infrastructure. The aim is that these plantations will develop into naturally-regenerating native forest stands. However, woody plant recruitment is often cited as the most limiting factor to creating self-sustaining urban forests. As such, there is interest in site treatments that promote recruitment of native woody species and simultaneously suppress woody non-native recruitment. We tested how three, common site treatments—compost, nurse shrubs, and tree species composition (six-species vs. two-species)—affected woody plant recruitment in 54 experimental plots beneath a large-scale tree planting within a high-traffic urban park. We identified naturally regenerating seedling and sapling species and measured their abundance six-years after the site was planted. This enabled us to examine initial recruitment dynamics (i.e. seedlings) and gain a better understanding of seedling success as they transition to the midstory (i.e. saplings). Seedling and sapling recruitment (native and total) was greater in areas with higher canopy cover. The combination of the nurse shrub treatment with compost and species composition (six-species) treatments increased seedling recruitment by 47% and 156%, respectively; however, the nurse shrub treatment by itself decreased seedling recruitment by 5% and native seedling recruitment by 35%. The compost treatment alone had no effect on the total number of recruits but resulted in 76% more non-native seedlings. The sizes of these treatment effects were strongly dependent on whether the forest plantings were in open areas, versus areas with existing tree canopy, the latter condition facilitating recruitment. Our findings therefore suggest that combinations of site treatments, paired with broad canopy tree species, may be most effective for promoting regeneration of native species resulting in more self-sustaining urban forests.     

裂脖跳小蜂属(膜翅目:跳小蜂科)1新种及1新记录种记述

记述了裂脖跳小蜂属1新种--韩氏裂脖跳小蜂(Rhopus hanni Zu&Li, sp. nov.),1中国新记录种--硫磺裂脖跳小蜂(Rhopus sulphureus(Westwood,1837))。对新种及新记录种的形态特征和分布进行了详细的描述,并附有成虫特征照片。     

CRISPR/cas系统及其在家禽上的应用研究进展

CRISPR/cas是一种获得性免疫防御系统,广泛存在于细菌和古细菌中。Strep tococcus pyogenes cas9(Spcas9)作为目前研究最多、最清楚的效应蛋白,因其酶活性高和靶点广而被应用于生命科学各个领域,但Spcas9分子量大、脱靶率高等缺点在一定程度上限制了其应用。随着CRISPR系统技术被深入开发,一些新效应蛋白被发现,本文就CRISPR/cas系统分类、原理、新效应蛋白发现及cas9(S.Pyogenes)多种变构体技术开发在及家禽上的应用进行综述,为相关领域的研究提供参考。     

环境温度和饲粮营养浓度对生长期母兔代谢能利用率的影响

选用6只9周龄新西兰母兔,评定环境温度和饲粮营养浓度对生长期母兔代谢能利用率的影响。试验在密闭式的小动物呼吸测热柜中进行。分别对处于环境温度为20±2℃和30±2℃,采食两种不同营养浓度的试兔进行测试。饲粮营养水平实测值,分别为12.22kJ ME/g和13.49kJ ME/g;粗蛋白质(CP)分别为17.3％和21.6％;中性洗涤纤维(NDF)分别为23.7％和16.5％。结果表明:环境温度影响试兔的能量平衡,它与采食量间存在着显著的相关。20±2℃时,相关系数r=0.798;30±2℃时,相关系数r=0.875。在本试验设计范围内,饲粮营养浓度并不影响代谢能食入量(ME_I),热增耗(HP)和能量平衡(EB)。当环境温度为20±2℃和30±2℃时,表观代谢能转化率(NE/ME)分别为51％和71％;每天每兔每千克代谢体重代谢能维持需要分别为396 kJ和361 kJ。试验结果认为:环境温度影响营养物质消化率(P相似文献   

单克隆抗体ELISA检测犊牛粪便中冠状病毒抗原

用两株单克隆抗体混合后包被ＥＬＬＩＳＡ微孔板，加牛冠状病毒抗原，加经白陶土处理过的兔抗牛冠状病毒免血清，再加上辣根氧化物酶标记的羊抗兔ＩｇＧ抗体，加底物质显色间接ＥＬＩＳＡ检测牛冠状病毒抗原获得成功。用此方法检测细胞培养的牛冠状病毒上清液，其敏感度高出血凝试验（ＨＡ）８倍，从武汉市附近３个奶牛场采集犊牛腹泻粪便１０５份，用单抗ＥＬＩＳＡ检测牛冠状病毒抗原，共检出阳性３６份，并与血凝及血凝抑制试验，     

春小麦新品种互麦14号栽培技术

介绍了我省育成的春小麦新品种—互麦14号的丰产栽培技术。     

新型冬暖式日光温室的研究

针对普通型日光温室存在的主要缺陷 ,对普通型日光温室进行了改良研究 ,设计出一种能够克服普通型日光温室缺点的新型冬暖式温室 ,经过试验、示范 ,达到了降低造价、增加有效使用面积、增强保温效果、提高经济效益的目的     

我国南方部分地区实蝇记述及2种中国新记录

1980～1988年,曾用地中海实蝇、瓜实蝇和桔小实蝇(Trimedlure,Cuelure 和 Methy eugonol)性引诱剂,和野外采果相结合的办法,进行了实蝇的调查,所采集的标本,已鉴定出16种,其名称是;桔小实蝇、芒果实蝇、番石榴实蝇、辣椒实蝇、颜带实蝇、鳄梨实蝇、瓜实蝇、南瓜实蝇、颜黑纹实蝇、具条实蝇、拟具条实蝇、腹眼实蝇、二态实蝇、蜜柑大实蝇、桔大实蝇和黑肩光顶实蝇.内鳄梨实蝇和腹眼实蝇2种为中国新记录.在这次调查中,未发现地中海实蝇(Ceratitiscapitata Wied.)。