首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   93篇
  免费   12篇
  国内免费   9篇
林业   1篇
农学   19篇
  4篇
综合类   21篇
农作物   8篇
水产渔业   58篇
畜牧兽医   1篇
植物保护   2篇
  2022年   1篇
  2021年   3篇
  2020年   3篇
  2019年   5篇
  2017年   7篇
  2016年   2篇
  2013年   3篇
  2012年   2篇
  2011年   5篇
  2010年   3篇
  2009年   5篇
  2008年   9篇
  2007年   15篇
  2006年   8篇
  2005年   9篇
  2004年   6篇
  2003年   3篇
  2002年   4篇
  2001年   5篇
  2000年   5篇
  1999年   2篇
  1998年   1篇
  1997年   1篇
  1996年   1篇
  1995年   1篇
  1994年   2篇
  1993年   1篇
  1992年   1篇
  1988年   1篇
排序方式: 共有114条查询结果,搜索用时 31 毫秒
1.
激活标签法是近年来新发展起来的一种研究基因功能有价值的补充方法。该文综述了激活标签法的研究进展及其在番茄功能基因组研究中的应用。  相似文献   
2.
The genetic contribution of 51 broodstock, comprising 29 females and 22 males, reared at Hiroshima City Marine Products Promotion Center for the production of stocked black sea bream was monitored during two consecutive years using seven microsatellite DNA loci. The high discrimination ability of these markers was reflected in the polymorphic identification content (PIC=0.831), the exclusion probability (Q≈1), and the low probability of identity index (I=3.635−10). The total number of breeders contributing to the mating process was estimated at 32 (62.7%) in 2000 and 30 (58.8%) in 2001. On pedigree reconstruction, 69.3% of the offspring were successfully assigned to a single broodstock pair. Loss of alleles accounted for 16.9% during seed production; nevertheless, 90.9% of males and 69.0% of females participated in the mating process. Based on microsatellite genetic tagging, 58.9% of the fish sampled during the two months after release were identified as hatchery stock, presenting no significant differences from wild conspecifics in either fork length or body weight.  相似文献   
3.
Abstract –  Human-induced habitat alteration is one of the main causes of the decline of freshwater fish populations. The watershed of the River Bidasoa (Spain) is an example of heavily fragmented habitat. The local brown trout ( Salmo trutta L.) population is disturbed, with evidence of poor recruitment as well as low adult densities in the main stem. Forty male and female adult migratory trout were tagged with external or internal radio transmitters and released. Fixed stations with data loggers and mobile antennae were used with daily surveys to track fish movements during the migration and spawning period (3 months). Migration distances did not exceed 10 km, and half of the fish never entered a tributary in the study area. Fragmentation because of weirs on the main stem apparently prevented fish from reaching their spawning destination. Fish that entered the tributaries were first confronted with an accessibility problem because of low discharge. However, each fish chose one tributary, without making attempts to run up in other tributaries. Once in the tributary, fish were restrained in their upstream movements by dams. The study area appeared to be isolated from the vast upper part of the watershed. Within the study area, upper parts of tributaries also seemed strongly disconnected from the main stem. This study illustrates the negative impact of river fragmentation on S. trutta migration pattern. Population sustainability can be directly affected through the low availability of spawning grounds for migratory fish. Long-term effects of fragmentation may cause reproductive isolation within watersheds, which in the case of trout also means isolated phenotypic population units.  相似文献   
4.
The Alternaria stem canker disease of tomato is caused by the fungal pathogen Alternaria alternata f. sp. lycopersici and its host-selective AAL-toxins. Resistance to the pathogen and insensitivity to the toxins are conferred by the Asc locus on chromosome 3L. Sensitivity to AAL-toxins is a relative character; the toxins inhibit development of all tested tomato tissues but susceptible cultivars are much more sensitive than resistant cultivars. In addition to tomato, some other plant and animal species are sensitive to the toxins as well. The likely mode of action of AAL-toxins is interference with sphingolipid biosynthesis by specific inhibition of ceramide synthase activity. To molecularly isolate Asc, transposon tagging and positional cloning strategies are applied. As a first step, transposon insertions and restriction fragment length polymorphism (RFLP) markers are identified in proximity of the Asc locus. Subsequently, the transposons are used to inactivate Asc by insertion mutagenesis, and the RFLP markers are used to identify yeast artificial chromosomes (YACs) with tomato DNA inserts. Once an Asc-insertion mutant and/or a YAC encompassing Asc has been obtained, physical isolation and characterisation of Asc will be conceivable. Elucidation of the molecular role of Asc will illuminate the specificity of host recognition by Alternaria alternata f. sp. lycopersici.Abbreviations AAL-toxin Alternaria alternata lycopersici-toxin - A. a. lycopersici Alternaria alternata f. sp. lycopersici - Asc Alternaria stem canker - HST host-selective toxin  相似文献   
5.
Summary A RAPD marker, linked to the Tm-2 agene engendering TMV resistance in tomatoes, was identified. The validity of the RAPD marker was corroborated by screening several tomato varieties, and correctly identifying those which carried Tm-2 a, as well as by F2 segregation analysis. All tested resistant varieties descending from a common Lycopersicon peruvianum/esculentum ancestor, LA1791, exhibited this marker.  相似文献   
6.
拟南芥抗病基因克隆的策略及利用   总被引:2,自引:0,他引:2  
生物技术的快速发展为植物抗病基因的克隆奠定了坚实的基础。克隆抗病基因不但有利于深入研究植物与病原物的分子互作机理,而且为植物重要病害的防治提供了有效措施。迄今为止,在拟南芥中已经克隆了十几个抗病基因。对拟南芥抗病基因的种类和特性、克隆策略以及克隆抗病基因的应用前景进行了综述。详细介绍了定位克隆技术和转座子标签技术的原理及其在拟南芥抗病基因克隆中的应用。  相似文献   
7.
农杆菌介导的T-DNA标签法是近年发展的一种有效的分子生物学技术.综述了T-DNA标签法的特点及其在水稻功能基因组研究中应用的进展.  相似文献   
8.
Satellite telemetry is used routinely to collect and transmit movement, behaviour and environmental data from free‐ranging animals. Satellite tags (hereafter SATs) are essential for collecting in situ data from animals where manual retrieval of a data logger is impossible. The collected data is fundamental for scientific and many management applications. However, the amount of data a SAT can transmit is limited by its battery life, transmission opportunities, bandwidth and satellite coverage. When designing a SAT study, these factors should be taken into account to ensure that limited transmissions are utilized as effectively as possible. In this paper we present a theoretical discussion of the factors that influence the data transmission and present tools for designing effective SAT studies. We calculate the optimal sampling period for the tag given its expected lifetime and regional variation in satellite coverage. Taking these factors into account allows prediction of the expected number of tags required to meet targets for a deployment. These methods are applied to a case study of a highly migratory pelagic fish tagged with popup archival satellite tags (PSATs). Our findings will be useful for SAT study design in general and demonstrate an approach which can reduce the ad hoc nature of SAT deployments.  相似文献   
9.
采用切鳍法和挂牌法对体长为11~17cm、体重50~70g的草鱼(Ctenopharyngodon idellus)进行标志研究,用铜丝从背鳍第一与第二支鳍骨间缝隙穿过,将椭圆型塑料标牌(长8mm×宽5mm×厚1mm)挂在背鳍基前,标志牌及铜丝的平均总重量为0.04g,不影响草鱼正常游泳和摄食等活动;暂养10d的标志牌保持率达到82.4%,土塘饲养70d的成活率为91.5%,标记保持率为79.9%。切鳍法标记草鱼暂养10d后,标志保持率100%,土塘饲养70d后,成活率为83.6%,80.7%的草鱼被剪鳍条已完全或接近完全再生,标记保持率只有19.3%。挂牌标记比切鳍法标记的成活率高7.9%。需要2周时间进行食性转化训练,将饲料食性转回天然食性即草食性。研究结果显示,切鳍法因切除的腹鳍可以完全再生,只适合短期标志(约30d);挂牌法具有较高的标志保持率、标牌保留时间较长和标牌醒目等特点,可用于较长时间的试验研究。2种标志方法均可应用于草鱼增殖放流的效果评估。  相似文献   
10.
Abstract Telemetry was used to examine spawning migration of sea trout, Salmo trutta L. (n = 126), in two rivers in northern Sweden. The spawning areas defined by radio‐tagged fish differed between the river systems. In the River Vindelälven, sea trout spawned in the main stem and 80% of tagged individuals returned to areas where hatchery‐reared juveniles had been previously stocked. In the River Piteälven, 74% of tagged sea trout ascended tributaries for spawning. Tagged fish were categorised into three groups of migratory pattern. cart (classification and regression tree) analysis indicated that distance from tagging location to spawning site (Sdist) explained the migratory patterns. Large Sdist separated fish with stepwise upstream migration from those with up‐ and downstream migrations and one‐directional direct migration. Fish tagged early in the season migrated the longest distance to spawning areas. Stocking locations and sex explained the large search behaviour up‐ and downstream in the rivers. The findings are important for the sustainable management of sea trout in the Gulf of Bothnia.  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号