Long-term fertilization effects on active ammonia oxidizers in an acidic upland soil in China

The effects of long-term fertilization of acidic soils on ammonia-oxidizing archaea (AOA) and bacteria (AOB) communities and its ecological implications remain poorly understood. We chose an acidic upland soil site under long-term (27-year) fertilization to investigate ammonia oxidizer communities under four different regimes: mineral N fertilizer (N), mineral NPK fertilizer (NPK), organic manure (OM) and an unfertilized control (CK). Soil net nitrification rates were significantly higher in OM soils than in CK, N or NPK soils. Quantitative analysis of the distribution of amoA genes by DNA-based stable isotope probing revealed that AOA dominate in CK, N and NPK soils, while AOB dominate in OM soils. Denaturing gradient gel electrophoresis and clone library analyses of amoA genes revealed that Group 1.1a-associated AOA (also referred to as Nitrosotalea) were the most dominant active AOA population (>92%), while Nitrosospira Cluster 3 and Cluster 9 were predominant among active AOB communities. The functional diversity of active ammonia oxidizers in acidic soils is affected by long-term fertilization practices, and the responses of active ammonia oxidizers to mineral fertilizer and organic manure are clearly different. Our results provide strong evidence that AOA are more highly adapted to growth at low pH and low substrate availability than AOB, and they suggest that the niche differentiation and metabolic diversity of ammonia oxidizers in acidic soils are more complex than previously thought.     

Contribution of Different Organs to Grain Filling in Durum Wheat under Mediterranean Conditions I. Contribution of Post‐Anthesis Photosynthesis and Remobilization

Under Mediterranean conditions, drought affects cereals production principally through a limitation of grain filling. In this study, the respective role of post‐anthesis photosynthesis and carbon remobilization and the contribution of flag leaf, stem, chaff and awns to grain filling were evaluated under Mediterranean conditions in durum wheat (Triticum turgidum var. durum) cultivars. For the purpose, we examined the effects of shading and excision of different parts of the plant and compared carbon isotope discrimination (Δ) in dry matter of flag leaf, stem, chaff, awns and grain at maturity and in sap of stem, flag leaf, chaff and awns, this last measurement providing information on photosynthesis during a short period preceding sampling. Source–sink manipulations and isotopic imprints of different organs on final isotope composition of the grain confirmed the high contribution of both carbons assimilated by ears and remobilized from stems to grain filling, and the relatively low contribution of leaves to grain filling. Grain Δ was highly and significantly associated with grain yield across treatments, suggesting the utilization of this trait as an indicator of source–sink manipulations effects on grain yield. Chaff and awns Δ were better correlated with grain Δ than stem and leaf Δ, indicating that chaff were more involved in grain filling than other organs. Moreover, in chaff, sap Δ was highly significantly correlated with dry matter Δ. These results suggest the use of Δ for a rapid and non‐destructive estimation of the variation in the contribution of different organs to grain filling.     

土地利用变化对土壤水补给来源影响研究

为探究不同植被类型深剖面土壤水补给来源,以黄土区长武塬为研究区,采集两种土地利用方式(农地、18 a苹果园、26 a苹果园)下20 m深土壤剖面,测定土壤水及其稳定性和放射性同位素含量,基于同位素方法对不同土地利用类型下不同深度土壤水来源进行溯源分析。研究表明：(1)苹果园土壤水含量明显低于农地,说明果园耗水强度更大,对深层土壤水影响更为显著。(2)农地、18 a和26 a苹果园下降水偏移量分别为-22‰、-20‰和-6‰,说明降水补给土壤水后受到蒸发作用影响,且农地土壤水比果园受到的蒸发效应更强。(3)该区土壤水补给主要以活塞流为主;对于补给不同深度土壤的可能水源,6 m以下补给水的同位素组成(δ²H=-83.8‰,δ¹⁸O=-12.1‰)较0～6 m (δ²H=-68.8‰,δ¹⁸O=-10.1‰)更为贫化,且6 m以下补给水的氢氧稳定同位素组成与日降水量≥50 mm·d^-1的降水更相似。与农地(δ²H=-70.6‰,δ¹⁸O=-...     

稻田水体碳氮稳定同位素组成与时空变化研究

利用稳定同位素技术研究了稻田沟渠、池塘、湿地等水体的碳、氮稳定同位素组成特征与时空变化。结果表明,水中颗粒性有机物(Particulate organic matter,POM)δ13C值(稳定性碳同位素比值)在-31.5‰~-24.3‰之间变化,平均值为-27.7‰,可能主要来自于浮游植物和浮游动物的贡献。POM稳定性碳同位素比值存在明显的季节变化,呈现出春、夏季高于秋、冬季的趋势。浮游动物与POM稳定性碳同位素比值之间的时空变化存在一定的相关性,说明研究区内浮游动物对内源有机碳的利用可能主要来自POM。颗粒性有机物δ15N存在秋、冬季高于春、夏季的趋势,但空间差异不显著,其中湿地的变化幅度相对较大(3.2‰~6.3‰),δ15NPOM平均值为4.1‰,说明研究区固氮作用较小,外源物的污染程度较低。     

Is Discrimination of 13C in Potato Leaflets and Tubers an Appropriate Trait to Describe Genotype Responses to Restrictive and Well‐Watered Conditions?

Selection for drought tolerance entails prioritizing plant traits that integrate critical physiological processes occurring during crop growth. Discrimination against ¹³C (?) in leaflets (?_leaflet) and tubers (?_tuber) was compared under two water regimes in two potato‐improved varieties selected to maintain yield under drought conditions (Unica and Sarnav) and one drought susceptible European cultivar (Désirée). In the control treatment, soil water content was kept at field capacity over the whole growth cycle, while in the drought treatment water supply was restricted after tuber initiation (50 % of field capacity). Gas exchange and N content per unit leaf area (N_area) as well as ? were assessed at different stages. Sarnav showed the highest tuber yield in both water conditions, suggesting that yield in the water restriction treatment was largely driven by yield potential in this genotype. Higher stomatal conductance (g_s) and N_area and lower ?_leaflet in well‐watered Sarnav suggested higher photosynthetic capacity. Under water restriction, Sarnav maintained higher g_s indicating that carbon diffusion was a key factor for biomass accumulation under water restriction. Our results suggest the use of ? determined after tuber initiation as an indirect selection indicator for tuber yield under both well‐watered and restricted soil water availability conditions.     

基于稳定同位素的江苏近海金乌贼摄食生态研究

为进一步掌握江苏近海金乌贼(Sepia esculenta)种群生活史特征，根据在江苏沿海港口所采集的样本，利用碳氮稳定同位素分析法对金乌贼的摄食生态及营养生态位进行了研究。结果表明，金乌贼雌、雄群体间 13C和 15N值均不存在显著性差异；金乌贼的 值与胴长之间相关性不显著， 值与胴长之间存在显著相关性；雄性金乌贼营养生态位宽度（SEAc=0.57‰2）与雌性（SEAc=0.63‰2）无明显差异，雌性略大于雄性，且两者高度重叠（0.62）。同位素聚类分析显示，可将金乌贼个体分为75~114mm、115~184mm和185~204mm三个胴长组；185~204mm胴长组营养生态位宽度最大（SEAc=1.77‰2），75~114mm胴长组营养生态位宽度最小（SEAc=0.39‰2）；仅75~114mm和115~184mm胴长组间生态位重叠率为中度重叠（0.34），其余胴长组间生态位重叠率低。分析认为，金乌贼δ13C值的变化主要受同位素基线值和金乌贼洄游模式影响，而δ15N值的变化主要受金乌贼摄食作用影响；雌性和雄性金乌贼群体之间食物资源和栖息地利用高度相似；不同发育阶段的金乌贼在营养生态位上具有明显差异。     

水分和热胁迫处理对4种针叶树苗木气体交换和水分利用效率的影响

以杉木、马尾松、花旗松和北美乔柏为研究对象 ,研究了水分和热胁迫对生长期间苗木的气体交换、瞬间水分利用效率和稳定碳同位素组成 (δ1 3C值 )等生理生态指标的影响 ,结果如下 :(1 )水分胁迫使杉木、马尾松、花旗松和北美乔柏 4个树种的净光合速率显著下降。但热胁迫只影响杉木和北美乔柏的净光合速率 ,对马尾松的净光合速率影响不大。 (2 )对针叶气孔导度的研究发现 ,水分和热胁迫均对马尾松苗木针叶气孔导度有显著影响 ,但对杉木和北美乔柏苗木针叶的气孔导度只有轻微影响。 (3)水分和热胁迫处理对马尾松苗木的瞬间水分利用效率有显著影响 ,但对杉木和北美乔柏的瞬间水分利用效率无显著影响。 (4 )水分胁迫处理对杉木、马尾松、花旗松和北美乔柏 4个树种的稳定碳同位素组成 (δ1 3C值 )均有显著影响 ,随着水分胁迫程度的加深 ,4个树种苗木针叶的δ1 3C值增大 (负值绝对值变小 ) ,说明其永久水分利用效率更高。 (5 )热胁迫对杉木和北美乔柏苗木针叶的δ1 3C值有显著影响 ,且在热胁迫下 ,两树种苗木针叶的δ1 3C值减小 (负值绝对值增大 ) ,即永久水分利用效率下降。但热胁迫对马尾松和花旗松苗木的δ1 3C值没有显著影响     

利用黄土中碳同位素推断古植被类型

黄河中游黄土高原生态环境十分脆弱,已成为中国乃至世界林业、农业、地质和环境专家关注的热点地区.黄土地层中保存着大陆上最完整、最连续的古气候记录(顾兆炎,1991).黄土沉积以黄土层和古土壤层交互沉积为特征.学者们已从各个角度来挖掘这些记录,以探寻最好的气候指标,提取可靠的古气候、古植被信息.     

Nitrogen contribution by multipurpose trees to rice and cowpea in an alley cropping system in Sierra Leone

In an alley cropping experiment, a study was carried out on N₂ fixation by Gliricidia sepium, nitrogen (N) accumulation by prunings of Gliricidia, Senna siamea (formerly Cassia siamea) and Gmelina arborea, and the N contribution to associated crops of rice and cowpea.Total N accumulated by the hedgerow trees ranged from 297–524 kg N ha^–1 on average but varied between tree species and depended on the growing season. Gliricidia sepium accumulated 370 kg N ha^–1 on average and more than half of this came from fixation. Senna siamea and Gmelina arborea served as reference trees for estimating N₂ fixation. The estimates of N₂ fixation using Gmelina as a reference gave higher estimates than those using Senna.Although the dry matter and nitrogen yields of prunings from the hedgerow trees were high, their relative nitrogen contribution to the associated crops was generally low ranging from 5 to 29%. Higher crop yields and nitrogen contribution were observed with Gliricidia sepium prunings. The low N contribution from prunings was attributed to the lack of synchronization between the N released from the prunings and the crop's demand for N.