Detection of the Defoliating Pathotype of Verticillium dahliae in Infected Olive Plants by Nested PCR

Spread of Verticillium wilt into newly established olive orchards in Andalucía, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to new areas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPD marker of the D pathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants.     

广西畜禽现代生态养殖场认证存在问题及对策

为深化生态文明建设成效,推动生态养殖业的整体发展,广西自2016年开始实施畜禽现代生态养殖场认证工作,要求2020年全区90%规模养殖场都需要与现代生态养殖标准所符合。目前广西畜禽现代生态养殖场认证工作成效显著,在较大程度上推动现代生态养殖业的整体发展。但依然有一些问题存在于畜禽现代生态养殖场认证工作中,相关部门及人员需加强重视,及时探索针对性的对策。     

有机认证新标准的解析及对我国有机蔬菜产业的影响

最近发布实施的有机产品认证新标准对有机产品认证提出了更加严格的要求。通过对新标准的解析,系统阐述了新标准对有机蔬菜在生产资料选择、生产过程中的轮作、产品的检测、销售和质量追溯体系建立等方面的要求,分析了新标准对今后我国有机蔬菜产业发展的影响。     

Cortical monoterpene variation in natural populations of Pinus sylvestris in Sweden

Cortical oleoresins were obtained from 10 natural populations of Pinus sylvestris L. from different geographical localities. Gas chromatography was used to determine quantities of individual monoterpenes. Considerable variation was found in monoterpene composition among different populations. High proportions of limonene occurred in greater percentages in trees of northern origins, while high sabinene was frequently found in southern trees. Mean proportions of limonene and sabinene in the populations were clinal with latitude. For some monoterpenes, variation coefficients as a measure of variability within populations appeared to be clinal with latitude. 85.4% of the variance in monoterpene composition was found within populations and 14.6 between populations. Discriminant analysis differentiates populations from different geographical localities into different groups. The differences in monoterpene patterns between adjacent populations were statistically nonsignificant. It is recommended that monoterpene composition be used for more general study of genetic variation, geographical distinction between different populations and seed certification purposes.     

浅伏谈伏HAC伏CP体伏系伏认证伏在肉伏鸡伏屠宰加工中的应用

HACCP认证能有效控制食物中的危害，保证食品的安全，是世界范围上公认的食品安全生产准则。目前，HACCP在各行业中得到广泛应用，尤其在服务业、餐饮业、食品加工业等，但在肉鸡等动物屠宰加工业应用的还不多。本文主要介绍了HACCP体系认证在肉鸡屠宰加工中的应用。     

森林认证与中国木材产品市场

森林认证作为促进森林可持续经营的一种市场机制已经在世界范围内广泛开展 ,特别是欧洲和北美国家的消费者普遍要求在市场上销售的木材产品应贴有经过认证的标签 ,以证明他们所购买的木材产品源自可持续经营的森林。因此 ,中国的森林经营认证和林产品的产销监管链认证 (绿色标签 )对于促进森林的可持续经营 ,稳定企业现有产品市场份额 ,进入新市场具有重要意义。     

人工林可持续经营认证标准与指标问题

阐述了森林认证对于人工林可持续经营的必要性。依据黑龙江森工林区人工林经营实践和森林认证在该地区的发展情况，讨论了FSC原则和标准框架下本区域森林可持续经营认证的人工林标准与指标问题。认为标准与指标应该针对本区域天然林破碎严重，人工林树种单一、稳定性差、生长量低、生态效益不好等一系列问题，提出了人工林标准与指标的基本内容。     

Experiences of a Forest Owners’ Cooperative in using FSC forest certification as an environmental strategy

Yusuhara Forest Owners’ Cooperative (YFOC) in Kochi Prefecture in Japan received its forest management certification from the Forest Stewardship Council (FSC) in 2000. YFOC is recognised as the resource manager of its members’ forests, and they were the first organisation to gain such certification in Japan. With the continuous efforts of selling FSC certified wood in the domestic housing construction market, YFOC has substantially increased their timber sales in recent years. It is a noteworthy event in Japanese small-scale forestry, which has been struggling with declining economic performance for many years. This paper analyses the detail of forestry activities and changes which occurred in YFOC after certification by FSC. An interesting effect of increasing economic performance is found to have come from an unexpected direction. It is concluded that the FSC certification system is a possible tool to revitalise Japanese small-scale forestry as well as obtain favourable environmental outcomes.     

FSC与ISO森林认证体系比较及在我国适应性研究

本文针对当前世界上使用最为广泛的两种森林认证体系—ISO14 0 0 0和FSC进行了多方面比较分析 ,并对这两种认证体系在我国适应性进行了研究。结果表明 ,就目前我国森林经营实际而言 ,进行大面积基于业绩标准的FSC森林认证的条件尚未成熟 ,建议进行基于进程标准的ISO14 0 0 0森林认证 ,这样能够更好地提高我国森林经营水平 ,从而实现森林的可持续经营