牛皮肤成纤维细胞的体外培养与冻存

利用牛皮肤组织块直接培养法，得到牛皮肤细胞的原代培养物，再用酶消化法和反复贴壁法处理，能够纯化成纤维细胞。成纤维细胞的冻存是通过选用6种分别含有二甲基亚砜（DMSO）、甘油（GL）及乙二醇（EG）的保护液，以相同的冻前处理方法，对牛皮肤成纤维细胞进行缓慢冷冻，冰箱预冷平衡1－2h，逐步投入液氮（－196℃）中保存，再经37℃水浴解冻，Hanks液脱保护剂，以贴壁率评价冻存效果。结果表明，20％DMSO保护液对牛皮肤成纤维细胞表现出较好且稳定的冷冻保护效果，其平均贴壁率达87．9％。     

Suppurative Inflammation of Apocrine Sweat Glands (Suppurative Hidradenitis) in the Dog: A Retrospective Clinicopathological Analysis of 100 Cases

Résumé— Une analyse rétrospective clinicopathologique a été menée chez 100 chiens présentant une hydradénite suppurative diagnostiquée par biopsie cutanée. Aucune prédisposition d'âge, de race ou de sexe n'a été mise en évidence. Tous les chiens présentaient cliniquement une folliculite bactérienne, ou une furonculose, ou les deux, qu'elle soit primaire ou secondaire à d'autres dermatoses. Aucene lésion clinique n'était pathognomonique d'hidradénite suppurative. Chez 73 chiens, l'hidradénite suppurative était associée microscopiquement à des degrés variables d'inflammation du follicule pileux. Chez 27 chiens, l'hidradénite suppurative était la seule évidence histologique d'inflammation annexielle. L'observation histopathologique d'hidradénite suppurative suggère l'existence sur le plan clinique d'une folliculite bactérienne, d'une furonculose, ou des 2. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradentis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflammation suppurative des glandes sudorales apocrines (hidradénite suppurative) chez le chien: une analyse rétrospective clinicopathologique de 100 cas). Resumen— Se llevó a cabo un estudio clinicopatológico retrospectivo en 100 perros con hidradenitis supurativa diagnosticada mediante biopsia cutánea. No se detectó predominancia alguna de raza, sexo o edad. A todes los perros se les habia diagnosticado clinicamente foliculitis o furunculosis bacteriana, o ambas, ya sea de forma primaria o secundaria a otras dermatosis. No se encontraron lesiones clinicas especificas indicativas de hidradenitis supurativa. En 73 animales, la hidradenitis supurativa estaba asociada con varios grados de inflamación folicular a nivel microscópico. En los 27 casos restantes, los anejos presentaban unicamente hidradenitis supurativa. La presencia de hidradenitis supurativa a nivel microscópico sugiere la existencia de foliculitis o furunculosis bacteriana clinicas, o de ambas. [Scott, D.W. Suppurative inflammation of apocrine sweat glands (suppurative hidradenitis) in the dog: a retrospective clinicopathological analysis of 100 cases (inflamación supurativa de las glándulas sudoriparas apocrinas (hidradenitis supurativa) en el perro: estudio clinicopatológico retrospectivo de 100 casos). Abstract— A retrospective clinicopathological study was conducted on 100 dogs with suppurative hidradenitis as determined by skin biopsy. No apparent age, breed, or sex predilections were recognized. All dogs had been given a clinical diagnosis of bacterial folliculitis, furunculosis, or both of these, whether primary or secondary to other dermatoses. There were no clinical lesions that uniquely suggested the presence of suppurative hidradenitis. In 73 dogs, suppurative hidradenitis occurred in conjunction with varying degrees of hair follicle inflammation microscopically. In 27 dogs, suppurative hidradenitis was the only histological evidence of adnexal inflammation. The histopathologic finding of suppurative hidradenitis suggests the existence of clinical bacterial folliculitis, furunculosis, or both of these.     

蝴蝶兰组织培养中的褐化控制研究

 系统研究了吸附剂(活性炭、PVP) 与柠檬酸、半胱氨酸、谷胱甘肽、维生素C和硫代硫酸钠5种抗氧化剂对蝴蝶兰组培中褐化的影响。结果表明: 活性炭能有效控制褐化和促进生长, 但不利于分化; 谷胱甘肽控制褐化的效果虽不及活性炭, 但综合效果最佳。     

激素和蔗糖浓度组合对蕨菜愈伤组织诱导的影响

以蕨菜[Pteridium aquilinum(L.)Kuhn]的幼嫩叶柄为外植体,分别用两种褐变抑制剂(柠檬酸和抗坏血酸)对外植体进行预处理,并在其愈伤组织诱导培养基中设置不同的激素、吸附剂聚乙烯吡咯烷酮(PVP)和蔗糖浓度,研究了不同处理对蕨菜外植体褐变及愈伤组织诱导的影响。结果表明:抗坏血酸的抗褐变效果优于柠檬酸,在培养基中添加PVP可以增强这两种褐变抑制剂的抗褐变作用;2.2 g/L PVP+30 g/L蔗糖+1/2MS+NAA 0.4 mg/L+6-BA 0.2mg/L为诱导蕨菜愈伤组织的最佳培养基,愈伤组织诱导率为48.3%。     

Photodynamic therapy of feline cutaneous squamous cell carcinoma using a newly developed liposomal photosensitizer: preliminary results concerning drug safety and efficacy

BACKGROUND: Squamous cell carcinomas are common skin tumors in cats. We investigated photodynamic therapy (PDT) using a new liposomal photosensitizer as a minimally invasive, effective treatment that can be easily performed while achieving good cosmetic results. AIM: The goal of this study was to assess and describe possible toxicities using a liposomal formulation of the photosensitizer meta-(Tetrahydroxyphenyl)Chlorin (m-THPC) and investigate if favorable pharmacokinetics translate into favorable tumor response and control. ANIMALS: Eighteen client-owned cats with 20 spontaneous cutaneous squamous cell carcinomas were included in the study. METHODS: PDT was performed using a new, liposomal formulation of the photosensitizer. Toxicity, tumor response, and tumor control were evaluated retrospectively. RESULTS: No general adverse effects were observed in cats treated with the new liposomal formulation. Mild local toxicity such as erythema and edema were seen in 15% of the patients. All cats responded to therapy, with a complete response rate of 100%. The overall 1-year control rate was 75%. The tumor recurrence rate was 20% with a median time to recurrence of 172.25 +/-87.1) days. CONCLUSIONS AND CLINICAL IMPORTANCE: A new liposomal photosensitizer was successfully used for squamous cell carcinoma in cats and was well tolerated. There were no systemic adverse effects observed with the liposomal formulation. The favorable pharmacokinetics of the liposomal drug resulted in a favorable tumor response.     

杨树烂皮病药剂筛选及防治技术

对杨树烂皮病的化学防治药剂进行筛选结果表明，可杀得（53．8％干悬浮剂）、甲基硫菌灵（50％可湿性粉剂）、双效灵（10％）有明显的抑菌效果；可杀得（53．8％干悬浮剂）、甲基硫菌灵（50％可湿性粉剂）、双效灵（10％）3种药剂毒力线的回归关系显著。对8种杀菌剂进行杨树烂皮病防治试验的结果表明，可杀得等杀菌剂防治效果达90％，药效显著。     

安徽白山羊杂交效应

对安徽白山羊及其二元杂交组合(波×安,萨×安)、三元杂交组合(波×萨×安,马×萨×安)和级进杂交组合(波×波×安)研究表明:(1)杂交能提高安徽白山羊的生长发育速度。二元杂交和三元杂交组合在6月龄前生长较快,6月龄后变慢;级进杂交在8月龄前生长较快。(2)无论是二元杂交还是三元杂交,均能提高安徽白山羊的产肉性能(P<0 01,P<0 05),但肉质有下降的趋势,三元杂交比二元杂交下降更多。(3)二元杂交和三元杂交能够增大安徽白山羊板皮面积(P<0 01,P<0 05),减小毛纤维密度,二元杂交使板皮的均匀度提高。