Non-steroidal anti-inflammatory drugs induce apoptosis in implanted hepatoma of mice

AIM: To study the mechanism of the effect of NSAIDs on apoptosis in mice hepatoma at anti-inflammatory doses. METHODS: Kunming breed mice were inoculated subcutaneously in the flank with mice hepatoma H22 cell line. The effects of ibuprofen, indomethacin, and nimesulide on apoptosis were determined by using electron microscopy, agarose gel electrophoresis, flow cytometry, and Western blot analysis of the expression of c-myc, bcl-x_L and bcl-2 proteins. RESULTS: NSAIDs induced apoptosis of mice implanted hepatoma, which includes the morphological changes such as reduction in the volume, and the nuclear chromatin condensation, as well as the "ladder pattern" revealed by agarose gel electrophoresis of DNA. The apoptotic index was increased to 15%±1.0%, 29.7%±1.5%, 46.3%±3.5% from 3.3%±0.6% by detecting Sub-G₁ peaks on flow cytometry. Western blot analysis showed that levels of bcl-2 and bcl-x_L were significantly reduced by treatment with nimesulide. Ibuprofen and indomethacin decreased bcl-2 expression but increased bcl-x_L expression. C-myc wasn't changed in these groups. CONCLUSION: These results suggest that NSAIDs induces apoptosis of mice hepatoma, which may be due to their regulation on the expressions of bcl-2 family genes.     

桑树树液蛋白质的双向电泳分析

应用 Ｉ Ｅ Ｆ 和 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 双向电泳技术，分析了桑树（ Ｍｏｒｕｓ） 树液蛋白质的电泳图谱。结果表明，供试栽培品种的蛋白质谱比较相似，相似系数在０７２ ～１００ 之间；品种间存在１４ 个共有组分，占检测组分总数的５１９ ％ ；桑树栽培种与野生种的差异比较显著。     

天然雌核发育普安鲫(A型)细胞遗传学和血清电泳的初步研究

本文对贵州省普安县青山一带天然水体中的普安鲫(A型)作了细胞遗传学观察和血清电泳分析,发现它是天然雌核发育的鲫鱼,既不同于普通野鲫,也与已知的天然雌核发育方正银鲫和滇池高背鲫不是同一来源。     

Associations between bacterial genotype and outcome of bovine clinical Staphylococcus aureus mastitis

Background

Staphylococcus aureus is an important cause of clinical mastitis in dairy cows worldwide. The cure rate after antimicrobial treatment of clinical S. aureus mastitis is very variable due to both cow and bacterial factors. Studies have shown that bacterial genotype might affect short-term bacteriological and clinical cure, but the long-term outcome has been less studied. The objectives of this study were to investigate associations between bacterial genotype and long-term outcome of veterinary-treated clinical mastitis (VTCM) caused by S. aureus during a follow-up period of 120 days and to study genotype variation among Swedish S. aureus isolates. S. aureus isolates from cases of VTCM were genotyped by pulsed-field gel electrophoresis. Long-term outcome measurements used were somatic cell count (SCC), additional diagnoses of VTCM, milk yield and culling. Isolates were classified into clusters (>80% similarity) and pulsotypes (100% similarity). Clusters and pulsotypes were grouped according to occurrence. Multivariable mixed-effect linear regression models including cow and bacterial factors with possible influence on SCC or milk yield were used to calculate differences in SCC or milk yield between groups. Additional outcome measures were calculated using a test of proportions.

Results

The isolates (n = 185) were divided into 18 clusters and 29 pulsotypes. Two pulsotypes were classified as common, and were found in 64% of the cases of VTCM. Remaining isolates were classified as less common or rare pulsotypes. The distribution was similar at cluster level. Outcome was calculated from follow-up data on 111 cows. Significantly lower SCC during the follow-up period was found in cows infected with common clusters compared to in cows infected with less common/rare clusters. The proportion of cows with SCC <200 000 cells/ml during the whole follow-up period was significantly higher in the group common clusters than in the group less common/rare clusters. Bacterial genotype did not influence the other outcome parameters.

Conclusions

In Sweden, two S. aureus pulsotypes, identified in about 64% of clinical S. aureus cases, were widespread. Cows infected with the common genotypes had significantly lower SCC during 120 days after treatment compared to cows infected with less common or rare genotypes.     

Paraoxonase activity as a tool for clinical monitoring of dogs treated for canine leishmaniasis

This study was designed to determine if the activity of paraoxonase (PON1), an antioxidant enzyme that works as a negative acute phase reactant, is a better predictor for the clinical recovery of leishmaniotic dogs receiving standard treatments compared with inflammatory markers such as C reactive protein (CRP) and electrophoretic fractions. For this purpose we tested 20 healthy dogs (controls) and 39 leishmaniotic dogs classified as sick (group A, n = 23) or severely sick (group B, n = 16) and tested at admission and after 3, 7, 14, 21, 28, 35 and 42 days.At admission, CRP and electrophoresis were altered in both groups, while PON1 activity was abnormal only in group B. There were no differences related to the outcome (mortality, complications or time of recovery). PON1 activity normalized in about 2 weeks in dogs that had abnormal values at admission and a final positive outcome; CRP normalized in 4–6 weeks and electrophoretic fractions were still altered after 6 weeks. The results show that, at admission, inflammatory markers did not predict the outcome of leishmaniasis. PON1 activity decreased only in some dogs with systemic inflammation but not in those with mild leishmaniasis: when decreased, PON1 normalized earlier than other markers in dogs that responded to treatment. This finding most likely depends on the rapid decrease in oxidative phenomena. PON1 activity should therefore be tested on admission: if low values are recorded, severe inflammation may be suspected and PON1 measurement may be repeated during treatment to early identify responsive dogs.     

不同基因型冬小麦花药出愈率及其愈伤组织的蛋白质电泳分析

用不同基因型的冬小麦品种花药在Ｗ１４２培养基（改进的Ｗ１４培养基）上获得较高的出愈率，但不曲因型之间仍存在着显著的差异。用Ｐｈａｒｍａｃｉａ公司ＰｈａｓｔＳｙｓｔｅｍ电泳仪对不同基因型小麦花药及其愈伤组织蛋白质进行等电聚焦电泳和ＳＤＳ－聚丙烯酰胺凝胶电泳分析，观察到不同基因型花药蛋白质之间存在着差异，并且初步认为这种蛋白质差异与出愈率有相关性。不同基因型花药愈伤组织之间观察不到明显的带型区别，但它     

山羊乳蛋白多态性与产奶性能的关系研究

利用聚丙烯酰胺凝胶电泳分析２２５只西农萨能奶山羊乳样，首次对山羊乳中主要蛋白质的多态性与乳成分、产奶量等产奶性能指标的关系进行探讨。结果表明，β－乳球蛋白的基因型对奶山羊产奶量有显著影响，而α－酪蛋白、ｋ－酪蛋白对产奶性能无显著影响作用，β－乳球蛋白ＢＢ型、α－酪蛋白ＢＢ型和ｋ－酪蛋白ＡＡ型山羊个体表现出较好的产奶性能。     

糯小麦及其Waxy基因的研究进展

小麦的淀粉分为直链淀粉和支链淀粉,在控制直链淀粉合成的酶中有一种关键的酶,称为Waxy蛋白,Waxy蛋白有3个基因位点,即Wx-A1、Wx-B1和Wx-D1,在六倍体小麦中它们分别位于7A、4A和7D染色体上,当这3个位点的基因都缺失时,小麦就呈现糯性,糯小麦是指不含直链淀粉或者直链淀粉含量很低的小麦品种,它对小麦的淀粉以及面团等特性都有很大的影响,并且在食品和非食品工业中也有着重大的用处,所以在近几年来出现了糯小麦的研究和培育的热潮,研究者利用蛋白质电泳技术鉴别出部分突变的品系,并利用分子标记及其他手段人工培育出了糯小麦;随着研究的深入,研究者发现了Waxy基因的新的突变;对以上的问题进行了综述,并对其中的部分问题作了分析和展望。     

THE STUDY ON ESTERASE AND PEROXIDASE ISOZYMES OF WHEAT LANDRACES FROM SHAANXI, CHINA

<正> The esterase and peroxidase isozymes of 63 landraces of wheat (Triticum aestivum L.) from Shaanxi province, China have been studied. The results showed as follows:Significant differences of either esterase or peroxidase isozymes were detected in both roots and shoots of seedling among landraces. E_3 and E_5 were special for roots; E_4, E_(10), E_(11), E_(12) and E_(13) only appeared in shoots. 2 types of isozyme patterns of esterase were involved in roots, but 4 types in shoots. No variation of peroxidase isozymes appeared in roots, but 3 variations arose in shoots. The roots showed more zymograms and more activity than the shoots in peroxidase isozyme.     

Polymorphism and Genetic Diversity for the Seed Storage Proteins in Spanish Cultivated Einkorn Wheat (Triticum monococcum L. ssp. monococcum)

The seed storage protein composition of one collection of cultivated einkorn wheat (Triticum monococcum L. ssp. monococcum) of Spanish origin has been analysed by SDS-PAGE and A-PAGE. Three allelic variants were detected for the Glu-A1^m, whereas up to six alleles were detected for the Glu-A3^m. For the gliadins, 7 and 14 alleles for the Gli-A1^m and Gli-A2^m were found between the evaluated accessions. Internal variability was detected in some of these materials, which could be related to the landrace nature of them. Up to 48 different genotypes based on the origin and seed storage protein composition have been identified. Further researches on these materials must be carried out for determining the variability degree in morphological traits that could complement the evaluation for their safeguard.