水稻苗田化学除草剂—封闭1号大面积苗田药效示范试验结果

通过多点水稻苗田大面积示范试验结果表明,通化市农科所研制的水稻苗田除草剂“封闭1号”具有除草效果好(90～100％)、安全、使用方便、成本低等特点。1988～1991年,累计推广面积(苗田)达15.6万亩,创造经济效益150万元。     

Molecular cloning, expression, and characterization of a phi-type glutathione S-transferase from Oryza sativa

The structural gene for glutathione S-transferase in Oryza sativa was successfully cloned from a cDNA library by the polymerase chain reaction method. The deduced amino acid sequence of this gene showed 44-66% similarity to the sequences of the class phi GSTs from Arabidopsis thaliana and Zea mays. This gene was expressed in Escherichia coli with the pET vector system and the gene product was purified to homogeneity by GSH-Sepharose affinity column chromatography. The expressed OsGSTF3-3 was a homo-dimer composed of 24 kDa subunit and its pI value was approximately 7.3. The OsGSTF3-3 was retained on GSH affinity column and its K_m value for GSH was 0.28 mM. The OsGSTF3-3 displayed high activity toward 1-chloro-2,4-dinitrobenzene, a general GST substrate and also had high activities towards acetanilide herbicides, alachlor, and metolachlor. The OsGSTF3-3 was highly sensitive to inhibition by benastatin A and S-hexyl-GSH. From these results, the expressed OsGSTF3-3 is a phi class GST and seems to play an important role in the conjugation of the chloroacetanilide herbicides.     

以白菜为例的转基因植物环境安全性评价研究

以转除草剂抗性基因的白菜为例,通过对具体事实的展现和分析,介绍了关于转基因植物环境安全性评价的方法,以及目前国际上关于转基因作物的一些比较一致的结论。针对我国的农业生态环境,结合试验结果,讨论了获得的转基因白菜的环境安全性。     

Reduced paraquat translocation in paraquat resistant Arctotheca calendula (L.) Levyns is a consequence of the primary resistance mechanism, not the cause

Resistance to paraquat has been studied in detail in many weed species for more than a decade, with the precise mechanism of resistance still unclear. Several studies have indicated that reduced movement of the herbicide to the site of action in the chloroplast is at least partly responsible for endowing resistance. Although paraquat translocation studies have been performed in the past it has been rare for these studies to have been conducted on whole plants in the light, despite early observations which clearly showed that paraquat translocation is minimal unless treated plants are exposed to light. This study has addressed this issue in Arctotheca calendula by tracing the movement of ¹⁴C-paraquat in resistant and susceptible plants in both the dark and light. Differences in paraquat translocation between the resistant and susceptible biotypes of A. calendula were only observed when treated plants were exposed to light. It was observed that paraquat translocation was significantly reduced in the resistant compared to the susceptible biotype when plants were exposed to light but not in the dark. It is postulated that paraquat translocation is dependent on light mediated damage. As paraquat-induced damage is less severe in paraquat resistant plants, overall paraquat translocation is reduced in the resistant biotype.     

A mutation confers Monochoria vaginalis resistance to sulfonylureas that target acetolactate synthase

Acetolactate synthase (ALS) is the target enzyme for four distinct families of compounds: sulfonylureas (SUs), imidazolinones, triazolopyrimidine sulfonanilides, and pyrimidinyl oxybenzoates. We cloned and sequenced the fragments encoding ALS genes from biotypes of Monochoria vaginalis susceptible (S) and resistant (R) to SU-herbicides. The nucleotide sequences of the 39 bp Domain A region for R M. vaginalis biotype differed from that of the S biotype by a single nucleotide substitution at variable Pro codon of Domain A (CCT to TCT), predicting a Pro in the S but a Ser in the R biotype. No nucleotide differences between S and R M. vaginalis were observed in Domain D. We suggest that the amino acid substitution at Domain A region is responsible for resistance to SU-herbicides in M. vaginalis collected from Ushiku City, Ibaraki Prefecture, Japan.     

Biology,impact, and management of Echinochloa colona (L.) Link

Echinochloa colona (L.) Link. is one of the most problematic weeds across the world. It is an annual C₄ summer grass, native to tropical and subtropical Asia, emerged as a serious and persistent threat in 35 cropping systems in more than 60 countries. E. colona is reported as an important associated weed species in transplanted and direct-seeded rice. Diverse ecotypes, high seed production, short seed dormancy, rapid growth, competitive potential, allelopathic interaction, and resistance against several herbicides makes it a more adaptable and persistent challenge in various agro-ecosystems. Development of resistance to recommended or higher doses of numerous herbicides, including ametryn, atrazine, bispyribac-sodium, clefoxidym, cyhalofop-butyl, fenoxaprop-p-ethyl, glyphosate, metribuzin, propanil, and triazine, is a serious concern for the farming and scientific community. Crop infestation with resistant E. colona biotypes may ultimately increase the weed control cost. Unfortunately, investigations on seed dormancy release, genetic diversity, allelopathic interference, and competitive ability of this weed are inadequate in accomplishing its appropriate control in different environments. Therefore, a comprehensive review is presented here to gather the existing information, to pin point key findings, and to highlight the research gaps in the biology, interference, and management of E. colona. Different management options have been discussed in relation with eco-biology of this noxious weed. The potential research endeavours have also been highlighted in order to provide an insight of its existing scenario and to facilitate the future management strategies.     

Agricultural practice changes with cultivating genetically modified herbicide-tolerant oilseed rape

To facilitate weed suppression, oilseed rape or canola (Brassica napus) has been genetically modified (GM) over the last two decades to incorporate herbicide tolerance (HT). The introduction of oilseed rape in commercial agriculture raises concerns about potential adverse agronomic and environmental effects linked to both the genetic modification and altered agricultural practice. How will this new crop modify the existing cropping practice? What are the potential agro-environmental implications of its introduction? This paper analyses and schematises recorded and conceivable agricultural practice changes with GMHT oilseed rape introduction and its likely agro-environmental effects. It develops a conceptual systematics of influencing factors, subsequent practice changes and likely agro-environmental effects. The results can be used to design the mandatory monitoring of adverse GM crop effects.     

Herbicide savings and economic benefits of several strategies to control Sorghum halepense in maize crops

This study was conducted to assess the herbicide savings and the cost efficiency of site-specific herbicide application strategies in comparison with other strategies based on uniform application of herbicides throughout the whole field. The specific situation considered was Sorghum halepense infested maize fields in Spain. The results from a theoretical economic model were contrasted with the information derived from a S. halepense survey conducted in 37 commercial maize fields distributed over three Spanish maize production areas. Seven application strategies were simulated: 1) no herbicide; 2) overall full-rate; 3) overall half-rate; 4) and 5) site-specific spraying infested cells with full- and half-rate, respectively; 6) and 7) site-specific spraying infested cells plus adjacent buffer areas with full- and half-rate, respectively. The simulation results showed that site-specific weed management was the most profitable strategy when S. halepense infested area ranged between 6.5 and 18.7%. This scenario was present in 22% of the surveyed fields. In fields with less than 6.5% infestation (a situation present in 51% of the surveyed fields), yield losses were slight and the most profitable strategy was using no herbicide. When the infested area ranged between 18.7 and 40.8% (19% of the surveyed fields), no significant differences were observed between the net benefits of the various strategies. Full rate herbicide applications throughout the entire field resulted in the highest net returns in fields with more than 40.8% weed infestation (8% of the surveyed fields). In these cases, the added costs of weed detection, mapping and site-specific herbicide application were not justified by the herbicide savings obtained. Likewise, herbicide savings obtained with the various strategies depended on the proportion of the field infested. Considering the high difference between site-specific treatments and uniform application of herbicides when the infested area ranged between 18.7 and 40.8%, and that no significant differences in net returns were observed in this range, site-specific treatments should be preferred. Adding a buffer area to the site-specific treatments does not seem a suitable strategy due to the considerable increases in costs and reductions in herbicide savings.     

The carboxyterminal processing protease of D1 protein: Herbicidal activity of novel inhibitors of the recombinant and native spinach enzymes

The carboxyterminal processing protease of D1 protein (CtpA) is predicted to be an excellent target for the discovery of a general broad-spectrum herbicide. Directed and random screening of compounds against recombinant spinach CtpA (rCtpA) has led to the discovery of five different chemical classes of inhibitors. Lead compounds from each inhibitor class were investigated for their in vitro effects on the activities of both recombinant and native spinach CtpA. All of the lead compounds have K_i values of less than 50 μM when tested against rCtpA, and all except one showed competitive inhibition. Results from partially purified native CtpA from spinach were similar to those from the recombinant form of the enzyme, thus validating the use of rCtpA in the inhibitor screen. Compounds from three of the classes of CtpA inhibitors show in vivo herbicidal activity against Arabidopsis thaliana when applied either by addition to growth media or by spraying the leaves. Transgenic Arabidopsis plants which over-express CtpA showed greater resistance to the compounds than wild-type plants providing evidence that these inhibitors are directly acting against CtpA.     

Weed control in conservation agriculture systems of Zimbabwe: Identifying economical best strategies

Weed management under conservation agriculture (CA), especially when manually controlled is one of the major setbacks for the widespread adoption of CA in southern Africa. This study was conducted at three on-station and three on-farm sites: CIMMYT-Harare, Domboshawa Training Centre and Henderson Research Station (on-station sites), Hereford farm, Madziva communal area and Shamva communal area (on-farm sites). The evaluation focused on the effect of initial herbicide application and succeeding manual weeding whenever weeds were 10 cm tall or 10 cm in length for grasses with stoloniferous–rhizomatous growth habit. Weeds counts, weeding time and grain yields were collected at all on-station sites. At the on-farm sites, weed counts were done before weeding and a number of farmers were timed during weeding. The results showed that herbicides use reduced the weed density and time taken on weeding at all sites. Combining herbicides e.g. atrazine, glyphosate and metalachlor had the lowest weed density and weeding time at all sites. However, the treatments had no effect on maize grain yields suggesting that appropriate and timely manual weeding reduced crop/weed competition. Herbicides treatments had higher input costs than manual weeding due to the additional cost of herbicide but the treatment with manual weeding only had more overall labour days compared to the mixture of three herbicides. In order to achieve economic benefits, smallholder farmers may use the time for value addition e.g. expand cropped land area, use time for value addition, or sell new products on the market. Herbicides use reduces the manual labour needed to control weeds and minimise total crop failure due to untimely weeding hence, herbicides are an important but not the only weed control option under CA systems in Zimbabwe.