应用FA及PPA-ELISA技术对猪瘟的检测

用免疫荧光抗体诊断技术及单克隆抗体纯化酶联免疫吸附试验诊断技术对疑为猪瘟病毒感染猪进行了检测 ,重点阐述了 2种方法的技术原理和操作过程。通过用免疫荧光抗体诊断技术检测了 5份病料 ,其中有 2份为阳性 ,阳性率为 40 % ;用单克隆抗体纯化酶联免疫吸附试验诊断技术检测了猪瘟血清抗体 ,在 40头母猪血清样品中 ,猪瘟弱毒抗体效价 OD值较高 ,有 1 0 0 %的保护率 ,但是发现母猪群中有 1 0 %隐性猪瘟感染 ,其强毒抗体效价 OD值大于 0 .5,体内带有猪瘟病毒。结果及过程表明此两种诊断方法检测快速、鉴别准确、分辨率高     

FA旱地龙在农作物上抗旱增产效果研究

在粮食、油料、蔬菜、瓜果等多种作物上使用FA旱地龙的结果表明 ,FA旱地龙能促进作物的生长发育 ,增强作物抗寒、抗旱、抗干热风等抗逆性能 ,减轻灾害 ,提高农作物产量。同时筛选出了在不同作物上FA旱地龙的最佳施用方法及施用适期     

Whole wheat bread: Effect of bran fractions on dough and end-product quality

Consumption of whole-wheat based products is encouraged due to their important nutritional elements that benefit human health. However, the use of whole-wheat flour is limited because of the poor processing and end-product quality. Bran was postulated as the major problem in whole wheat breadmaking. In this study, four major bran components including lipids, extractable phenolics (EP), hydrolysable phenolics (HP), and fiber were evaluated for their specific functionality in flour, dough and bread baking. The experiment was done by reconstitution approach using the 2⁴ factorial experimental layout. Fiber was identified as a main component to have highly significant (P < 0.05) and negative influence on most breadmaking characteristics. Although HP had positive effect on farinograph stability, it was identified as another main factor that negatively impacted the oven spring and bread loaf volume. Bran oil and EP seemed to be detrimental to most breadmaking characteristics. Overall, statistical analysis indicates that influence of the four bran components are highly complex. The bran components demonstrate multi-way interactions in regards to their influence on dough and bread-making characteristics. Particularly, Fiber appeared to have a high degree of interaction with other bran components and notably influenced the functionality of those components in whole wheat bread-making.     

百花山山地土壤中有机质的垂直分布规律

本文测定了北京百花山山地典型土壤剖面各发生层的有机质含量、腐殖酸组分、可见光谱,利用相关分析方法计算了有机质与海拔高度之间的相关系数。结果表明:有机质与海拔高度的相关系数为0.94,,呈显著相关水平;各剖面土壤腐殖质分析结果表明,该山地土壤的HA/FA值、E4/E6值随着海拔高度的升高而呈现上升趋势。     

抗鸭副粘病毒单克隆抗体的制备及其特性鉴定

【目的】制备抗鸭副粘病毒(DPMV)的单克隆抗体,为鸭副粘病毒病免疫学诊断方法的建立奠定基础。【方法】采用差速和蔗糖不连续密度梯度离心法提纯DPMV,免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞株SP2/0融合,采用间接ELISA、间接免疫荧光试验(IFA)和血凝抑制(HI)试验等方法筛选阳性杂交瘤细胞株,并测定其生物学特性。【结果】筛选到7株能稳定分泌抗DPMV单克隆抗体的杂交瘤细胞株,分别命名为J28、F4、B3、E39、E41、A12和A30。这7株单克隆抗体都具有ELISA和1FA特性,其中J28和F4还具有HI和中和特性。免疫球蛋白亚类测定结果显示,J28和F4均为IgG1,其他5株均为IgM。相加ELISA试验结果显示,单抗B3,J28与F4,A30与E39/E41/A12分别识别3个不同的抗原位点。特异性测定结果显示,7株单克隆抗体与雏番鸭细小病毒(MPV)、鹅细小病毒(GPV)、鸭肝炎病毒(DHV)、番鸭呼肠孤病毒(MDRV)及正常细胞均无交叉反应。【结论】筛选出了7株具有ELISA、1FA等特性且分泌抗DPMV单克隆抗体的杂交瘤细胞株,并明确了各株单克隆抗体的特性,为鸭副粘病毒病的免疫治疗和临床诊断奠定了基础。     

苏皖南部丘陵茶园土壤的腐殖质组成及性质的研究

本文是对安徽祁门和十字铺、江苏宜兴和深水等地不同肥力水平的茶园土壤腐殖质含量、组成与性质的研究。土壤类型包括黄壤,红壤和黄棕壤。三个地区茶园土壤腐殖质组成都以富里酸为主,不同地区HA/FA值自黄壤向黄棕壤递增,同一地区高产茶园土壤HA/FA值大于低产茶园土壤,胡敏酸光密度值以黄壤最高,红壤居中,黄棕壤最低。     

土壤中水溶性有机质的结构特征及其与富里酸、胡敏酸的比较

采用元素分析、1HNMR和FTIR研究了黄泥土中水溶性有机质组成及结构特征,并与富里酸、胡敏酸进行比较。结果表明:胡敏酸含有大量的芳香族不饱和物质,烷基链烃多,支链长;富里酸以含有大量的羧基为主要结构特征;水溶性有机质主要由碳水化合物组成,含有大量的羟基,芳香族不饱和物质少。     

硒锌和富啡酸配施对紫花苜蓿叶片抗氧化酶活性及产量的影响

在氮磷钾供应充分情况下,采用大田小区试验方法,通过播前基施硒（Se）、锌（Zn）微肥和腐殖酸（富啡酸,fulvic acid,FA）,设对照（CK,不施肥）、施氮磷钾（NPK）、增施富啡酸（NPK+FA）、增施锌、硒微肥（NPK+Zn+Se）、增施富啡酸和锌、硒微肥（NPK+FA+Zn+Se）等5个不同施肥处理,在严重缺硒和锌石灰性土壤中,研究了硒锌和富啡酸配施对紫花苜蓿叶片内过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）等抗氧化酶活性、游离脯氨酸（Pro）及丙二醛（MDA）和干草产量的影响。结果表明：两茬中叶片过氧化物酶（POD）活性均以NPK+FA处理最高,其次是NPK+FA+Zn+Se处理,说明富啡酸（FA）比微肥（Se+Zn）更能有效提高紫花苜蓿叶片过氧化物酶活性,而硒锌对其活性影响不显著;硒锌与富啡酸配施可显著提高紫花苜蓿叶片内游离脯氨酸（Pro）含量、过氧化氢酶（CAT）及超氧化物歧化酶（SOD）活性,从而显著提高紫花苜蓿抗氧化能力;但各施肥处理对丙二醛（MDA）无显著影响。施肥可显著提高紫花苜蓿产量,施用富啡酸比硒锌微肥的增产效果更明显,且硒锌与富啡酸配施效果最好。因此,在氮磷钾供应充足条件下,适量硒锌微肥或富啡酸均能显著提高紫花苜蓿的抗氧化能力及提高紫花苜蓿产量,且微肥和富啡酸配施效果最好。     

The development and comparison of bio-thermoset plastics from epoxidized plant oils

This study investigates the development of thermoset plastics from plant-based oils (e.g., linseed, soybean, cottonseed, oilseed radish, and peanut oils) using an optimal process of solvent-free epoxidation. The epoxidation of plant oils can be accomplished economically by reacting the double bonds of fatty-acids with hydrogen peroxide. During the solvent-free process catalyzed by the ion-exchange resin, we observed that the influence of several variables was important: the molar ratio of hydrogen peroxide to unsaturation, acetic acid to unsaturation, and temperature. The epoxidation of plant oils was determined from the liquid mixture and the composite matrix by thermal and spectroscopic analyses. Compounds with a higher double-bond (iodine) value showed higher oxirane oxygen percent and selectivity, and a higher hydroxyl value because of a greater possibility of attack by solutions causing side reactions. Lower iodine values indicated fewer epoxy groups and selectivity, and a lower hydroxyl value. Benzyl pyrazinium hexafluoroantimonate (BPH) yielded good thermal curing properties; as little as 1% added to the plastics produced light-weight composites. Epoxidized linseed oil promises the highest modulus and impact resistance due to the largest number of double bonds to contribute more epoxy groups and the large proportion of linolenic acids to produce epoxy groups rapidly.     

秸秆还田模式对土壤有机碳及腐植酸含量的影响

通过9年水稻-小麦轮作田间定位试验,探讨南方稻麦两熟制农田秸秆还田模式对土壤有机碳(SOC)和腐植酸(HE)的影响。试验设置仅麦秆稻季还田(W)、仅稻秆麦季还田(R)、秸秆稻麦季均还田(RW)和秸秆均不还田(CK)共4个处理。结果表明,秸秆还田显著(P0.05)提高了0~10 cm土层SOC,对土壤总氮(TN)无明显影响;不同秸秆还田模式处理下,0~10 cm土层SOC及TN大小为WRWRCK;10~20 cm SOC及TN大小均为WCKRWR,但各处理之间的差异均不显著。秸秆还田处理中,0~10 cm土层土壤HE、富里酸(FA)和胡敏酸(HA)均低于CK,而在10~20 cm土层则高于CK。不同秸秆还田方式间,0~10 cm土层的HE、FA和HA以W处理为最高,其土壤腐殖化程度最大;而10~20 cm土层则以RW处理为最高,W处理的土壤腐殖化程度最小。相比其他秸秆还田模式,麦秸稻季还田能更好地提高土壤表层有机碳含量和腐殖质品质。