Vitrification of mouse two-cell and blastocyst stage embryos in simplified closed system using either a hemi-straw or a hollow fiber device

Two-cell stage and blastocyst stage mouse embryos were equilibrated in a medium containing 7.5% ethylene glycol (EG) and 7.5% dimethyl sulfoxide (DMSO) for 8–15 min. Vitrification was performed in a medium containing 0.5 M sucrose and either 15% EG + 15% DMSO, 17.5% EG + 17.5% DMSO, or 20% EG + 20% DMSO for 30 s. They were then placed either on a hemi-straw (HS) or a hollow fiber vitrification (HFV) device and vitrified by cooled air inside a 0.5-ml straw. In two-cell embryos, a 100% survival rate was obtained from all groups except the 20% HS group (P > .05). All vitrified two-cell groups showed similar rates of blastocyst development to that of fresh control group (P > .05), except 17.5% and 20% HFV groups, which were significantly lower than the other groups (P < .05). In the blastocyst embryos, the HFV groups were divided into two subgroups (non-collapsed; HFV-NC and collapsed; HFV-C blastocyst). Re-expansion rate in 15% HFV-NC, 17.5% HFV-NC, and 15% HFV-C groups was reduced (P < .05), whereas the rest were similar to control. In conclusion, we established a simplified, reliable, and closed system for HFV vitrification applying hemi-straw, which does not require skilled practitioners.     

河南省承接产业转移的现状和特点

本文就河南省承接产业转移的需要,把快速调整产业结构、实现跨越式发展的现实,区域间的产业梯次分布转移的前提,河南省承接产业转移的时机条件,承接产业转移的势头,以及面临的差距、面临的竞争形势、区域竞争形势进行了阐述。     

Bubbleless Aerated Membrane Bioreactor for Ramie Degumming Wastewater Treatment

Through introducing the principle of bubbleless aerated membrane bioreactor, the traditional membrane bioreactor was improved. It could be obtained that the removal efficiency of COD and NH3 - N in bubbleless aerated membrane bioreactor increased 4% and 2% than that in traditional membrane bioreactor, respectively. Adopting bubbleless aeration as reflushing air which not only increased DO in the MBR, but also advanced the oxygen utilization, and the energy consumption decreased as well.     

AN APPRONTMATE CALCULATING METHOD FOR WALL PLATES OF MAN'' S DRY GAS HOLDERS

The basic equations of wall plates of MAN' S dry gas holders are derived in this paper. According to the law of cylindrical bending given by the finite element results .we derive some approximate formula calculating the deflection and stresses of the wall plates from the basic equations. Compared with the known approximate calculating method.the method car. obviously increase the percision.     

谈中小桥采用后张法16m预应力空心板的施工问题

本文阐述了中小桥上部结构采用后张法１６ｍ预应力空心板的施工时应注意的问题，如预应力空心板的预制中底模的施工；侧模、蕊模；砼的要求；浇筑空心板的具体步骤。     

具脱层的正交各向异性梁-板的屈曲分析

根据非线性理论，假定脱层为贯穿梁幅的一维脱层，且脱层位置任意，建立了轴向压力作用下含脱层的正交各向异性梁－板的非线性控制方程，对结构进行了分区处理，通过使控制方程适当线性化，利用脱层前缘的连续性条件、平衡条件及相容条件，导出了夹支边界情况下的具脱层的正交各向异性梁－板关于屈曲荷载的无量纲化的特征方程，确定了临界压力荷载，并讨论了脱层尺寸及位置对临界荷载的影响。     

木粉/PVC复合材料挤出流动性的影响因素

研究木粉的粒径、添加剂的种类及配比等因素,对木塑复合材料挤出流动性的影响.结果表明:木粉粒径越小,复合物料在挤出过程中的流动性能越差,所制备的木塑复合材料色差越大,且产品材色越深.综合考虑加工特性和制品材色,宜选用木粉粒径45～60目；并添加稀土热稳定剂及铝酸酯偶联剂,可以提高物料的挤出流动性.     

木质素/PVC复合材料的研究与发展

阐述了将木质素磺酸盐与聚氯乙烯(PVC)熔融挤出制备木质素/PVC复合材料。该产品物理力学性能优于普通人造板,无甲醛释放,可循环再利用,有很好的环境效益。     

非线性阻尼下裂纹非匀速扩展问题的零级近似解析解

探讨了非线性阻尼下无限岩板内张开型裂纹非匀速扩展的瞬态分析问题．在现有断裂动力学问题文献中，一般采用拉氏变换与积分变换方法，得到的回路积分最后还需借助于数值方法求解．作者在断裂动力学基本方程中引入一个非线性阻尼项来考虑能量损耗，采用小参数摄动，得到零级近似动力学方程组，而且自始至终采用解析方法进行计算，获得了问题的零级近似解析解．如果沿裂纹长度上有剪应力作用，采用本文方法也可获得其零级近似解析解．本文方法将有利于改善非线性断裂动力学中应用有限单元法求数值解的收敛性．因而本文为解决这类非线性断裂动力学问题提供了一条新的途径．     

埋地塑料排水管发展浅析

本文介绍了埋地硬聚氯乙烯排水管道的特点及现状,并且分析了其今后的发展前景和趋势,供设计施工参考.