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Martínez Mauro Dinolfo María I. Nogueira Soledad Stenglein Sebastián A. 《植物病害和植物保护杂志》2021,128(5):1377-1381
Journal of Plant Diseases and Protection - The objective of the present work was to evaluate the pathogenicity of Fusarium tricinctum in barley and to determine the potential effects on grain... 相似文献
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Characterization of a Fusarium poae world-wide collection by using molecular markers 总被引:1,自引:0,他引:1
María I. Dinolfo Eliana Castañares Sebastián A. Stenglein 《European journal of plant pathology / European Foundation for Plant Pathology》2014,140(1):119-132
Fusarium poae has been considered as a minor species among those that cause Fusarium Head Blight (FHB) disease but in recent years several researchers have documented a high frequency of occurrence of this species. In this study, a total of 173 F. poae isolates from Argentina, Belgium, Canada, England, Finland, France, Germany, Hungary, Italy, Luxembourg, Poland, Switzerland and Uruguay were evaluated by using inter simple sequence repeats (ISSR) and amplified fragment length polymorphism (AFLP) to evaluate genetic variability within F. poae and to amplify MAT idiomorphs as a possible mechanism that could explain part of the variability found in this species. The molecular analysis obtained from both molecular markers showed a high intraspecific variability. However, a partial clustering between F. poae isolates and their geographic origin was obtained by ISSR markers while AFLP showed isolates from different geographic locations distributed throughout the dendrogram. Moreover, ISSR grouped all the F. poae isolates into a different cluster from the F. langsethiae and F. sporotrichioides isolates used as outgroups compared with the dendrogram obtained using AFLP markers. Analysis of molecular variance (AMOVA) indicated a high genetic variability in the F. poae collection, with most of the genetic variability resulting from differences within, rather than between, American and European populations by using both molecular markers. Regarding MAT idiomorphs, for most F. poae isolates both MAT-1 and MAT-2 were present from each isolate. 相似文献
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María I. Dinolfo Sebastian A. Stenglein María V. Moreno Paul Nicholson Philip Jennings Graciela L. Salerno 《European journal of plant pathology / European Foundation for Plant Pathology》2010,127(4):483-491
Fusarium poae is one of the Fusarium species isolated from cereal grains infected by Fusarium head blight (FHB), and in recent years it has been identified as
a major FHB component. In this study, 97 F. poae isolates from Argentina (n = 62) and England (n = 35) were analysed by inter-simple sequence repeats (ISSR) to examine the genetic diversity and to determine whether intraspecific
variation could be correlated with geographic and/or host origin. The molecular analysis showed high intraspecific variability
within F. poae isolates, but did not reveal a clear relationship between variability and the host/geographic origin. Fusarium poae isolates from the same geographic region or host appeared in different subclusters. Conversely, isolates with the same haplotype
were also collected from different geographic regions. However, we did observe subclusters consisting of isolates from Argentina
only or from England only. Furthermore, a single seed sample was found to host different haplotypes. Analysis of molecular
variance (AMOVA) indicated a high genetic variability in F. poae, with most of the genetic variability explained by differences within, rather than between Argentinean and English populations.
This is the first report on genetic diversity of F. poae using ISSR markers. Moreover, ISSR fingerprinting generates highly polymorphic markers for F. poae and proved to be a useful and reliable assay for genetic variability studies. 相似文献
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Species composition and genetic structure of Fusarium graminearum species complex populations affecting the main barley growing regions of South America 下载免费PDF全文
E. Castañares M. I. Dinolfo E. M. Del Ponte D. Pan S. A. Stenglein 《Plant pathology》2016,65(6):930-939
Members of the Fusarium graminearum species complex (FGSC), such as F. graminearum and F. asiaticum, are the main cause of fusarium head blight (FHB) of wheat and barley worldwide. In this study, 117 FGSC isolates obtained from commercial barley grain produced in Argentina (n = 43 isolates), Brazil (n = 35), and Uruguay (n = 39) were identified to species and trichothecene genotypes, and analysed using amplified fragment length polymorphism (AFLP) and sequence‐related amplified polymorphism (SRAP) markers. In addition, reductase (RED) and trichothecene 3‐O‐acetyltransferase (Tri101) were sequenced for a subset of 24 isolates. The majority of the isolates (n = 103) were identified as F. graminearum, which was the only species found in Argentina. In Uruguay, only one F. cortaderiae isolate was found among F. graminearum isolates. In Brazil, F. graminearum also dominated the collection (22/35), followed by F. meridionale (8/35), F. asiaticum (2/35), F. cortaderiae (2/35) and F. austroamericanum (1/35). Species were structured by trichothecene genotype: all F. graminearum were of the 15‐acetyldeoxynivalenol (ADON), F. meridionale, F. asiaticum and F. cortaderiae were of the nivalenol (NIV), and F. austroamericanum was of the 3‐ADON genotype. Both AFLP and SRAP data showed high levels of genetic variability, which was higher within than among countries. Isolates were not structured by country of origin. SRAP analysis grouped F. graminearum in a separate cluster from the other species within the complex. However, AFLP analysis failed to resolve the species into distinct clades with partial clustering of F. meridionale, F. austroamericanum, F. asiaticum and F. graminearum isolates. 相似文献
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