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Effects of ascorbic acid 2‐glucoside and alpha‐tocopherol on the characteristics of equine spermatozoa stored at 5°C 下载免费PDF全文
Breno Fernandes Barreto Sampaio Bruno Gomes Nogueira Maria Inês Lenz Souza Eliane Vianna da Costa e Silva Carmem Estefânia Serra Neto Zúccari 《Animal Science Journal》2018,89(10):1415-1423
The aim of this experiment was to evaluate the effects of adding ascorbic acid 2‐glucoside (AA2G), a water‐soluble antioxidant and stable derivative of ascorbate, to the semen extender and compare it to the addition of vitamin C (Vit. C) and the fat‐soluble antioxidant α‐tocopherol (α‐Toh), both individually and in combination, on the seminal variables of equine sperm submitted to cooling for 72 h. We used two ejaculates from 10 stallions and evaluated them for motility, membrane integrity, chromatin fragmentation, mitochondrial activity and lipid peroxidation. In the analysis of lipid peroxidation, the control group showed 2506.2 ± 796.4 ng malondialdehyde/108 sperm, which was higher (P < 0.05) than the groups treated with antioxidants. The average value of motility in the AA2G group was 68.4 ± 18.1%, which was higher (P < 0.05) than that observed in the control group (62.1 ± 16.2%). The variables membrane integrity, chromatin fragmentation and mitochondrial activity did not show significant difference (P > 0.05) between treatments. It was concluded that the antioxidants protected sperm cells from lipid peroxidation and that AA2G was effective during the cooling process of equine semen at 5°C for72 h, providing increased levels of total motility. 相似文献
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Macedo GG Zúccari CE de Abreu UG Negrão JA da Costa e Silva EV 《Tropical animal health and production》2011,43(6):1175-1182
This study investigated the effect of human–animal interaction (HAI) and the stress response on the quality of embryo production
in superovulated Nelore (Bos indicus) cattle, under tropical conditions. Thirty-two females underwent a superovulation protocol for 5 days. Cortisol concentrations
were determined in blood plasma collected on days 0, 4, and 5. Artificial insemination was performed on days 4 and 5, and
nonsurgical embryo flushing on day 11. Embryo production and viability were determined. Human stimulation, animal behaviors,
accidents, and handling time were recorded to assess HAI. Cattle age was negatively correlated with accidents, frequency of
aversive behaviors, and negative stimuli by stockperson during transit through corral compartments to receive superovulation
treatments. The factor analysis revealed two distinct groups. The first group was called stressed and had higher cortisol
concentration than the nonstressed group, 16.0 ± 2.1 and 12.5 ± 1.0 ng/mL, respectively. Comparisons between these groups
showed that the frequency of voice emissions by the stockperson and the number of accidents were higher in the stressed group,
and also, the mean handling time was longer in the stressed group than for the nonstressed. As a result, viability rate of
the embryos was 19% lower in the stressed group (P < 0.05). This indicates that intensive negative HAI is likely related to stress, which affects embryo production in a superovulation
program. 相似文献
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