Analysis of Application of Information in Mid - mini Enterprises on Condition of Mass- economy

The group of Mid - mini enterprises under mass - economy is the intermediate organization built up by specialized division of work and cooperation. This kind of organization is flexible in operation with high efficiency, but difficult in development of new technology. On the basis of analyzing the character and deficiency of the mid - mini enterprises, in this paper, through providing a framework of information service platform for the mid - mini enterprises to enhance their creative ability and complex competition. The construction and operational strategy of such platform are investigated. Finally a successful case study is given.     

Model of Information Integrating in CIMS Based on the Object-oriented Method

Information integrating is the most important key to CIMS,which is the base of the function integrating and all others.Because of so many kinds,so complicated structure,so many different sources and uses that the information in CIMS has,it is necessary to build a popular Information Integrating Model. The model of information integrating in the thesis bases on the enterprise model,which is presented by ISO(International Organization for Standardization).According to the object-oriented method,the process of modeling begins with classifying the information,and then describes the definition of the attributes and the structural relation of those objects. By means of the idea of Encapsulation and Inheritance,the destination of information integrating can be arrive and the model of information integrating can be set up. The model which is based on the share information is helpful to finish the function integrating and the whole CIMS' construction. Moreover,the system which is developed following such program can be reused for many times and the developing circle of CIMS can be apparently shortened,too.     

The research in processing fMRI data using temporal-spatial model based on CCA

In the analysis of cognition of human brain,the fMRI is often used.Corelation is a common method in fMRI data detecting,but its ability should be improved.Based on the Canonical Correlation Analysis(CCA),the temporal correlation and spatial correlation are used together to detect the activated area in the fMRI data.By the application of the method in simulation data and fMRI data,it shows that this method is effective in the detecting.     

Production and characterization of monoclonal antibodies reactive with the chicken interleukin-15 receptor alpha chain

We recently cloned the genes encoding chicken IL-15 and IL-15 receptor (R) alpha proteins. In this study, 12 monoclonal antibodies (mAbs) against recombinant chicken IL-15Ralpha were produced and characterized. By enzyme-linked immunosorbent assay (ELISA), all mAbs showed binding specificity for IL-15Ralpha, but not IL-2 or interferon-gamma, and identified a 25.0kDa protein by immunoblot analysis. Flow cytometric analysis revealed negligible expression of IL-15Ralpha on non-activated lymphocytes from the spleen, thymus or bursa, low but detectable expression on macrophages and high expression on concanavalin A-activated spleen lymphoblasts. Established chicken T cell (RP13) and macrophage (HD11) cell lines expressed substantially higher levels of IL-15Ralpha compared with a B cell line (RP9). Two mAbs inhibited IL-15 dependent proliferation of T cells suggesting that the tertiary structure of the protein domain of native IL-15Ralpha that binds to IL-15 is preserved in the recombinant receptor molecule. These mAbs will be useful reagents for further in vitro and in vivo studies of the biological functions of chicken IL-15 and its receptor.     

Flow cytometric assessment of ethylene glycol monoethyl ether on spermatogenesis in rats

The effects of ethylene glycol monoethyl ether (EGEE) on testicular cell populations in rats were investigated by a flow cytometric method. Rats were administered by gavage with EGEE at the various doses of 0 (saline alone), 100, 200, 400, and 800 mg/kg body weight/day for 4 weeks. The treatment of EGEE caused decreases in the weight of testis and epididymis and in the number of testicular cells. Histopathologically, exfoliation of germ cells into the tubular lumen was observed at the doses of above 200 mg/kg. The treatment of EGEE at the dose of 400 mg/kg caused moderate testicular degeneration. A significant depletion of haploid cells and a disproportionate ratio of diploid and tetraploid cells were observed as determined by flow cytometric analysis. These results indicate that the toxic effect of EGEE on the male reproductive system may be strongly associated with the disproportion of testicular germ cells.     

Application of the rpoS gene for the detection of Vibrio anguillarum in flounder and prawn by polymerase chain reaction

Vibrio anguillarum , an opportunistic fish pathogen, is the main species responsible for vibriosis, a disease that affects feral and farmed fish and shellfish, and causes considerable economic losses in marine aquaculture. In this study, we used polymerase chain reaction (PCR) to detect V. anguillarum . PCR specificity was evaluated by amplifying the rpoS gene, a general stress regulator, in six strains of V. anguillarum and 36 other bacterial species. PCR amplified a species-specific fragment (689 bp) from V. anguillarum . Furthermore, the PCR assay was sensitive enough to detect rpoS expression from 3 pg of genomic DNA , or from six colony-forming units (CFU) mL⁻¹ of cultured V. anguillarum . However, the assay was less sensitive when genomic DNA from the infected flounder and prawn was used (limit of detection, 50 ng and 10 ng g⁻¹ tissue, respectively). These data demonstrate that PCR amplification of the rpoS gene is a sensitive and species-specific method to detect V. anguillarum in practical situations.     

Antibacterial and antifungal activity of pinosylvin, a constituent of pine

The antibacterial and antifungal activities of pinosylvin (3,5-dihydroxy-trans-stilbene), a constituent of pine, were studied and compared with those of resveratrol (3,5,4'-trihydroxy-trans-stilbene). Pinosylvin exhibited more potent growth inhibitory activity against Candida albicans and Saccharomyces cerevisiae.