Effect of bFGF on glucose regulated protein 78 expression and apoptosis in human ovarian cancer CAOV3 cells

AIM: To evaluate the effect of bFGF on the apoptosis and the expression of glucose regulated protein 78 (GRP78) in ovarian cancer CAOV3 cells.METHODS: Starvation induced cell apoptosis was conducted. After treatment with bFGF, the cell cycle and apoptosis were determined by FACS analysis and Annexin V/PI staining, respectively. The expression of protein kinase B (PKB) and GRP78 were detected by Western blotting and RT-PCR. RESULTS: Compared to starvation group, the cells treated with bFGF were still viable and increased activation of PKB and high expression of GRP78 were observed, which were prevented by PKB inhibitor wortmannin effectively (P<0.01). CONCLUSION: bFGF plays a critical role in anti-apoptosis and proliferation in human ovarian cancer through PKB signal transduction pathway, partly by upregulating the expression of GRP78.     

《中国草地学报》2006-2008年载文及作者统计分析

通过对2006-2008年出版的《中国草地学报》载文及作者情况进行统计分析，研究其栽文和作者一些规律和特点。     

Study on Site-specific Nutrient Management in Cotton Field

The study on the characteristics of spatial variability of soil nutrients and fertilizer recommendations in cotton field under certain conditions of agricultural management was conducted with GIS and systematic approach for soil nutrient constrains. The results showed that of the spatial variability of soil nutrient was greatly related to the management condition of previous crops. Grid sampling and variable rate application technology (VRAT) were the tools that would hopefully increase fertilizer efficiency. The fertilizers were applied where they were needed and at proper rate. Balance fertilization demonstration showed that fertilizer recommendations according to the available nutrient level in soil could decrease fertilizer cost with 657.4 yuan / ha and increase seed cotton yield by 19.8%. A net profit of the balanced fertilization was 5314.9 yuan / ha higher than that of local fertilization practice.     

Association of polymorphism of Fc receptor γ chain gene at position-29 in promoter with the susceptibility to systemic lupus erythematosus in southern Chinese

AIM:To detect the association between the polymorphism of Fc receptor γ chain gene at position-29 in promoter and systemic lupus erythematosus(SLE).METHODS:The genotypes at position -29 in promoter of Fc receptor γ chain gene were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in 180 patients with SLE and 140 ethnically matched controls in southern China.RESULTS:The frequencies of TT genotype(33.3%) and T allele (54.4%) at position -29 in patients with SLE were significantly higher than those in controls (17.2% and 42.9%, respectively), whereas, the frequencies of GG genotype (24.4%) and G allele (45.6%) in patients with SLE were remarkably lower than those in controls (31.4% and 57.1%, respectively) (P＜0.05). The TT genotype and T allele at position -29 were not associated with lupus nephritis in SLE patients (P＞0.05).CONCLUSION:Our results indicate that the T allele at position -29 in promoter of Fc receptor gene probably contributes to the susceptibility to SLE, but does not play a role in the occurrence of lupus nephritis.     

铅胁迫对金丝草生长及生理生化的影响

采用土培方法,探究不同梯度铅胁迫(0、1000、2000和3000 mg·kg^-1)对金丝草生长形态、体内抗氧化系统和渗透调节物质的影响。结果表明:低浓度(1000 mg·kg^-1)处理会诱导金丝草叶片过氧化物酶(POD)活性、可溶性蛋白(SP)含量和根系抗超氧阴离子自由基活力(ASAFR)、可溶性糖(SS)及脯氨酸(Pro)含量增加,使得金丝草植株总抗氧化能力(T-AOC)处于较高水平,促进了金丝草株高、叶长和生物量的增加。随胁迫浓度增加,高浓度(2000~3000 mg·kg^-1)处理下,金丝草叶片和根系丙二醛(MDA)含量迅速增加,株高、叶长、叶面积和生物量下降,生长受到抑制。但金丝草通过增强叶片和根系POD、过氧化氢酶(CAT)活性来抵御过氧化作用,提高可溶性蛋白和可溶性糖含量维持细胞正常运作,增加根系生物量占比来加强根系发育,一定程度适应了高浓度铅胁迫。综上表明,金丝草主要通过叶片和根系不同抗氧化酶差异化响应、提高渗透调节物质含量,提升金丝草植株总抗氧化能力等途径来提高Pb耐性,对Pb污染矿区植物修复有较大潜力。     

浙江南部近海小黄鱼肌肉脂肪酸组成及食源指示分析

小黄鱼（Larimichthys polyactis）是我国近海重要的经济和优势鱼种,在食物网结构中起承上启下的关键作用。利用气相色谱质谱联用仪测定了浙江南部近海小黄鱼的肌肉脂肪酸含量并分析其组成变化特点,基于特征脂肪酸的食源指示功能开展小黄鱼的食性分析。结果表明：小黄鱼肌肉中共检测出30种脂肪酸,主要脂肪酸包括C16∶0、C17∶0、C18∶1n9c、C18∶3n3、C16∶1n7、C20∶1、C20∶4n6、C20∶5n3（EPA）和C22∶6n3（DHA）等,其中C16∶0含量最高（23.12%）,其次为C18∶1n9c（16.67%）、C16∶1n7（11.45%）和C22∶6n3（11.40%）;肌肉中不饱和脂肪酸（UFA）平均含量达到60.20%,显著高于饱和脂肪酸（SFA）的含量（39.80%）,DHA和EPA在机体中含量占比达15.54%,体现出小黄鱼肌肉具有较高营养价值;双因素方差分析显示饱和脂肪酸含量在体长组间存在显著差异,含量随着体长的增大而减小,而单不饱和脂肪酸（MUFA）含量存在显著的季节性差异;绝大多数特征脂肪酸在各季节的体长组间无显著差异,而120~139 mm体长组和>180 mm体长组的C16∶1n7、C20∶1和C18∶1n9c在冬季的含量显著小于其他季节;基于脂肪酸食源指示功能,小黄鱼属于浮游、底栖与游泳动物混合食性的鱼类,初始碳源包括浮游硅藻类、甲藻类和底栖生物等。本研究利用了特征脂肪酸稳定指示食物来源的特点,进一步补充和验证文献资料中小黄鱼的胃含物分析结果,为小黄鱼的摄食生态学研究提供重要参考。     

蜡梅花离体摊放过程中香气感官评价和挥发性物质分析

蜡梅花是重要的天然花香原料。采用顶空—固相微萃取和气相色谱—质谱联用技术(HS–SPME–GC–MS)结合感官审评,分析了采摘后蜡梅花摊放过程中挥发性物质的变化规律。结果表明,离体蜡梅花在摊放过程中开放状态变化不明显,花枝经清水培养,花苞到全开状态历经32h左右。全开状态蜡梅花的挥发性物质种类随摊放时间的延长呈先增加后降低的趋势,摊放12h最多,此时出现了酮类、醛类和酚类;大部分挥发性物质相对含量在摊放20 h后降低。主要挥发性物质乙酸苄酯在摊放12 h达峰值,较0 h增加35.04%;4–乙基苄醇和α–罗勒烯呈现先增加后降低的趋势;α–罗勒烯在摊放4 h后急剧增加,20 h达最大值;芳樟醇、别罗勒烯和2,6–二甲基–2,4,6–辛三烯在摊放24 h内呈降低趋势,分别较0 h下降了18.32%、78.92%和41.19%。结合感官审评与GC–MS分析结果可知,全开状态的蜡梅花采摘后离体摊放20 h内能较好地保持花香的鲜灵度和浓郁度,且在12～20 h之间挥发性物质种类丰富,具花果香的挥发性物质相对含量较高。研究结果可为蜡梅花作为天然花香原料的更有效利用提供理论参考。     

饲料中维生素A对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢酶活性及基因表达的影响

为研究饲料中维生素A(VA)对青鱼幼鱼生长、血清生化指标和肝脏糖脂代谢相关酶活性及基因表达的影响,实验选取360尾初始体质量为(6.10±0.10) g的青鱼幼鱼,随机分配至3个实验组中,每个实验组设置3个平行。采用单因素实验设计,以无维酪蛋白和明胶为蛋白源、菜籽油为脂肪源、糊精为糖源,同时添加矿物质混合物和维生素混合物(无VA添加)配制成3组实验饲料,分别以饲料1 (Diet1)、饲料2 (Diet2)和饲料3 (Diet3)表示。在饲料1、饲料2和饲料3中分别添加0、2 200和20 000 IU/kg VA醋酸酯(500 000IU/g),经高效液相色谱法(Agilent-1100, Agilent,美国)检测后实验饲料中VA的实际含量分别为178.2、2 058.9和18 436.2 IU/kg,养殖周期为8周。结果显示:饲料中VA缺乏会显著降低青鱼幼鱼的增重率(WGR)和特定生长率(SGR);VA缺乏会显著降低血清血糖(GLU)、甘油三酯(TG)和低密度脂蛋白(LDL)浓度,增加总胆固醇(TCH)浓度。饲料中添加2 058.9IU/kg VA能显著提高肝脏己糖激酶(HK)、磷...     

杂交晚稻机插不同播量条件下育插秧试验研究

以杂交晚稻“江四优992”为供试材料,进行了不同播量软盘育秧机插与常规水育秧手插的对比试验。结果表明：机插软盘育秧随播种量增加,漏插率降低,以90～100g芽谷／盘为最佳播量;机插秧虽然前期分蘖稍慢,但后期具有分蘖稳健、无效分蘖少、成穗率高的特点;机插稻的全生育期比手插稻延长2～3d;与手插稻相比,机插稻的穗粒结构合理,穗粒数多,产量高,平均增产10．2％。