全文获取类型
收费全文 | 1929篇 |
免费 | 129篇 |
国内免费 | 2篇 |
专业分类
林业 | 56篇 |
农学 | 32篇 |
基础科学 | 15篇 |
162篇 | |
综合类 | 550篇 |
农作物 | 56篇 |
水产渔业 | 154篇 |
畜牧兽医 | 933篇 |
园艺 | 38篇 |
植物保护 | 64篇 |
出版年
2021年 | 17篇 |
2020年 | 24篇 |
2019年 | 28篇 |
2018年 | 19篇 |
2017年 | 24篇 |
2016年 | 33篇 |
2015年 | 26篇 |
2014年 | 38篇 |
2013年 | 71篇 |
2012年 | 53篇 |
2011年 | 79篇 |
2010年 | 34篇 |
2009年 | 53篇 |
2008年 | 77篇 |
2007年 | 82篇 |
2006年 | 78篇 |
2005年 | 79篇 |
2004年 | 66篇 |
2003年 | 52篇 |
2002年 | 65篇 |
2001年 | 36篇 |
2000年 | 46篇 |
1999年 | 35篇 |
1998年 | 18篇 |
1996年 | 16篇 |
1995年 | 19篇 |
1992年 | 25篇 |
1991年 | 27篇 |
1990年 | 21篇 |
1989年 | 20篇 |
1988年 | 15篇 |
1987年 | 42篇 |
1986年 | 59篇 |
1985年 | 53篇 |
1984年 | 47篇 |
1983年 | 46篇 |
1982年 | 37篇 |
1981年 | 34篇 |
1980年 | 35篇 |
1979年 | 37篇 |
1978年 | 33篇 |
1977年 | 27篇 |
1976年 | 26篇 |
1975年 | 28篇 |
1974年 | 31篇 |
1973年 | 21篇 |
1972年 | 17篇 |
1971年 | 16篇 |
1970年 | 16篇 |
1969年 | 17篇 |
排序方式: 共有2060条查询结果,搜索用时 15 毫秒
1.
Forecasting the Genetic Impacts of Net Pen Failures on Gulf of Mexico Cobia Populations Using Individual‐based Model Simulations 下载免费PDF全文
Tanya L. Darden John D. Robinson Allan E. Strand Michael R. Denson 《Journal of the World Aquaculture Society》2017,48(1):20-34
Offshore net pen fish farming provides a cost‐efficient means for production of marine finfish, and there is great interest in development of net pen operations in domestic waters. However, there are concerns over the possible genetic and ecological impacts that escaped fish may have on wild populations. We used individual‐based simulations, with parameter values informed by life history and genetic data, to investigate the short‐term (50 yr) impacts of net pen failures on the genetic composition of cobia, Rachycentron canadum, stocks in the Gulf of Mexico. Higher net pen failure rates resulted in greater genetic impacts on the wild population. Additionally, the use of more genetically differentiated source populations led to larger influxes of non‐native alleles and greater temporal genetic change in the population as a result of net pen failure. Our results highlight the importance of considering the appropriate source population for broodstock collection in net pen aquaculture systems and help to provide a general set of best management practices for broodstock selection and maintenance in net pen aquaculture operations. A thorough understanding of the genetic diversity, stock structure, and population demography of target species is important to determine the impact escapees can have on wild populations. 相似文献
2.
Responses of Australian sea lions,Neophoca cinerea,to anthropogenic activities in the Perth metropolitan area,Western Australia 下载免费PDF全文
- Tourist‐based activities, partly due to their rapid increase, have raised concerns regarding the impacts of anthropogenic activity on marine fauna. Documented effects on pinnipeds in proximity to humans include changes in behaviour, site use and potentially higher aggression levels towards people. Effects vary considerably between populations and sites, thus requiring separate assessment of human impacts on activity and energy budgets.
- Responses of the endangered Australian sea lion, Neophoca cinerea, to human visitation were recorded from November 2013 through April 2014. Exposure levels and response types to anthropogenic activities were assessed at two easily accessible locations with different management schemes, Seal (landing prohibited) and Carnac (landing permitted) islands, Western Australia. Exposure levels were measured as both stimulus type (i.e. ‘People’, ‘Paddlers’, ‘Small’, ‘Medium’, and ‘Large vessels’, ‘Tour vessels’, and ‘Jet skies’), and people (‘Direct’, ‘Attract’, ‘Interact’, ‘View’, ‘Incidental’, ‘Water’, ‘Low‐level’), and vessel activities (‘Interact’, ‘Approach/Follow’, ‘Anchor noise’, ‘Engine noise’, ‘Close to beach’, ‘Moderate/Fast travel’, ‘Slow travel’, ‘Transit’, ‘Drift/At anchor’, ‘Aircraft noise’).
- Exposure levels varied significantly between the islands in numbers, stimuli type, duration and minimum approach distances. The instantaneous behaviours of ‘Lift head’, ‘Interact’ and ‘Sit’ were the most frequent responses. ‘Aggressive’ and ‘Retreat’ responses, the highest disturbance levels measured, occurred on Carnac approximately once per day, but rarely on Seal Island. ‘Aggressive’ behaviour towards ‘People’ was observed only on Carnac Island and elicited only by ‘People’. ‘People’, ‘Tour vessels’, and scenic ‘Aircrafts’ on both islands as well as ‘Jet skis’ on Carnac Island had the highest probability of triggering responses. Owing to their relatively high visitation at Seal Island, ‘Paddle powered vessels’, followed by ‘Tour vessels’ elicited the highest number of responses, compared with ‘People’, ‘Small’, and ‘Medium vessels’ at Carnac Island. The majority of responses occurred when any stimulus type was at short‐range (≤10 m), and ‘People’ ‘Viewing’ N. cinerea elicited most. Vessels triggered more responses at larger ranges than ‘People’.
- To limit close‐range access to N. cinerea, one possibility is to close the beach at Carnac Island to human visitation and increase the minimum approach distance by vessels and ‘People’ by installing marker buoys at least 15 m from the shore.
3.
High fluid shear strain causes injury in silver shark: Preliminary implications for Mekong hydropower turbine design 下载免费PDF全文
L. J. Baumgartner G. Thorncraft O. Phonekhampheng C. Boys A. Navarro W. Robinson R. Brown Z. D. Deng 《Fisheries Management and Ecology》2017,24(3):193-198
The Lower Mekong Basin is facing unprecedented threats to fish diversity from hydropower development. There is increasing pressure on developers and construction authorities to design solutions to improve fish survival through turbines, thus protecting the resources in regions being developed for hydropower. A hydraulic characteristic of hydropower turbines with known fisheries impacts is fluid shear stress. Elevated shear stress occurs where rapidly flowing water passes near spillways, screens and within turbine draft tubes. Shear stress can have adverse impacts on fish, but no work has assessed whether this holds true for Mekong River species. A flume was used to determine critical tolerances of silver shark, Balantiocheilos melanopterus (Bleeker), to shear stress rates at a high‐velocity jet which simulated a hydropower turbine. Fish were assessed for injury or mortality following exposure. Results were compared against a no‐shear control. Injury and mortality were greater at higher shear stress exposures. Injuries occurred at all shear exposure levels with mortality at shear levels higher than 600/s. This approach should help design future hydropower turbines if data on other species demonstrate similar results. If the likelihood of adverse impact is high, then shear stress will need to be considered in the design of future hydropower facilities. 相似文献
4.
5.
6.
Exercise Induced Pulmonary Hemorrhage in Horses: American College of Veterinary Internal Medicine Consensus Statement 下载免费PDF全文
7.
The risk of germination loss during storage in two‐row malting barley can be reduced by identifying grain lots that have undergone incipient germination (IG) during harvest. A method based on starch viscosity that utilizes a Rapid Visco Analyzer (RVA) is currently available for IG analysis. A new potential method, based on the measurement of ethanol emission (EE) from whole barley, may be more efficient and less expensive than the RVA procedure. Three storage experiments were performed: experiments 1 and 3 at 25°C and 80% rh, and experiment 2 in unheated, uninsulated buildings. Decline in germination energy (GE) varied from <1% to about 80% during storage. In experiments 1 and 3, R2 for GE loss (expressed as [weeks to 5% loss of GE]0.2) versus EE was 0.76 in both cases, whereas R2 for GE loss versus RVA viscosity was 0.64 and 0.68, respectively. In experiment 2, the greatest loss of GE was associated with high temperatures and relative humidity in July and August. EE performed as well as RVA in the prediction of germination loss in all three trials. Although further evaluation of the EE method is required, it appears to be a promising alternative to the RVA technique. 相似文献
8.
Seedlings of Leptospermum scoparium J.R. et G. Forst (manuka) originating from seed from a low altitude coastal site (Auckland) and from a high altitude inland site (Desert Road) were grown for 96 days in four controlled environments to compare the relationship between growth temperature and frost hardening. Day/night temperature treatments were 12/6, 12/3, 12/0 and 12/-3 degrees C. Frost hardiness was determined at 14-day intervals by exposing whole seedlings to temperatures ranging from -2 to -8 degrees C. Frost damage differed significantly between the two populations: Desert Road seedlings were less affected than Auckland seedlings. At all growth temperatures, the time courses of frost hardiness of both populations followed curvilinear relationships reaching a maximum hardiness at about Day 50, after which the seedlings spontaneously dehardened. The rate of frost hardening increased linearly with decreasing temperature from 6 to 0 degrees C, but thereafter, no further increase occurred with decreasing temperature to -3 degrees C. The frost hardening process was more sensitive to temperature in the Desert Road seedlings than in the Auckland seedlings, and this difference may account for the intraspecific variation in frost hardening capacity of this species. Comparisons with Pinus radiata D. Don and Lolium perenne L. indicated that interspecific variation in frost hardening capacity can also be accounted for by differences in the sensitivity of the hardening process to temperature. 相似文献
9.
Periderm formation was studied in bark samples collected from the roots of western larch (Larix occidentalis) and Douglas‐fir (Pseudotsuga menziesii) trees infected with Armillaria ostoyae. Necrophylactic periderms were formed in advance of infection and successfully restricted continued fungal spread in 68 and 45% of the samples collected from 10‐ and 27‐year‐old western larch, respectively. However, all periderms formed in 11‐ and 25‐year‐old Douglas‐fir had been breached by the advancing fungus. In both species, necrophylactic periderms were commonly breached at the junction of the periderm with the vascular cambium. In western larch, stone phellem often comprised the external phellem layer of necrophylactic periderms with multiple bands of phellem. In 27‐year‐old western larch, infection was often confined to discrete lesions bounded by multiple periderms with multiple bands of phellem. In both tree species, phellem production was greater in response to infection than in response to abiotic wounding. 相似文献
10.
Robinson RM Sturrock RN Davidson JJ Ekramoddoullah AK Morrison DJ 《Tree physiology》2000,20(8):493-502
Protein was extracted from root bark of 11- and 25-year-old interior Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) trees that were naturally infected with Armillaria ostoyae (Romagnesi) Herink. The proteins were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Root bark tissue adjacent to infected areas had a significantly higher protein concentration than healthy tissue (P < 0.05), whereas the protein concentration of infected tissue was consistently lower (P < 0.05) than that of healthy tissue. The SDS-PAGE profiles of healthy, infected, and adjacent-to-infected root bark tissues revealed significant differences in concentrations of a 29.3-kDa protein. The N-terminal amino acid sequence of the 29.3-kDa protein displayed significant homology (P = 0.013) to a basic endochitinase. Use of a polyclonal antibody raised against the 29.3-kDa putative endochitinase-like protein (ECP) indicated differences in the quantities of ECP in healthy roots compared with roots infected with A. ostoyae in 11- and 25-year-old interior Douglas-fir trees. The antibody was also used to screen for the presence of the 29.3-kDa protein in roots of 24-year-old coastal Douglas-fir (Pseudotsuga menziesii var. menziesii) trees that were artificially inoculated with and colonized by Phellinus weirii (Murr.) Gilbn. The amount of ECP was elevated in root bark of coastal Douglas-fir in response to P. weirii infection, although in lower quantities relative to those found in the A. ostoyae-interior Douglas-fir pathosystem. The sequence homology of the ECP with a basic chitinase, together with its increased synthesis in response to two fungal pathogens, indicate a possible role for this protein in the defense of Douglas-fir against fungal pathogens. 相似文献