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1.
Silage and total mixed ration hygienic quality on commercial farms: implications for animal production 下载免费PDF全文
T. Cogan R. Hawkey E. Higgie M. R. F. Lee E. Mee D. Parfitt J. Raj S. Roderick N. Walker P. Ward J. M. Wilkinson 《Grass and Forage Science》2017,72(4):601-613
Implications of silage hygienic quality for animal production were investigated on forty‐five dairy farms in South West England. Samples of grass and maize silages and of total mixed rations (TMR) were obtained together with information on silage technology, herd size and animal production. Samples were analysed for mycotoxins, bacteria, yeasts, moulds and chemical composition. Thirteen mycotoxins were assayed, but none were detected in the samples of grass silage. However, mycotoxins were found in 0·9 of all maize and other silage samples, with deoxynivalenol and zearalenone predominating. There was no relationship between total mycotoxin concentration and mean lactation milk yield per cow. Enterobacteria counts tended to be higher in maize silage than in grass silage and higher still in TMR – a cause for concern. There were no relationships between mould counts and mycotoxin concentrations in silages, implying that mycotoxins may have been produced in the field pre‐ensiling. 相似文献
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SCINTIGRAPHIC TRACKING OF MESENCHYMAL STEM CELLS AFTER PORTAL,SYSTEMIC INTRAVENOUS AND SPLENIC ADMINISTRATION IN HEALTHY BEAGLE DOGS 下载免费PDF全文
Mathieu Spriet Geraldine B. Hunt Naomi J. Walker Dori L. Borjesson 《Veterinary radiology & ultrasound》2015,56(3):327-334
Mesenchymal stem cells have been proposed to treat liver disease in the dog. The objective of this study was to compare portal, systemic intravenous and splenic injections for administration of mesenchymal stem cells to target the liver in healthy beagle dogs. Four healthy beagle dogs were included in the study. Each dog received mesenchymal stem cells via all three delivery methods in randomized order, 1 week apart. Ten million fat‐derived allogeneic mesenchymal stem cells labeled with Technetium‐99m (99mTc)‐hexamethyl‐propylene amine oxime(HMPAO) were used for each injection. Right lateral, left lateral, ventral, and dorsal scintigraphic images were obtained with a gamma camera equipped with a low‐energy all‐purpose collimator immediately after injection and 1, 6, and 24 h later. Mesenchymal stem cells distribution was assessed subjectively using all four views. Pulmonary, hepatic, and splenic uptake was quantified from the right lateral view, at each time point. Portal injection resulted in diffuse homogeneous high uptake through the liver, whereas the systemic intravenous injection led to mesenchymal stem cell trapping in the lungs. After splenic injection, mild splenic retention and high homogeneous diffuse hepatic uptake were observed. Systemic injection of mesenchymal stem cells may not be a desirable technique for liver therapy due to pulmonary trapping. Splenic injection represents a good alternative to portal injection. Scintigraphic tracking with 99mTc‐HMPAO is a valuable technique for assessing mesenchymal stem cells distribution and quantification shortly after administration. Data obtained at 24 h should be interpreted cautiously due to suboptimal labeling persistence. 相似文献
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Ján BURKU? Martina KA?MAROVá Janka KUBANDOVá Natália KOKO?OVá Kamila FABIANOVá Du?an FABIAN Juraj KOPPEL ?tefan ?IKO? 《The Journal of reproduction and development》2015,61(4):325-331
We found retardation of preimplantation embryo growth after exposure to maternal restraint stress during the preimplantation period in our previous study. In the present study, we evaluated the impact of preimplantation maternal restraint stress on the distribution of inner cell mass (ICM) and trophectoderm (TE) cells in mouse blastocysts, and its possible effect on physiological development of offspring. We exposed spontaneously ovulating female mice to restraint stress for 30 min three times a day during the preimplantation period, and this treatment caused a significant increase in blood serum corticosterone concentration. Microscopic evaluation of embryos showed that restraint stress significantly decreased cell counts per blastocyst. Comparing the effect of restraint stress on the two blastocyst cell lineages, we found that the reduction in TE cells was more substantial than the reduction in ICM cells, which resulted in an increased ICM/TE ratio in blastocysts isolated
from stressed dams compared with controls. Restraint stress reduced the number of implantation sites in uteri, significantly delayed eye opening in delivered mice, and altered their behavior in terms of two parameters (scratching on the base of an open field test apparatus, time spent in central zone) as well. Moreover, prenatally stressed offspring had significantly lower body weights and in 5-week old females delivered from stressed dams, fat deposits were significantly lower. Our results indicate that exposure to stress during very early pregnancy can have a negative impact on embryonic development with consequences reaching into postnatal life. 相似文献
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Zoledronate is a bisphosphonate frequently used for the treatment of hypercalcaemia of malignancy and tumour-associated bone pain in dogs, however, there is a paucity of information regarding its use in veterinary medicine. The aim of this retrospective study was to report the tolerability of zoledronate in the palliative treatment of cancer-bearing dogs and secondarily to to assess the efficacy of zoledronate for the treatment of hypercalcaemia of malignancy. Thirty-seven dogs (22 with tumour-associated bone pain and 15 with hypercalcaemia of malignancy) that received 114 zoledronate infusions were included. Tolerability was assessed by the absence of post-zoledronate hypocalcaemia or other adverse events as defined by Veterinary Cooperative Oncology Group-Common Terminology Criteria for Adverse Events criteria. Efficacy was assessed by comparison of available ionized calcium levels before and after zoledronate administration in hypercalcaemic dogs. In 79% of zoledronate infusions, no adverse events were reported. The majority of adverse events which occurred in the other 21% of infusions could be attributed to concurrent chemotherapy or the underlying neoplastic disease. There was a small but significant increase in creatinine following treatment with zoledronate, however, none of the dogs developed clinically significant renal disease. In eight hypercalcaemic dogs with available ionized calcium following zoledronate administration, ionized calcium decreased rapidly within 7 days following treatment with zoledronate. Zoledronate is well-tolerated with few recorded adverse events, however, monitoring of serum creatinine is advised. Zoledronate seems to be effective in the treatment of hypercalcaemia of malignancy. 相似文献
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Kyle E. Walker 《Growth and change》2017,48(4):532-551
Recent estimates that central cities are growing faster than their suburbs in the U.S. have captured the attention of both academics and the popular media. Many commentators have used these numbers to claim that internal migration trends in the U.S. have reached a turning point, in which migrants increasingly prefer urban residences to suburban locales. However, these assertions often rely on problematic definitions of city and suburb, and pay too little attention to demographic variations among migrants. This paper examines whether recent internal migrants in the U.S. are choosing closer‐in destinations, drawing from microdata samples from the American Community Survey since 2005. During this period, there is an overall trend of migrants to the largest metropolitan areas in the U.S. choosing to migrate closer to the metropolitan core. However, this trend varies significantly among major demographic groups; whereas younger, single, educated, and white‐collar migrants show evidence of a return to the core, migrants who are blue‐collar, less‐educated, older, and with families remain more suburban. In turn, this analysis suggests that overall trends of “back to the city” migration are producing considerable divergence in the metropolitan destinations of different demographic groups. 相似文献
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M. ŘÍHA J. KUBEČKA M. PRCHALOVÁ T. MRKVIČKA M. ČECH V. DRAŠTÍK J. FROUZOVÁ E. HOHAUSOVÁ T. JŮZA M. KRATOCHVÍL J. PETERKA M. TUŠER M. VAŠEK 《Fisheries Management and Ecology》2011,18(4):339-347
Abstract Diel changes in littoral fish assemblage were studied in four reservoirs in the Czech Republic (Central Europe). The sampling was performed by beach seining in an unstructured littoral zone. Perch, Perca fluviatilis L., roach, Rutilus rutilus (L.), bream, Abramis brama (L.), carp, Cyprinus carpio L., ruffe, Gymnocephalus cernua (L.), pikeperch, Sander lucioperca (L.), eel, Anguilla anguilla (L.) and A. brama × R. rutilus hybrids exhibited higher densities at night. Only bleak, Alburnus alburnus (L.) exhibited higher densities during the day. The number of species was higher in night hauls, and mean body size of roach and bleak was also higher at night. Diel changes in fish densities resulted in the change in species composition between day and night. The study has implication for the sampling design to assess littoral fish assemblages using beach seine netting and recommends night sampling for a representative assessment or sampling during both diel periods for a robust assessment. 相似文献
8.
Morphology and rooting of shoots developing in response to decapitation and pruning of Caribbean pine 总被引:2,自引:0,他引:2
The effects of decapitation at various levels, combined with heavy pruning of remaining branches, were examined for Caribbean pine. This treatment stimulated the development of shoots with long primary needles at all levels in the crown of trees aged one, four and eight years. Although a significant positive relationship between primary needle length and rooting was demonstrated, the very juvenile appearance of these shoots was not a reliable guide to rooting. There was an over-riding effect on rooting of the ortet age × level in crown × decapitation height treatment. Decapitated trees provided shoots of higher rooting capacity than intact trees for the one and four, but not the eight year old trees. Shoots developing in the lower crown in response to decapitation and heavy pruning of four year old trees displayed a high level of rooting-equivalent to that of shoots from the decapitated and pruned one year old trees. 相似文献
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Christoforos Karanikas Vincent Walker Apostolos Scaltsoyiannes Gilles Comte Cédric Bertrand 《Annals of Forest Science》2010,67(4):412-412
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