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1.
Analysis of genomic mutation and immunohistochemistry of platelet‐derived growth factor receptors in canine vascular tumours 下载免费PDF全文
S. Abou Asa T. Mori K. Maruo A. Khater A. El‐sawak E. Abd el‐Aziz T. Yanai H. Sakai 《Veterinary and comparative oncology》2015,13(3):237-245
We examined whether mutation of the platelet‐derived growth factor receptor protein tyrosine kinase (PDGFR)‐α and PDGFR‐β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans‐ or juxtamembrane regions of PDGFR‐α and PDGFR‐β were analysed with immunohistochemical staining and polymerase chain reaction‐direct sequencing using DNA from paraffin‐embedded neoplastic tissues of 27 hemangiosarcomas (HSAs) and 20 hemangiomas (HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR‐α and almost most of the HA cases were negative for PDGFR‐β. Of the HSA cases, 55.6% were negative for PDGFR‐α and 63% were strongly positive for PDGFR‐β. Among the HA cases, 1 missense mutation was detected in PDGFR‐α exon 18 and 1 in PDGFR‐β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR‐β. Thus, genomic mutation of trans‐ or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA. 相似文献
2.
Yasushi Ohmachi Tomomi Imamura Mizuyo Ikeda Eriko Shishikura Eunjoo Kim Osamu Kurihara Kazuo Sakai 《Journal of toxicologic pathology》2015,28(2):65-71
To evaluate the effectiveness of sodium bicarbonate (SB) in removing uranium and protecting animals from uranium toxicity, we intramuscularly administered 1 mg/kg of uranyl nitrate to 8-wk-old male SD rats, and 20 min after administration of uranyl nitrate, the animals were given a single oral administration of SB at 0.1, 0.3 or 1 g/kg. The SB treatment at a dose of 0.3 g/kg or more raised the pH of the rats’ urine until 4 h after treatment, and it significantly reduced the uranium amounts in the kidneys at 1 day after treatment. In another experiment, rats were intramuscularly administered 1 mg/kg of uranyl nitrate, and 20 min later, the animals were treated with sodium bicarbonate (0.1 or 1 g/kg). The rats were autopsied at 1, 3 and 7 days after uranium treatment. High-dose SB resulted in a significant increase in urinary uranium excretion in the first 24 h and a reduction of uranium deposition in the kidneys and femurs, and it also significantly suppressed uranium-induced renal toxicity, as shown by both histopathology and clinical chemistry at 3 days after uranium treatment. Low-dose SB did not show such marked effects. Our findings demonstrated that the uranium decorporation effect of sodium bicarbonate was observed at the dosage showing urine alkalinization in rats and that decorporation effect of sodium bicarbonate might be beneficial if it is administered immediately after incorporation of soluble uranium. 相似文献
3.
Masaru Sakai Shin‐ichi Suda Taichi Okeda Ryosuke Nomura Izumi Washitani 《水产资源保护:海洋与淡水生态系统》2019,29(5):682-692
- Glossy, broad‐leaved, evergreen (lucidophyllous) forests are found mainly in humid subtropical regions of East Asia and are recognized as a biodiverse biome harbouring numerous endemic species. To date, however, few studies have considered the conservation importance of rivers draining these unique environments. In this study, lotic Odonata were used as indicators to examine factors affecting riparian forest–stream linkages in a lucidophyllous forest in south‐western Japan.
- Lotic odonates of 10 species, including seven endemic species, and their habitats were studied along 30 stream reaches with varying environmental characteristics.
- Odonate species richness was greatest in shadier reaches as well as in heterogeneous locations in larger streams. In contrast, larger streams modified by channel enlargement for flood control had few or no odonate species.
- Protecting larger streams with less human impact and streams in dense riparian forest are the best options for conserving lotic odonates and their habitats in this globally unique forest type.
4.
Chikaya Sakai Atok Subiakto Hani Sitti Nuroniah Naoto Kamata Koji Nakamura 《Journal of Forest Research》2002,7(2):73-80
We developed a vegetative propagation system for mass-producing three dipterocarps species,Shorea selanica Bl.,Shorea leprosula Mig., andShorea platyclados Sloot. This system uses fog evaporative cooling inside a greenhouse to reduce the leaf-to-air vapor pressure deficit (leaf-to-air
VPD) inside the propagator, even under high irradiance conditions. This cooling method has no negative influence on medium
conditions such as overwetting. A plastic tent propagator combined with this cooling method was used for vegetative propagation
experiments. In mass-production experiments, the annual rooting percentages from the cuttings were low in the first 2 years
(1997–1998) due to operational problems of the tent propagator (S. selanica, 48–51%;S. leprosula, 56–59%;S. platyclados: 50–63%). A hard cover propagator improved the rooting percentages in the mass-production experiments in 1999 because it
made operations easier (S. selanica, 70%;S. leprosula, 77%;S. platyclados, 77%). This system, which uses a combination of fog evaporative cooling and a hard plastic propagator, should be useful for
the mass propagation of these dipterocarp planting stocks.
This study was carried out under a joint project of the Ministry of Forestry, Indonesia and Komatsu Ltd. The project was supported
by Research Association for Reforestation of Tropical Forest (RETROF) organized by Japanese Forestry Agency. 相似文献
5.
Tomoya Okunishi Naohiro Takaku Patcharawadee Wattanawikkit Norikazu Sakakibara Shiro Suzuki Fukumi Sakai Toshiaki Umezawa Mikio Shimada 《Journal of Wood Science》2002,48(3):237-241
Lignan production in callus and cell suspension cultures ofDaphne odora is reported for the first time. The cell suspension culture produced pinoresinol, lariciresinol, secoisolariciresinol, matairesinol, and wikstromol. The production of matairesinol in the cell suspension culture was much higher than that inDaphne odora stem tissues.Part of this report was presented at the 51th annual meeting of the Japan Wood Research Society, Tokyo, April 2001 相似文献
6.
Shigeru Yamauchi Yoichi Sakai Yasuo Watanabe Michael Kenya Kubo Hideaki Matsue 《Journal of Wood Science》2007,53(4):324-331
The distributions of boron in Japanese cedar (Cryptomeria japonica D. Don) sapwood blocks treated with aqueous or methanolic boric acid [B(OH)3] solutions were explored through Raman spectroscopy and prompt gamma-ray analysis (PGA). B(OH)3 was the sole boron species observed in Raman spectra of the wood blocks treated with either solution. Plots of weight gain
of the treated wood blocks versus boron concentration in treatment solutions were found to be linear. The results indicated
that the methanolic solution makes it possible to impregnate wood with much larger amounts of boron than the aqueous solution.
PGA confirmed that B(OH)3 was highly enriched near the end grains of the treated wood blocks. Raman measurements suggested that boron content in the
bulk of the wood block is not as large as expected from the weight gain of the treated wood blocks when an ordinary air-drying
method is used. It was concluded that the aqueous solution impregnates the cell walls of wood with boron more easily than
the methanolic solution.
Part of this report was presented at the 55th (Kyoto, March 2005) and 56th (Akita, August 2006) Annual Meetings of the Japan
Wood Research Society. This article follows the previous rapid communication “Analysis of boron in wood treated with boric
acid solutions using Doppler broadening method of prompt gamma-rays.” J Wood Sci (2006) 52:279–281 相似文献
7.
Yasuyuki Tachiki Tetsuhiko Yoshimura Hisashi Hasegawa Tomonori Mita Tetsuro Sakai Futoshi Nakamura 《Journal of Forest Research》2005,10(6):419-427
The objectives of this study were to determine Global Positioning System (GPS) positional errors while moving under the forest
canopy and to clarify the effects of polyline simplification on area and perimeter estimations. We used the Pathfinder Pro
XR and GPSMAP 76S, which are categorized as “high-end mapping” and “general navigation” GPS receivers, respectively. The field
tests were conducted in both natural and plantation forests. The results showed that the Pathfinder Pro XR, which has better
multipath rejection technology, worked well, especially in the plantation forest under unfavorable conditions of higher stand
density. We used analysis of variance to clarify the effects of the receiver type, positioning mode, stand type, and polyline
simplification method on area and perimeter estimations. The receiver type and positioning mode were found to be significant
factors that affected area estimation. The Pathfinder Pro XR estimated the area more accurately than the GPSMAP 76S, and differential
GPS estimated the area more accurately than autonomous GPS. With respect to the perimeter, the receiver type, positioning
mode, and polyline simplification method were found to be significant factors. The results showed that perimeter estimation
was improved by using the velocity filter, and further improved by using the velocity filter and Douglas-Peucker algorithm,
especially when the Pathfinder Pro XR was used. The GPSMAP 76S estimated the perimeter accurately without any filtering because
its default speed filter worked well, even though the GPSMAP 76S is a general navigation GPS receiver. 相似文献
8.
Takuyuki Yoshioka Kazuhiro Aruga Toshio Nitami Hiroshi Kobayashi Hideo Sakai 《Journal of Forest Research》2005,10(2):125-134
Using the method of a life cycle inventory (LCI) analysis, the energy balance and the carbon dioxide (CO2) emission of logging residues from Japanese conventional forestry as alternative energy resources were analyzed over the entire life cycle of the residues. The fuel consumption for forestry machines was measured in field experiments for harvesting and transporting logging residues at forestry operating sites in Japan. In addition, a total audit of energy consumption was undertaken. It involved an assessment of materials, construction, and the repair and maintenance of forestry machines as well as the costs associated with an energy-conversion plant. As a result, the ratio of energy output to input was calculated to be 5.69, indicating that the system examined in this study could be feasible as an energy production system. The CO2 emission per MWhe (e: electricity) of the biomass-fired power generation plant was calculated to be 61.8kgCO2/MWhe, while that of coal-fired power generation plants in Japan is 960kgCO2/MWhe. Therefore, the reduction in the amount of CO2 emission that would result from replacing coal with biomass for power generation by as much as 3.0 million dry-t/year of logging residues in Japan was estimated to be 1.66 million tCO2/year, corresponding to 0.142% of the national CO2 emission. This study provides evidence that Japan could reduce its domestic CO2 emission by using logging residues as alternative energy resources. 相似文献
9.
Shinpei Banno Hidenari Saito Hiroshi Sakai Toshihiko Urushibara Kentaro Ikeda Takeshi Kabe Isao Kemmochi Makoto Fujimura 《Journal of General Plant Pathology》2011,77(5):282-291
Verticillium longisporum and V. dahliae, causal agents of Verticillium wilt, are spreading through the cabbage fields of Gunma Prefecture. Using the V. longisporum-specific intron within the 18S rDNA and differences between ITS 5.8S rDNA sequences in Japanese isolates of V. longisporum and V. dahliae, we developed three quantitative nested real-time (QNRT) PCR assays. The QNRT-PCR quantification of V. longisporum or V. dahliae in cabbage field soil was consistent with the severity of Verticillium wilt disease in those fields. In field trials of resistant
cultivar YR Ranpo grown for three seasons in soil infested with the pathogen, disease severity and pathogen density in the
soil were significantly reduced in a field moderately contaminated by V. dahliae, but only slightly reduced in a highly contaminated field. These results suggest that continuous cultivation of a resistant
cultivar is an effective way to reduce the pathogen population. QNRT-PCR assays provide a powerful analytical tool to evaluate
the soil population dynamics of V. longisporum and V. dahliae for disease management. 相似文献
10.
Jean Fall Gunimala Chakraborty Tomoya Kono Minoru Maeda Yoshihiro Suzuki Toshiaki Itami Masahiro Sakai 《Fisheries Science》2011,77(1):129-134
Vibrio nigripulchritudo is considered one of the major pathogens threatening shrimp aquaculture. In this study, we developed a novel and highly specific
quantitative loop-mediated isothermal amplification (Q-LAMP) assay. A set of four specific primers were designed targeting
the V. nigripulchritudo intergenic spacer region. The reaction time and temperature were optimized for 60 min at 63°C. Quantitative analysis was
then performed by measuring the turbidity of the reaction solution using a real-time turbidimeter, allowing for quantification
of the initial DNA concentration with a sensitivity of 102 copy numbers equivalent to 2.3 colony forming units/ml or 0.3 fg/μl. The LAMP assay was able to specifically detect two representative
strains of V. nigripulchritudo, whereas other Vibrio and non-Vibrio species were not amplified. A standard curve was generated for V. nigripulchritudo by plotting the threshold time (T
t) versus the log of bacterial number. A high correlation coefficient (R
2 = 0.9749) was observed for the Q-LAMP reaction. In conclusion, Q-LAMP assay is a sensitive, rapid, and simple tool that can
be used for the detection and quantification of V. nigripulchritudo in shrimp, thereby facilitating surveillance of vibriosis infection. 相似文献