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1.
Nicholas A Lyons Neal Alexander Katharina DC St?rk Thomas D Dulu Jonathan Rushton Paul EM Fine 《Veterinary research》2015,46(1)
Foot and mouth disease (FMD) is a highly transmissible viral infection of cloven hooved animals associated with severe economic losses when introduced into FMD-free countries. Information on the impact of the disease in FMDV-endemic countries is poorly characterised yet essential for the prioritisation of scarce resources for disease control programmes. A FMD (virus serotype SAT2) outbreak on a large-scale dairy farm in Nakuru County, Kenya provided an opportunity to evaluate the impact of FMD on clinical mastitis and culling rate. A cohort approach followed animals over a 12-month period after the commencement of the outbreak. For culling, all animals were included; for mastitis, those over 18 months of age. FMD was recorded in 400/644 cattle over a 29-day period. During the follow-up period 76 animals were culled or died whilst in the over 18 month old cohort 63 developed clinical mastitis. Hazard ratios (HR) were generated using Cox regression accounting for non-proportional hazards by inclusion of time-varying effects. Univariable analysis showed FMD cases were culled sooner but there was no effect on clinical mastitis. After adjusting for possible confounders and inclusion of time-varying effects there was weak evidence to support an effect of FMD on culling (HR = 1.7, 95% confidence intervals [CI] 0.88-3.1, P = 0.12). For mastitis, there was stronger evidence of an increased rate in the first month after the onset of the outbreak (HR = 2.9, 95%CI 0.97-8.9, P = 0.057).
Electronic supplementary material
The online version of this article (doi:10.1186/s13567-015-0173-4) contains supplementary material, which is available to authorized users. 相似文献2.
An isolate of the fungus Fusarium culmorum constitutively expressing green fluorescent protein was used to investigate the infection process and host response of primary seedling roots and stem base leaf sheaths of soft wheat cv. Genio. Disease progress was assessed macroscopically by visual symptoms, microscopically by confocal laser scanning microscopy (CLSM) and via gene expression analysis of fungal and wheat genes by real‐time quantitative RT‐PCR. In the roots, CLSM investigations revealed an initial intercellular and subsequent intracellular colonization by fungal hyphae. The fungus invaded the rhizodermal layer and cortex but was not seen to colonize the stele. The fungus consistently expressed TRI5 (24, 48 and 96 h post‐inoculation), indicating that trichothecenes were being synthesized throughout this phase of infection and colonization. The expression of the six host defence‐associated genes (Wheatwin 1‐2, PR1, Chitinase, PAL, WIR1 and LOX) increased early in infection and decreased during later stages. In the stem base, CLSM observations revealed the fungus sequentially penetrating though the first, second and third basal leaf sheaths. Expression of TRI5 was initiated early in the infection of each leaf sheath. The expression of the host defence‐associated genes varied over time and across leaf sheaths, and all were also expressed in leaf sheaths which had not yet been in contact with the fungus. Expression of LOX and WIR1 were particularly enhanced in the third leaf sheath. 相似文献
3.
J. E. Nicholson 《Wood Science and Technology》1971,5(1):40-48
Summary A method of rapidly determining the longitudinal growth stress present on the surface of logs and standing trees is described. Application of the technique will enable detailed examination of stress present about the circumference and along the length of trees and logs. In addition it will enable comparisons to be made between trees, as well as monitoring changes occurring in a particular log as the result of treatment to reduce stress. Finally, its use will enable the selection of low stressed trees for genetic studies and propagation trials.The author would like to thank members of the staff of the Division, and in particular Mr. J. Barnacle, for valuable discussion relating to this work. In addition, thanks are due to Mr. K. Murray for participation in the design and construction of the equipment described. 相似文献
4.
Getaneh K. Ayele Azalu A. Gessess Meseret B. Addisie Seifu A. Tilahun Tigist Y. Tebebu Daregot B. Tenessa Eddy J. Langendoen Charles F. Nicholson Tammo S. Steenhuis 《Land Degradation \u0026amp; Development》2016,27(2):270-280
Gully erosion reduces agricultural productivity by destroying valuable land resources, increases sediment concentrations, reduces water quality, and fills up reservoirs. Gully rehabilitation has proven to be challenging especially in the high‐rainfall areas of the Ethiopian Highlands and has therefore had limited success. This paper describes a successful low‐cost gully rehabilitation effort with community participation in the Birr watershed in the Blue Nile basin that begun in early 2013. Initially, farmers were reluctant to participate for religious reasons, but with the aid of local priests and respected elders, community discussions, and a visit to a rehabilitated gully, a consensus was reached to rehabilitate a 0·71‐ha upland gully. The rehabilitation measures consisted of regrading the gully head at a 45° slope, constructing low‐cost check dams from locally available materials, and planting Pennisetum purpureum grass and Sesbania sesban. At the end of the first post‐implementation rainy season, 2,200 tons of soil was conserved by the constructed check dams and newly planted vegetation, compared with soil losses of 680 and 560 tons in two untreated, nearby gullies. In 2014, an additional 3,100 tons of soil was conserved. In 2013, the marginal rate of return (MRR) on the gully rehabilitation investment was 2·6 based on the value of increased forage production alone. When we include trapped soil nutrient values, the rehabilitation MRR was increased to 10. Although these numbers are impressive, the best proof of the success was that farmers on their own initiative rehabilitated an additional five gullies in 2014. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
5.
N. Gosman† Srinivasachary A. Steed E. Chandler M. Thomsett P. Nicholson 《Plant pathology》2010,59(1):147-157
A series of experiments was conducted to determine whether type I resistance (resistance to initial infection) to fusarium head blight (FHB) in wheat could be assessed using fungal species/isolates that do not produce deoxynivalenol (DON), a mycotoxin critical to the spread of Fusarium graminearum in the wheat spike. It was shown that, while the non-toxin-producing species Microdochium nivale and M. majus could infect following spray inoculation of wheat spikes, they were unable to spread within the spike following point inoculation. However, although these species might reveal type I resistance, they are not highly pathogenic towards wheat. A nivalenol (NIV)-producing isolate of F. graminearum caused high levels of disease following spray inoculation, but spread only very slowly within the spike and rarely induced bleaching above the point of inoculation. It is proposed that spray inoculation with an appropriate, aggressive, non-DON-producing FHB pathogen may be used to characterize type I resistance to complement point inoculation with a DON-producing isolate to assess type II resistance (resistance to spread within the spike). 相似文献
6.
7.
8.
Within-field variability of Fusarium head blight pathogens and their associated mycotoxins 总被引:1,自引:0,他引:1
X.-M. Xu D. W. Parry P. Nicholson M. A. Thomsett D. Simpson S. G. Edwards B. M. Cooke F. M. Doohan S. Monaghan A. Moretti G. Tocco G. Mule L. Hornok E. Béki J. Tatnell A. Ritieni 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(1):21-34
Within-field variability in the Fusarium head blight (FHB) and its associated mycotoxins was studied in four European countries.
At each of 14 sites, each FHB pathogen and associated mycotoxins were quantified in 16 quadrat samples at harvest. Overall,
the incidence of quadrat samples with detectable and quantifiable pathogen DNA was significantly lower in the grain than in
the corresponding chaff. Deoxynivalenol (DON) was the most frequently detected toxin in the samples and its accumulation was
most strongly associated with the presence of Fusarium graminearum. Nivalenol (NIV) accumulation was significantly associated only with the presence of F. culmorum. Zearalenone (ZON) accumulation was strongly associated with the presence of all three pathogens (F. graminearum, F. culmorum and F. poae). The levels of both DON and ZON concentrations were positively related to the amount of F. graminearum DNA in the grain or in the chaff. The presence/absence of FHB pathogens within a single quadrat appeared to be independent
of each other. The presence of a particular FHB pathogen and the amount of its DNA, as well as the associated mycotoxin(s),
varied greatly among samples at each site. This study demonstrated the large extent of within-field variability of FHB and
its associated mycotoxins, and the importance of representative sampling in FHB studies. 相似文献
9.
Objective Validation of a stimulation test for determining the steroidogenic capacity of the parrot testis. The major aim was to characterise testosterone secretion after injection of a gonadotropin-releasing hormone agonist (GnRHa), then use the test to investigate seasonal reproduction in the male cockatiel.
Procedure A synthetic GnRHa (buserelin; 8.0 µg of peptide/kg bodyweight) was injected IM into male cockatiels (n = 7) and sulphur-crested cockatoos (n = 3) and serial blood samples collected at 0, 30, 60, 90 and 120 min after administration. Once validated, the technique was subsequently used to examine seasonal changes (23 months) in the testosterone profile of a captive cockatiel population.
Results Injection of buserelin resulted in a significant increase in the testosterone concentration of cockatiel plasma, with maximal concentrations occurring at approximately 60 (1.33 ± 0.08 ng/mL) to 90 min (1.22 ± 0.08 ng/mL) after injection. Although no clear pattern of seasonal variation in testosterone secretion was detected in cockatiel plasma, samples taken 60 and 90 min after administration showed a significant increase in all seasons. Injection of buserelin in the sulphur-crested cockatoo also resulted in increased testosterone secretion, with maximal concentrations obtained after 90 min.
Conclusion Buserelin can be used to obtain a reliable index of the prevailing testosterone capacity of the cockatiel and cockatoo testis. With further studies, this test may be incorporated into clinical assessment of reproductive status. 相似文献
Procedure A synthetic GnRHa (buserelin; 8.0 µg of peptide/kg bodyweight) was injected IM into male cockatiels (n = 7) and sulphur-crested cockatoos (n = 3) and serial blood samples collected at 0, 30, 60, 90 and 120 min after administration. Once validated, the technique was subsequently used to examine seasonal changes (23 months) in the testosterone profile of a captive cockatiel population.
Results Injection of buserelin resulted in a significant increase in the testosterone concentration of cockatiel plasma, with maximal concentrations occurring at approximately 60 (1.33 ± 0.08 ng/mL) to 90 min (1.22 ± 0.08 ng/mL) after injection. Although no clear pattern of seasonal variation in testosterone secretion was detected in cockatiel plasma, samples taken 60 and 90 min after administration showed a significant increase in all seasons. Injection of buserelin in the sulphur-crested cockatoo also resulted in increased testosterone secretion, with maximal concentrations obtained after 90 min.
Conclusion Buserelin can be used to obtain a reliable index of the prevailing testosterone capacity of the cockatiel and cockatoo testis. With further studies, this test may be incorporated into clinical assessment of reproductive status. 相似文献
10.
OLIVER M. CROWE BVSc Cert ES RICHARD J. HEPBURN BVSc Cert EM Diplomate ACVIM SVEND E. KOLD DVM PhD ROGER K. SMITH MA Vet MB PhD DEO Diplomate ECVS 《Veterinary surgery : VS》2010,39(1):107-114
Objective— To report long-term outcome after arthroscopic removal of fragmentation of the extensor process of the distal phalanx in horses.
Study Design— Case series.
Animals— Adult horses (n=13).
Methods— Medical records (2003–2004) of horses that had arthroscopic debridement of fragmentation of the extensor process of the distal phalanx were reviewed. Inclusion criteria included: lameness localized to the foot, fragmentation of the extensor process of the distal phalanx debrided arthroscopically, and a follow-up period of ≥4 years.
Results— Of the 13 horses, lameness was resolved in 11 (85%) initially but distal interphalangeal joint pain recurred in 2 (15%) within 1 year of surgery. Three (23%) other horses were retired because of lameness at other sites during the follow-up period resulting in 46% (6/13) being not lame and in full work after 4 years. Substantial changes were identified at surgery in the contralateral joint of 7 horses, even when fragments were only present unilaterally.
Conclusions— Arthroscopic debridement of fragmentation of the extensor process of the forelimb distal phalanx has a good short-term prognosis for resolution of lameness and return to work but a more guarded prognosis for long-term soundness.
Clinical Relevance— These results allow for more accurate prognostication preoperatively and may support early debridement of fragmentation of the extensor process of the distal phalanx. 相似文献
Study Design— Case series.
Animals— Adult horses (n=13).
Methods— Medical records (2003–2004) of horses that had arthroscopic debridement of fragmentation of the extensor process of the distal phalanx were reviewed. Inclusion criteria included: lameness localized to the foot, fragmentation of the extensor process of the distal phalanx debrided arthroscopically, and a follow-up period of ≥4 years.
Results— Of the 13 horses, lameness was resolved in 11 (85%) initially but distal interphalangeal joint pain recurred in 2 (15%) within 1 year of surgery. Three (23%) other horses were retired because of lameness at other sites during the follow-up period resulting in 46% (6/13) being not lame and in full work after 4 years. Substantial changes were identified at surgery in the contralateral joint of 7 horses, even when fragments were only present unilaterally.
Conclusions— Arthroscopic debridement of fragmentation of the extensor process of the forelimb distal phalanx has a good short-term prognosis for resolution of lameness and return to work but a more guarded prognosis for long-term soundness.
Clinical Relevance— These results allow for more accurate prognostication preoperatively and may support early debridement of fragmentation of the extensor process of the distal phalanx. 相似文献