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排序方式: 共有182条查询结果,搜索用时 15 毫秒
1.
Megumi ITOH Yoshie SAKURAI Yasuhiro NAKAJIMA Satoshi KAWAMOTO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1655-1657
The association between blood calcium levels and electrocardiographic variables was
compared in 137 normal parturient and 36 peripartum recumbent Holstein cows to determine
whether hypocalcemia in peripartum dairy cows can be rapidly diagnosed using
electrocardiograph. Inverse of STc (ST peak interval/SS interval0.5) and blood
ionized calcium or serum calcium concentrations were strongly correlated, and both
correlation coefficients were 0.81 (P<0.001). The 95% prediction
interval indicated that cows with STc >0.385 ± 0.001 sec are very likely to be
hypocalcemic (blood ionized or serum calcium concentrations of <0.9
mmol/l or <7.5 mg/dl, respectively). These findings
indicate that hypocalcemia in parturient cows can be non-invasively estimated using the
STc. 相似文献
2.
Kazuya KUSHIDA Urs GIGER Toshihiko TSUTSUI Megumi INABA Yoshio KONNO Kureha HAYASHI Kana NOGUCHI Akira YABUKI Keijiro MIZUKAMI Moeko KOHYAMA Yasuyuki ENDO Osamu YAMATO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):743-746
Erythrocyte pyruvate kinase (PK) deficiency is an inherited glycolytic erythroenzymopathy
caused by mutations of the PKLR gene. A causative mutation of the feline
PKLR gene was originally identified in Abyssinian and Somali cats in
the U.S.A. In the present study, a TaqMan probe-based real-time PCR genotyping assay was
developed and evaluated for rapid genotyping and large-scale screening for this mutation.
Furthermore, a genotyping survey was carried out in a population of four popular purebred
cats in Japan to determine the current mutant allele frequency. The assay clearly
displayed all genotypes of feline PK deficiency, indicating its suitability for
large-scale survey as well as diagnosis. The survey demonstrated that the mutant allele
frequency in Abyssinian and Somali cats was high enough to warrant measures to control and
prevent the disease. The mutant allele frequency was relatively low in Bengal and American
Shorthair cats; however, the testing should still be carried out to prevent the spread of
the disease. In addition, PK deficiency should always be considered in the differential
diagnosis of anemia in purebred cats in Japan as well as worldwide. 相似文献
3.
As a new use of bamboo, bamboo cubes may be useful as a shot-blast material for surface treatments. However, a suitable processing
technique for bamboo cubes has not been established. In this study, to obtain basic knowledge regarding the processing technology
for bamboo cubes, we installed shearing blades (upper and lower blades) in a universal testing machine to test cross-sectional
shearing of bamboo. The shearing force generated in this shearing was composed of a vertical component (F
v), a forward component (F
f), and a side component (F
s). This shearing force (F
v, F
f, and F
s) and the machining accuracy were investigated under various processing conditions. The shearing force became larger as the
thickness of bamboo increased. In particular, F
v showed a tendency to increase rapidly. Changes in the shear angle of the upper blade had a remarkable influence on F
v. This result suggests that the shearing force could be greatly decreased by adjusting the shear angle. The shearing force
within bamboo of 3mm in thickness was almost unaffected by the blade angle, and the change of shearing force with increasing
clearance was almost indiscernible. It was clearly demonstrated that a large number of bamboo cubes could be made when the
shear angle was large and cross-sectional shearing was performed from the bark side. However, a consequence of a large shear
angle is that burr area increases.
Part of this article was presented at the 53rd Annual Meeting of the Japan Wood Research Society, Fukuoka, March 2003 相似文献
4.
Due to a processing error, first author's name is missing in the HTML version of this article abstract page. The correct authors
are given below:
Jun Mu, Tohru Uehara, Takeshi Furuno
The online version of the original article can be found at 相似文献
5.
Yuko Ota Megumi K. Kimura Tsutomu Hattori Yurika Miyuki Ryota Endo 《Journal of Forest Research》2016,21(2):105-109
Trunk rot, caused by Fomitiporia torreyae, is one of the most economically important sap rot diseases on Cryptomeria japonica, especially on a cutting cultivar ‘Sanbu-sugi’. This disease had been reported only from Chiba and Ibaraki prefectures in Japan; however, a similar trunk rot on Japanese cedars was found recently in Kyoto prefecture. We identified the causal agent of the trunk rot on Japanese cedars in Kyoto prefecture, clarified the genotype of ‘Sanbu-sugi’ (sensu stricto) using microsatellite markers, and then investigated the relatedness of these infected cedars with ‘Sanbu-sugi’. The pathogen was identified as F. torreyae by its DNA sequences and morphological characteristics of the fruiting body. The 25 ramets of ‘Sanbu-sugi’ had only one genotype based on eight microsatellite loci. Infected Japanese cedars in Kyoto were comprised of different genotypes and showed no close relatedness to ‘Sanbu-sugi’. This is the first report of trunk rot caused by F. torreyae on cultivars of Japanese cedar with no relatedness to ‘Sanbu-sugi’ outside of Chiba and Ibaraki prefectures and showed the potential for expansion of this disease to Japanese cedar plantations in other areas. 相似文献
6.
Effect of bamboo vinegar on regulation of germination and radicle growth of seed plants 总被引:10,自引:0,他引:10
Two kinds of bamboo vinegar from madake bamboo (Phyllostachys bambusoides) and moso bamboo (Phyllostachys pubescens) were prepared to analyze their components by gas chromatography (GC). The original vinegar, distilled vinegar, ether-extracted
vinegar, and three fractions including acidic, neutral, and phenolic fractions separated from ether-extracted vinegar were
diluted with distilled water 102 to 107 times. These diluted vinegar solutions were used to investigate the effect of bamboo vinegar on the germination and radicle
growth of seed plants. High concentrations of all kinds of treated bamboo vinegars (e.g., 102 of original vinegar and 103 of ether-extracted vinegar) showed strong inhibition against germination of the seeds. However, an appropriate dilution of
bamboo vinegar showed an obvious promotional effect on germination and radicle growth for the four kinds of tested seeds (lettuce,
watercress, honewort, chrysanthemum).
Received: December, 12, 2001 / Accepted: July 29, 2002
RID="*"
ID="*" Part of this paper was presented at the 51st Annual Meeting of the Japan Wood Research Society, Tokyo, April 2001 相似文献
7.
Megumi Matsumoto Mahumoud Tanekey Amer Kyosuke Araki Atsushi Nishitani Kazuma Hayashi Yutaka Takeuchi Kazuhiro Shiozaki Atsushi Yamamoto 《Fisheries Science》2018,84(5):857-867
Interleukin-10 (IL-10) is an anti-inflammatory cytokine and negatively regulates cell-mediated immunity (CMI) induction by inhibiting cytokine production in type 1 T helper cells. IL-10 genes have been isolated from several fish, and inflammatory cytokine inhibition by IL-10 has been well examined. However, a CMI regulator of IL-10 in fish has not yet been identified. In this study, we cloned the IL-10 gene in amberjack Seriola dumerili and analyzed its function using its recombinant protein (rIL-10). In an in vitro culture experiment, gene expression of inflammatory cytokines was suppressed in leukocytes incubated with rIL-10 compared with cells that only received Nocardia seriolae stimulation. This result suggests amberjack IL-10 has conserved function as an inflammatory cytokine inhibitor. Bactericidal activity of amberjack cells against intracellular pathogen stimulation was decreased in a rIL-10 dose-dependent manner. Furthermore, a significant reduction in the T-bet/GATA-3 ratio was observed in N. seriolae living cell (LC)?+?rIL-10-injected fish. Taken together, these results suggest amberjack rIL-10 suppresses CMI induction both in vitro and in vivo. In addition, the number of IgM+ cells among spleen leukocytes in N. seriolae?+?rIL-10-injected fish was higher than in only N. seriolae LC, suggesting that Th2-dominant immunity was induced by adding rIL-10. 相似文献
8.
Haruo Yamaguchi Yuko Tanimoto Takamichi Yoshimatsu Shinya Sato Tomohiro Nishimura Keita Uehara Masao Adachi 《Fisheries Science》2012,78(5):993-1000
Blooms of toxic dinoflagellates of the genus Ostreopsis, which is known as a producer of palytoxin (PTX) analogs, may pose a threat to human health in tropical, subtropical, and temperate regions around the world. In the present study, we established a suitable culture method for Ostreopsis spp. isolated from Japanese coastal waters and characterized their growth potential using the method to discuss their bloom dynamics. Each clonal strain of Ostreopsis cf. ovata, Ostreopsis sp.?1, Ostreopsis sp.?5, and Ostreopsis sp.?6 was incubated in 25?×?150?mm test tubes with a flat bottom containing various kinds of medium. Since Ostreopsis spp. strains grew well in IMK and/or f/2 media, we selected these media for cultivation of all the Ostreopsis spp. isolates. Growth rates of O. cf. ovata (0.834?divisions/day), Ostreopsis sp.?1 (0.619?divisions/day), and Ostreopsis sp.?6 (1.04?divisions/day) that produce PTX analogs significantly differed (p?<?0.05) respectively and are clearly higher than those of other reported epiphytic dinoflagellate Gambierdiscus toxicus, Prorocentrum lima, and Coolia monotis cultures, which suggest that these species have ecological advantages to predominate through the algal succession in Japanese coastal waters, resulting in a potential risk to human health in this region. 相似文献
9.
Shinya Yano Megumi Mori Naozumi Teramoto Makoto Iisaka Natsumi Suzuki Masanari Noto Yasuko Kaimoto Masashi Kakimoto Michio Yamada Eri Shiratsuchi Toshiaki Shimasaki Mitsuhiro Shibata 《Marine drugs》2015,13(1):338-353
Photocrosslinked hydrogels reinforced by microfibrillated cellulose (MFC) were prepared from a methacrylate-functionalized fish elastin polypeptide and MFC dispersed in dimethylsulfoxide (DMSO). First, a water-soluble elastin peptide with a molecular weight of ca. 500 g/mol from the fish bulbus arteriosus was polymerized by N,N′-dicyclohexylcarbodiimide (DCC), a condensation reagent, and then modified with 2-isocyanatoethyl methacrylate (MOI) to yield a photocrosslinkable fish elastin polypeptide. The product was dissolved in DMSO and irradiated with UV light in the presence of a radical photoinitiator. We obtained hydrogels successfully by substitution of DMSO with water. The composite gel with MFC was prepared by UV irradiation of the photocrosslinkable elastin polypeptide mixed with dispersed MFC in DMSO, followed by substitution of DMSO with water. The tensile test of the composite gels revealed that the addition of MFC improved the tensile properties, and the shape of the stress–strain curve of the composite gel became more similar to the typical shape of an elastic material with an increase of MFC content. The rheology measurement showed that the elastic modulus of the composite gel increased with an increase of MFC content. The cell proliferation test on the composite gel showed no toxicity. 相似文献
10.
Megumi Yamagishi Ritsuko Hosoda-Yabe Hideki Tamai Miku Konishi Akihiro Imamura Hideharu Ishida Tomio Yabe Hiromune Ando Makoto Kiso 《Marine drugs》2015,13(12):7250-7274
LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling. 相似文献