全文获取类型
收费全文 | 125篇 |
免费 | 6篇 |
国内免费 | 1篇 |
专业分类
林业 | 1篇 |
农学 | 23篇 |
7篇 | |
综合类 | 15篇 |
农作物 | 5篇 |
水产渔业 | 2篇 |
畜牧兽医 | 71篇 |
园艺 | 1篇 |
植物保护 | 7篇 |
出版年
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 6篇 |
2013年 | 15篇 |
2012年 | 2篇 |
2011年 | 2篇 |
2010年 | 5篇 |
2009年 | 3篇 |
2008年 | 6篇 |
2007年 | 4篇 |
2006年 | 3篇 |
2005年 | 9篇 |
2004年 | 8篇 |
2003年 | 4篇 |
2002年 | 1篇 |
2001年 | 2篇 |
2000年 | 5篇 |
1999年 | 1篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1996年 | 3篇 |
1994年 | 4篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1991年 | 5篇 |
1990年 | 3篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1986年 | 2篇 |
1979年 | 1篇 |
1977年 | 2篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有132条查询结果,搜索用时 15 毫秒
1.
Adult wethers (n = 750) were lot-fed for 13 days, 8 days or 3 days before a simulated voyage lasting 18 days to examine whether the period of lot-feeding affected the proportion of sheep that ate pelleted feed and their body weight change during simulated shipping. There was no significant difference in the proportion of non-feeders between treatment groups on days 7 and 14 of the voyage. Body weights were not significantly different between the treatment groups on days 14 and 18 of the voyage. Overall body weight loss, from the farm to the end of simulated shipping, was 4.08 kg (+/- 0.28, s.e.m.), 4.58 kg (+/- 0.28) and 4.51 kg (+/- 0.28) in sheep lot-fed for 13 days, 8 days and 3 days, respectively, and was not significantly different between treatments. It was concluded that lot-feeding for 13 days conferred no advantage in body weight or numbers of non-feeders compared with shorter periods in this study. 相似文献
2.
SR Cho SA Ock JG Yoo B Mohana kumar SY Choe GJ Rho 《Reproduction in domestic animals》2005,40(2):171-176
The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5-10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 microM roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5-10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors. 相似文献
3.
Marais HJ Nel P Bertschinger HJ Schoeman JP Zimmerman D 《Journal of the South African Veterinary Association》2007,78(3):145-148
There are no reports in the literature describing any tumours, and specifically sarcoids, in zebras. The equine sarcoid, a locally aggressive, fibroblastic skin tumour, is the most common dermatological neoplasm reported in horses. The Cape mountain zebra (CMZ) has been described as one of the most vulnerable mammals in South Africa with current populations existing in isolated units. All South African CMZ are descendants from no more than 30 individual animals originating from 3 populations, namely the Mountain Zebra National Park, and Kammanassie and Gamka Mountain Nature Reserves near Cradock. The possibility therefore exists that the existing populations arose from a very small gene pool and that they are considerably inbred. A reduction in major histocompatibility complex diversity due to genetic bottlenecks and subsequent inbreeding probably contributed to uniform population sensitivity and the subsequent development of sarcoid in two CMZ populations, namely in the Bontebok National Park and Gariep Nature Reserve. The entire population of CMZ in the Bontebok National Park was observed and sampled during 2002 to document the prevalence and body distribution of sarcoids. During the same year, a comparative study was carried out on an outbred population of Burchell's zebra in the Kruger National Park. The prevalence in CMZ in the Bontebok National Park was 53 %, while the Burchell's zebra in Kruger National Park had a prevalence of 1.9 %. The most common sites for sarcoid in CMZ were the ventral abdomen and limbs. Prevalence of sarcoids in horses recorded in the literature varies between 0.5 % and 2 %. The Gariep Nature Reserve recently reported a prevalence of almost 25 % in CMZ in the reserve. 相似文献
4.
RT NORRIS CL McDONALD RB RICHARDS MW HYDER SP GITTINS GJ NORMAN 《Australian veterinary journal》1990,67(7):244-247
In the first of 2 experiments, a simulated voyage was conducted to examine the effects of various treatments on bodyweight change and feeding frequency of inappetant sheep at the end of lot-feeding (non-feeders). The treatments, applied during simulated shipping, were: normal quantities of feed and length of troughs; extra trough length; extra feed. Adult Merino wethers (n = 108) were used in each treatment. A voyage to the Middle East was then conducted to establish whether shipboard mortality could be reduced by separating non-feeders (n = 305) from feeders (n = 5,620) late in the feedlot hase and housing the groups separately aboard ship. A control group of non-feeders (n = 215) mixed with feeders (n = 5,732) was used for comparison. Bars (marker bars), containing a coloured dye, were attached to feed troughs to mark sheep that fed. Most non-feeders (82%) began eating when placed in shipping pens in both studies. However, there was no significant difference in percentage of sheep that fed between non-feeders given extra trough length or extra feed compared with non-feeders given standard management at any stage of simulated shipping. There was no significant difference in mean bodyweights between treatment groups on days 1, 8 and 15 of simulated shipping. Differences in bodyweight on d 22 were probably associated with different levels of gut fill. Death rates were not significantly different in separated and control groups (1.1%, 0.9%, P = 0.6) in the voyage of 14 d to the Middle East.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
5.
6.
Monensin controlled-release intraruminal capsule for control of bloat in pastured dairy cows 总被引:1,自引:0,他引:1
LB LOWE GJ BALL VR CARRUTHERS† RC DOBOS‡ GA LYNCH PJ MOATE PR POOLE† SC VALENTINE 《Australian veterinary journal》1991,68(1):17-20
Monensin, a polyether ionophore antibiotic, is potentially an important agent for bloat relief in dairy cows grazing temperate legume-based pasture. A series of studies was undertaken to determine the effect of monensin, when delivered continuously in the rumen of lactating dairy cows by means of controlled-release capsules (monensin CRC). Such devices release approximately 300 mg/head/day for 100 d. A short-term pilot study made at Ruakura, New Zealand, tested monensin CRC in cows selected for high susceptibility to bloat and grazing lucerne (Medicago sativa) or red clover (Trifolium pratense). Treatment significantly reduced the incidence of bloat, while milk yield and protein yield were increased. There was no effect on fat yield. Following the pilot study, 6 large-scale field experiments involving a total of 368 lactating dairy cows, were made in Australia and New Zealand to confirm the effectiveness of monensin CRC for bloat control and to measure the effect of such treatment on milk production and composition. A severe bloat problem occurred in 2 experiments, mild bloat occurred in 2 others, while no visual signs of bloat were observed in the remaining 2 experiments. Bloat was significantly (P less than 0.05) reduced by monensin CRC treatment when data was pooled over the 4 experiments in which bloat occurred. Daily milk yield was increased in all experiments from a mean of 17.7 in untreated groups to 18.8 kg/head/day (P less than 0.05) in monensin CRC-treated cows. Protein percentage was not affected by treatment, while there was a decrease from 4.29 to 4.10% fat, although total fat yield was not affected.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
7.
8.
Barbarita Companioni Mayda Arzola Yania Rodríguez Marais Mosqueda María Cristina Pérez Orlando Borrás José Carlos Lorenzo Ramón Santos 《Euphytica》2003,130(3):341-347
Banana and plantain are among the most important food crops in developing countries but production is threatened by increasing
virulent forms of Fusarium oxysporum f. sp. cubense. Chemical control is not economically effective and,therefore, breeding programs are necessary. Traditional field studies
of new genotype resistance to this disease are time-consuming and destructive. Therefore,we developed a rapid and non-destructive
procedure to differentiate field-grown banana resistant from susceptible clones. This procedure implicates application of
culture filtrates of Fusarium oxysporum f. sp. cubense race 1 onto banana leaves. The relationship between duration of the fungal in vitro incubation, and the fungal culture fresh mass, the culture filtrate absorbency, and the Gross Michel (susceptible cultivar)leaf
lesion area (after application of the culture filtrate) were similar and at 24day-incubation the highest values of the recorded
indicators were observed. A comparison between Gross Michel and FHIA-01(resistant) was also performed. The most relevant differences
between cultivars were observed at 48 hours after application of the culture filtrate, and in the middle-aged leaves. The
position of the culture filtrate application in the leaf limb (distal, middle, proximal) was not determinant. A wider comparison
among banana cultivars confirmed previous results informed by other researchers using different systems to study this plant-fungus
interaction. Such a confirmation validates the effectiveness of the procedure described here to select rapid and non-destructively
banana resistance to this disease at field level.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
9.
G. F. Marais 《Plant Breeding》1988,100(2):157-159
Pollination of ‘Chinese Spring,’ monosome 1D plants with rye results in failure of hybrid seed development in a proportion of the F1 seeds corresponding to the transmission rate of the nullisomic 1D egg cells. Development and viability of these hybrid seeds closely resemble that normally observed in T. aurum× rye crosses. Using ‘Chinese Spring’ chromosome ID telosomic plants in crosses with rye, it was possible to illustrate that the observed effect was associated with the long arm of this chromosome. 相似文献
10.
Grant JA Arvidson R Bell JF Cabrol NA Carr MH Christensen P Crumpler L Des Marais DJ Ehlmann BL Farmer J Golombek M Grant FD Greeley R Herkenhoff K Li R McSween HY Ming DW Moersch J Rice JW Ruff S Richter L Squyres S Sullivan R Weitz C 《Science (New York, N.Y.)》2004,305(5685):807-810
The Mars Exploration Rover Spirit has traversed a fairly flat, rock-strewn terrain whose surface is shaped primarily by impact events, although some of the landscape has been altered by eolian processes. Impacts ejected basaltic rocks that probably were part of locally formed lava flows from at least 10 meters depth. Some rocks have been textured and/or partially buried by windblown sediments less than 2 millimeters in diameter that concentrate within shallow, partially filled, circular impact depressions referred to as hollows. The terrain traversed during the 90-sol (martian solar day) nominal mission shows no evidence for an ancient lake in Gusev crater. 相似文献